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Hecw2 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 [ Mus musculus (house mouse) ]

Gene ID: 329152, updated on 21-Apr-2024

Summary

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Official Symbol
Hecw2provided by MGI
Official Full Name
HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2provided by MGI
Primary source
MGI:MGI:2685817
See related
Ensembl:ENSMUSG00000042807 AllianceGenome:MGI:2685817
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Gm971; Nedl2; mKIAA1301; A730039N16Rik; D030049F17Rik
Summary
Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in several processes, including cellular protein metabolic process; negative regulation of sodium ion transmembrane transporter activity; and regulation of dendrite morphogenesis. Predicted to be located in mitotic spindle. Predicted to be active in cytoplasm. Orthologous to human HECW2 (HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in cortex adult (RPKM 2.4), lung adult (RPKM 2.2) and 19 other tissues See more
Orthologs
human all
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Genomic context

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Location:
1 C1.1; 1 26.99 cM
Exon count:
32
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (53846031..54234193, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (53806872..54195034, complement)

Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 53553 Neighboring gene dynein, axonemal, heavy chain 7A Neighboring gene STARR-seq mESC enhancer starr_00916 Neighboring gene STARR-seq mESC enhancer starr_00917 Neighboring gene STARR-seq mESC enhancer starr_00918 Neighboring gene STARR-seq mESC enhancer starr_00919 Neighboring gene STARR-seq mESC enhancer starr_00920 Neighboring gene STARR-positive B cell enhancer ABC_E7724 Neighboring gene STARR-seq mESC enhancer starr_00921 Neighboring gene STARR-seq mESC enhancer starr_00924 Neighboring gene predicted gene, 32311 Neighboring gene STARR-positive B cell enhancer ABC_E627 Neighboring gene STARR-positive B cell enhancer ABC_E1544 Neighboring gene STARR-positive B cell enhancer ABC_E3276 Neighboring gene STARR-seq mESC enhancer starr_00925 Neighboring gene serine/threonine kinase 17b (apoptosis-inducing) Neighboring gene STARR-positive B cell enhancer ABC_E7726 Neighboring gene STARR-positive B cell enhancer ABC_E5754 Neighboring gene STARR-seq mESC enhancer starr_00926 Neighboring gene CapStarr-seq enhancer MGSCv37_chr1:53951535-53951718 Neighboring gene STARR-positive B cell enhancer ABC_E9464 Neighboring gene microRNA 7681 Neighboring gene predicted gene, 24251 Neighboring gene STARR-seq mESC enhancer starr_00927 Neighboring gene STARR-seq mESC enhancer starr_00928 Neighboring gene STARR-seq mESC enhancer starr_00929 Neighboring gene STARR-seq mESC enhancer starr_00930 Neighboring gene STARR-seq mESC enhancer starr_00931 Neighboring gene STARR-seq mESC enhancer starr_00932 Neighboring gene predicted gene, 51622 Neighboring gene predicted gene, 25829 Neighboring gene coiled-coil domain containing 150 Neighboring gene STARR-seq mESC enhancer starr_00933

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Engagement of circular RNA HECW2 in the nonautophagic role of ATG5 implicated in the endothelial-mesenchymal transition. Yang L, et al. Autophagy, 2018. PMID 29260931, Free PMC Article
  2. The kinase Grk2 regulates Nedd4/Nedd4-2-dependent control of epithelial Na+ channels. Dinudom A, et al. Proc Natl Acad Sci U S A, 2004 Aug 10. PMID 15284439, Free PMC Article
  3. NEDL1, a novel ubiquitin-protein isopeptide ligase for dishevelled-1, targets mutant superoxide dismutase-1. Miyazaki K, et al. J Biol Chem, 2004 Mar 19. PMID 14684739
  4. A novel HECT-type E3 ubiquitin ligase, NEDL2, stabilizes p73 and enhances its transcriptional activity. Miyazaki K, et al. Biochem Biophys Res Commun, 2003 Aug 15. PMID 12890487
  5. NEDL2 is an essential regulator of enteric neural development and GDNF/Ret signaling. Wei R, et al. Cell Signal, 2015 Mar. PMID 25555806

See all (23) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. that circHECW2 regulated the EndoMT by directly binding to MIR30D, a significantly downregulated miRNA from miRNA profiling, which subsequently caused an increased expression of ATG5
    Title: Engagement of circular RNA HECW2 in the nonautophagic role of ATG5 implicated in the endothelial-mesenchymal transition.
  2. Data show that NEDL2 regulates GDNF/Ret/Akt pathway depends on its Nedd8 ligase activity rather than ubiquitin ligase activity.
    Title: NEDL2 regulates enteric nervous system and kidney development in its Nedd8 ligase activity-dependent manner.
  3. NEDL2 positively regulates enteric neural precursor proliferation through the GDNF/Akt signaling pathway.
    Title: NEDL2 is an essential regulator of enteric neural development and GDNF/Ret signaling.
  4. Grk2 phosphorylates the C terminus of the Na-channel beta subunit and renders the channels insensitive to inhibition by Nedd4-2
    Title: The kinase Grk2 regulates Nedd4/Nedd4-2-dependent control of epithelial Na+ channels.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Gene trapped (1) 
  • Targeted (3)  1 citation
  • Endonuclease-mediated (2) 

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ubiquitin-protein transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in negative regulation of sodium ion transmembrane transporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein ubiquitination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of dendrite morphogenesis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of mitotic metaphase/anaphase transition ISO
Inferred from Sequence Orthology
more info
  
involved_in ubiquitin-dependent protein catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
  
located_in mitotic spindle ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
E3 ubiquitin-protein ligase HECW2
Names
HECT, C2 and WW domain-containing protein 2
HECT-type E3 ubiquitin transferase HECW2
NEDD4-like E3 ubiquitin-protein ligase 2
NEDD4-related E3 ubiquitin ligase NEDL2
NP_001001883.1
NP_766243.2
XP_006496129.1
XP_006496130.1
XP_006496131.1
XP_006496132.1
XP_011236839.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001001883.3 → NP_001001883.1  E3 ubiquitin-protein ligase HECW2 isoform 1

    See identical proteins and their annotated locations for NP_001001883.1

    Status: VALIDATED

    Source sequence(s)
    AC124175, AC136517, AC150896
    Consensus CDS
    CCDS14956.1
    UniProtKB/Swiss-Prot
    Q3KNL8, Q6I6G8, Q6ZPU4, Q8C956
    Related
    ENSMUSP00000084942.5, ENSMUST00000087659.11
    Conserved Domains (7) summary
    pfam06390
    Location:463 → 639
    NESP55; Neuroendocrine-specific golgi protein P55 (NESP55)
    cd08691
    Location:185 → 320
    C2_NEDL1-like; C2 domain present in NEDL1 (NEDD4-like ubiquitin protein ligase-1)
    cd00078
    Location:1222 → 1576
    HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
    pfam00397
    Location:815 → 844
    WW; WW domain
    pfam07263
    Location:344 → 700
    DMP1; Dentin matrix protein 1 (DMP1)
    pfam16562
    Location:45 → 164
    HECW_N; N-terminal domain of E3 ubiquitin-protein ligase HECW1 and 2
    pfam18436
    Location:921 → 987
    HECW1_helix; Helical box domain of E3 ubiquitin-protein ligase HECW1

  2. NM_172655.3 → NP_766243.2  E3 ubiquitin-protein ligase HECW2 isoform 2

    See identical proteins and their annotated locations for NP_766243.2

    Status: VALIDATED

    Source sequence(s)
    AC150896, AK042922
    Consensus CDS
    CCDS14957.1
    UniProtKB/TrEMBL
    A3KPB7
    Related
    ENSMUSP00000095348.3, ENSMUST00000097741.3
    Conserved Domains (2) summary
    pfam16562
    Location:45 → 164
    HECW_N; N-terminal domain of E3 ubiquitin-protein ligase HECW1 and 2
    cl14603
    Location:185 → 294
    C2; C2 domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000067.7 Reference GRCm39 C57BL/6J

    Range
    53846031..54234193 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006496067.3 → XP_006496130.1  E3 ubiquitin-protein ligase HECW2 isoform X1

    See identical proteins and their annotated locations for XP_006496130.1

    UniProtKB/Swiss-Prot
    Q3KNL8, Q6I6G8, Q6ZPU4, Q8C956
    Conserved Domains (7) summary
    pfam06390
    Location:463 → 639
    NESP55; Neuroendocrine-specific golgi protein P55 (NESP55)
    cd08691
    Location:185 → 320
    C2_NEDL1-like; C2 domain present in NEDL1 (NEDD4-like ubiquitin protein ligase-1)
    cd00078
    Location:1222 → 1576
    HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
    pfam00397
    Location:815 → 844
    WW; WW domain
    pfam07263
    Location:344 → 700
    DMP1; Dentin matrix protein 1 (DMP1)
    pfam16562
    Location:45 → 164
    HECW_N; N-terminal domain of E3 ubiquitin-protein ligase HECW1 and 2
    pfam18436
    Location:921 → 987
    HECW1_helix; Helical box domain of E3 ubiquitin-protein ligase HECW1

  2. XM_006496068.5 → XP_006496131.1  E3 ubiquitin-protein ligase HECW2 isoform X1

    See identical proteins and their annotated locations for XP_006496131.1

    UniProtKB/Swiss-Prot
    Q3KNL8, Q6I6G8, Q6ZPU4, Q8C956
    Conserved Domains (7) summary
    pfam06390
    Location:463 → 639
    NESP55; Neuroendocrine-specific golgi protein P55 (NESP55)
    cd08691
    Location:185 → 320
    C2_NEDL1-like; C2 domain present in NEDL1 (NEDD4-like ubiquitin protein ligase-1)
    cd00078
    Location:1222 → 1576
    HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
    pfam00397
    Location:815 → 844
    WW; WW domain
    pfam07263
    Location:344 → 700
    DMP1; Dentin matrix protein 1 (DMP1)
    pfam16562
    Location:45 → 164
    HECW_N; N-terminal domain of E3 ubiquitin-protein ligase HECW1 and 2
    pfam18436
    Location:921 → 987
    HECW1_helix; Helical box domain of E3 ubiquitin-protein ligase HECW1

  3. XM_006496066.5 → XP_006496129.1  E3 ubiquitin-protein ligase HECW2 isoform X1

    See identical proteins and their annotated locations for XP_006496129.1

    UniProtKB/Swiss-Prot
    Q3KNL8, Q6I6G8, Q6ZPU4, Q8C956
    Related
    ENSMUSP00000113283.2, ENSMUST00000120904.8
    Conserved Domains (7) summary
    pfam06390
    Location:463 → 639
    NESP55; Neuroendocrine-specific golgi protein P55 (NESP55)
    cd08691
    Location:185 → 320
    C2_NEDL1-like; C2 domain present in NEDL1 (NEDD4-like ubiquitin protein ligase-1)
    cd00078
    Location:1222 → 1576
    HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
    pfam00397
    Location:815 → 844
    WW; WW domain
    pfam07263
    Location:344 → 700
    DMP1; Dentin matrix protein 1 (DMP1)
    pfam16562
    Location:45 → 164
    HECW_N; N-terminal domain of E3 ubiquitin-protein ligase HECW1 and 2
    pfam18436
    Location:921 → 987
    HECW1_helix; Helical box domain of E3 ubiquitin-protein ligase HECW1

  4. XM_006496069.5 → XP_006496132.1  E3 ubiquitin-protein ligase HECW2 isoform X2

    See identical proteins and their annotated locations for XP_006496132.1

    Conserved Domains (7) summary
    pfam06390
    Location:332 → 508
    NESP55; Neuroendocrine-specific golgi protein P55 (NESP55)
    cd08691
    Location:54 → 189
    C2_NEDL1-like; C2 domain present in NEDL1 (NEDD4-like ubiquitin protein ligase-1)
    cd00078
    Location:1091 → 1445
    HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
    pfam00397
    Location:684 → 713
    WW; WW domain
    pfam07263
    Location:213 → 569
    DMP1; Dentin matrix protein 1 (DMP1)
    pfam16562
    Location:1 → 33
    HECW_N; N-terminal domain of E3 ubiquitin-protein ligase HECW1 and 2
    pfam18436
    Location:790 → 856
    HECW1_helix; Helical box domain of E3 ubiquitin-protein ligase HECW1

  5. XM_011238537.4 → XP_011236839.1  E3 ubiquitin-protein ligase HECW2 isoform X2

    See identical proteins and their annotated locations for XP_011236839.1

    Conserved Domains (7) summary
    pfam06390
    Location:332 → 508
    NESP55; Neuroendocrine-specific golgi protein P55 (NESP55)
    cd08691
    Location:54 → 189
    C2_NEDL1-like; C2 domain present in NEDL1 (NEDD4-like ubiquitin protein ligase-1)
    cd00078
    Location:1091 → 1445
    HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
    pfam00397
    Location:684 → 713
    WW; WW domain
    pfam07263
    Location:213 → 569
    DMP1; Dentin matrix protein 1 (DMP1)
    pfam16562
    Location:1 → 33
    HECW_N; N-terminal domain of E3 ubiquitin-protein ligase HECW1 and 2
    pfam18436
    Location:790 → 856
    HECW1_helix; Helical box domain of E3 ubiquitin-protein ligase HECW1
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q6I6G8.1 GenPept UniProtKB/Swiss-Prot:Q6I6G8

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