U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Zfat zinc finger and AT hook domain containing [ Mus musculus (house mouse) ]

Gene ID: 380993, updated on 9-May-2024

Summary

Go to the top of the page Help
Official Symbol
Zfatprovided by MGI
Official Full Name
zinc finger and AT hook domain containingprovided by MGI
Primary source
MGI:MGI:2681865
See related
Ensembl:ENSMUSG00000022335 AllianceGenome:MGI:2681865
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Gm922; Zfat1; Zfp406
Summary
Enables DNA-binding transcription activator activity, RNA polymerase II-specific. Acts upstream of or within hematopoietic progenitor cell differentiation and spongiotrophoblast layer development. Predicted to be located in nucleus. Is expressed in several structures, including extraembryonic component; nervous system; and placenta. Human ortholog(s) of this gene implicated in autoimmune thyroiditis. Orthologous to human ZFAT (zinc finger and AT-hook domain containing). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in ovary adult (RPKM 1.9), placenta adult (RPKM 1.6) and 28 other tissues See more
Orthologs
human all
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Location:
15 D2; 15 29.44 cM
Exon count:
19
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 15 NC_000081.7 (67955563..68130771, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 15 NC_000081.6 (68083714..68258922, complement)

Chromosome 15 - NC_000081.7Genomic Context describing neighboring genes Neighboring gene RIKEN cDNA 1700012I11 gene Neighboring gene STARR-seq mESC enhancer starr_38899 Neighboring gene STARR-seq mESC enhancer starr_38900 Neighboring gene STARR-seq mESC enhancer starr_38902 Neighboring gene STARR-seq mESC enhancer starr_38903 Neighboring gene STARR-seq mESC enhancer starr_38904 Neighboring gene predicted gene, 53906 Neighboring gene STARR-seq mESC enhancer starr_38905 Neighboring gene STARR-positive B cell enhancer ABC_E7377 Neighboring gene ribosomal protein L21 pseudogene Neighboring gene predicted gene 20732 Neighboring gene microRNA 30b

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Zfat expression in ZsGreen reporter gene knock‑in mice: Implications for a novel function of Zfat in definitive erythropoiesis. Tsunoda T, et al. Int J Mol Med, 2018 Nov. PMID 30106088, Free PMC Article
  2. Molecular mechanisms of transcriptional regulation by the nuclear zinc-finger protein Zfat in T cells. Ishikura S, et al. Biochim Biophys Acta, 2016 Nov. PMID 27591365
  3. Zfat-deficient CD4⁺ CD8⁺ double-positive thymocytes are susceptible to apoptosis with deregulated activation of p38 and JNK. Ishikura S, et al. J Cell Biochem, 2015 Jan. PMID 25169027
  4. ZFAT plays critical roles in peripheral T cell homeostasis and its T cell receptor-mediated response. Doi K, et al. Biochem Biophys Res Commun, 2012 Aug 17. PMID 22828507
  5. ZFAT is a critical molecule for cell survival in mouse embryonic fibroblasts. Doi K, et al. Cell Mol Biol Lett, 2011 Mar. PMID 21225468, Free PMC Article

See all (29) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. CENP-B promotes the centromeric localization of ZFAT to control transcription of noncoding RNA.
    Title: CENP-B promotes the centromeric localization of ZFAT to control transcription of noncoding RNA.
  2. ZFAT binds to centromeres to control noncoding RNA transcription through the KAT2B-H4K8ac-BRD4 axis.
    Title: ZFAT binds to centromeres to control noncoding RNA transcription through the KAT2B-H4K8ac-BRD4 axis.
  3. Zfat knockdown partially inhibited CD71(-/low)Ter119(-) to CD71(high)Ter119(-) transition of fetal liver erythroid progenitors with impairment in the elevation of CD71 expression. Zfat plays a critical role for erythropoiesis in the fetal liver
    Title: Zfat Is Indispensable for the Development of Erythroid Cells in the Fetal Liver.
  4. findings demonstrated that Zfat regulates the transcription of target genes by binding directly to the TSS proximal region, and that Zfat-target genes play important roles in T-cell homeostasis
    Title: Molecular mechanisms of transcriptional regulation by the nuclear zinc-finger protein Zfat in T cells.
  5. Zfat-deficient T cells show increased numbers of autophagosomes and decreased levels of p62 protein, together indicating that Zfat deficiency promotes lysosomal FoxO1 degradation through autophagy.
    Title: The Nuclear Zinc Finger Protein Zfat Maintains FoxO1 Protein Levels in Peripheral T Cells by Regulating the Activities of Autophagy and the Akt Signaling Pathway.
  6. results demonstrate that Zfat critically participates in the development of DP thymocytes through regulating the activities of p38 and JNK.
    Title: Zfat-deficient CD4⁺ CD8⁺ double-positive thymocytes are susceptible to apoptosis with deregulated activation of p38 and JNK.
  7. Zfat is required for peripheral T-cell homeostasis through IL-7Ralpha expression by controlling the FoxO1 protein.
    Title: Marked Reduction in FoxO1 Protein by its Enhanced Proteasomal Degradation in Zfat-deficient Peripheral T-Cells.
  8. Zfat-deficiency results in a loss of CD3zeta phosphorylation with dysregulation of ERK and Egr activities leading to impaired positive selection.
    Title: Zfat-deficiency results in a loss of CD3ζ phosphorylation with dysregulation of ERK and Egr activities leading to impaired positive selection.
  9. ZFAT is a critical molecule for cell survival in MEFs and MOLT-4 cells at least in part through the regulation of the apoptosis involved in the BCL-2- and IL6st-mediated pathways.
    Title: ZFAT is a critical molecule for cell survival in mouse embryonic fibroblasts.
  10. ZFAT is indispensable for mouse embryonic development and functions as a critical transcription factor for primitive hematopoiesis through direct-regulation of Tal1, Lmo2, and Gata1
    Title: Immune-related zinc finger gene ZFAT is an essential transcriptional regulator for hematopoietic differentiation in blood islands.
Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

Go to the top of the page Help

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (3)  1 citation
  • Endonuclease-mediated (2) 

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Markers

Homology

Clone Names

  • MGC56951

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription activator activity, RNA polymerase II-specific IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA-binding transcription factor activity, RNA polymerase II-specific IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within hematopoietic progenitor cell differentiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within hemopoiesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of DNA-templated transcription IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within spongiotrophoblast layer development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus IC
Inferred by Curator
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
zinc finger protein ZFAT
Names
ZFAT zinc finger 1
zinc finger protein 403
zinc finger protein 406

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001145888.2 → NP_001139360.1  zinc finger protein ZFAT isoform 1

    See identical proteins and their annotated locations for NP_001139360.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AC108821, AC126249, AC140212
    Consensus CDS
    CCDS49623.1
    UniProtKB/Swiss-Prot
    B2C322, Q7TS63
    UniProtKB/TrEMBL
    E0CX30
    Related
    ENSMUSP00000125257.2, ENSMUST00000160248.8
    Conserved Domains (4) summary
    sd00017
    Location:273 → 293
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:271 → 293
    zf-C2H2; Zinc finger, C2H2 type
    pfam13909
    Location:299 → 323
    zf-H2C2_5; C2H2-type zinc-finger domain
    pfam13465
    Location:285 → 309
    zf-H2C2_2; Zinc-finger double domain

  2. NM_001403039.1 → NP_001389968.1  zinc finger protein ZFAT isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC108821, AC126249, AC140212

  3. NM_198644.3 → NP_941046.1  zinc finger protein ZFAT isoform 2

    See identical proteins and their annotated locations for NP_941046.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) uses an alternate in-frame splice site in both the 5' and 3' coding regions, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.
    Source sequence(s)
    AC108821, AC126249, AC140212
    Consensus CDS
    CCDS49624.1
    UniProtKB/TrEMBL
    E0CX30
    Related
    ENSMUSP00000125732.2, ENSMUST00000162054.9
    Conserved Domains (3) summary
    sd00017
    Location:266 → 286
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:264 → 286
    zf-C2H2; Zinc finger, C2H2 type
    pfam13465
    Location:278 → 300
    zf-H2C2_2; Zinc-finger double domain

RNA

  1. NR_152182.1 RNA Sequence

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) lacks two alternate internal exons, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    AC108821, AC126249, AC140212

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000081.7 Reference GRCm39 C57BL/6J

    Range
    67955563..68130771 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006521124.4 → XP_006521187.2  zinc finger protein ZFAT isoform X1

    UniProtKB/TrEMBL
    E0CX30
    Conserved Domains (3) summary
    sd00017
    Location:318 → 338
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:316 → 338
    zf-C2H2; Zinc finger, C2H2 type
    pfam13909
    Location:344 → 368
    zf-H2C2_5; C2H2-type zinc-finger domain

  2. XM_030248622.2 → XP_030104482.1  zinc finger protein ZFAT isoform X3

    UniProtKB/TrEMBL
    E0CX30
    Conserved Domains (3) summary
    sd00017
    Location:261 → 281
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:259 → 281
    zf-C2H2; Zinc finger, C2H2 type
    pfam13909
    Location:287 → 311
    zf-H2C2_5; C2H2-type zinc-finger domain

  3. XM_011245683.4 → XP_011243985.1  zinc finger protein ZFAT isoform X3

    UniProtKB/TrEMBL
    E0CX30
    Conserved Domains (3) summary
    sd00017
    Location:261 → 281
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:259 → 281
    zf-C2H2; Zinc finger, C2H2 type
    pfam13909
    Location:287 → 311
    zf-H2C2_5; C2H2-type zinc-finger domain

RNA

  1. XR_003951402.2 RNA Sequence

  2. XR_004938848.1 RNA Sequence

  3. XR_004938847.1 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q7TS63.2 GenPept UniProtKB/Swiss-Prot:Q7TS63

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Chemical Information
Molecular Biology Databases
Research Materials
Tools
Miscellaneous