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Mical2 microtubule associated monooxygenase, calponin and LIM domain containing 2 [ Mus musculus (house mouse) ]

Gene ID: 320878, updated on 5-Mar-2024

Summary

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Official Symbol
Mical2provided by MGI
Official Full Name
microtubule associated monooxygenase, calponin and LIM domain containing 2provided by MGI
Primary source
MGI:MGI:2444947
See related
Ensembl:ENSMUSG00000038244 AllianceGenome:MGI:2444947
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
MICAL-2; Micalcl; mKIAA0750; 9530064J02; 4921517J23Rik; 5330438E18Rik
Summary
Enables several functions, including NAD(P)H oxidase H2O2-forming activity; actin binding activity; and mitogen-activated protein kinase binding activity. Involved in actin filament depolymerization and sulfur oxidation. Located in cytoplasm. Is expressed in lower jaw molar and upper jaw molar. Orthologous to human MICAL2 (microtubule associated monooxygenase, calponin and LIM domain containing 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in cortex adult (RPKM 29.1), lung adult (RPKM 19.7) and 20 other tissues See more
Orthologs
human all
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Genomic context

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Location:
7 F1; 7 58.75 cM
Exon count:
39
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 7 NC_000073.7 (111825032..112012311)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 7 NC_000073.6 (112225825..112355194)

Chromosome 7 - NC_000073.7Genomic Context describing neighboring genes Neighboring gene CapStarr-seq enhancer MGSCv37_chr7:119351859-119351968 Neighboring gene STARR-seq mESC enhancer starr_19854 Neighboring gene predicted gene, 39070 Neighboring gene predicted gene, 33247 Neighboring gene VISTA enhancer mm79 Neighboring gene CapStarr-seq enhancer MGSCv37_chr7:119459929-119460130 Neighboring gene STARR-seq mESC enhancer starr_19855 Neighboring gene predicted gene, 51494 Neighboring gene STARR-seq mESC enhancer starr_19858 Neighboring gene parvin, alpha Neighboring gene predicted gene, 45998 Neighboring gene STARR-seq mESC enhancer starr_19860 Neighboring gene parvin, alpha, opposite strand

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. MICAL2 regulates myofibroblasts differentiation in epidural fibrosis via SRF/MRTF-A signaling pathway. Jiang F, et al. Life Sci, 2021 Mar 15. PMID 33453238
  2. MICAL2 is essential for myogenic lineage commitment. Giarratana N, et al. Cell Death Dis, 2020 Aug 18. PMID 32811811, Free PMC Article
  3. Identification of ERK2-binding domain of EBITEIN1, a novel ERK2-binding protein. Miura K, et al. Biochim Biophys Acta, 2008 Sep. PMID 18590835
  4. ERK2-binding domain is required for phosphorylation of EBITEIN1, a potential downstream interactor of ERK2. Miura K. Biochem Biophys Res Commun, 2008 Oct 24. PMID 18700133
  5. Actin stimulates reduction of the MICAL-2 monooxygenase domain. McDonald CA, et al. Biochemistry, 2013 Sep 3. PMID 23927065

See all (28) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. EBITEIN1 is a phosphoprotein and a downstream interactor of ERK2 that participates in the intracellular signal transduction pathway mediating the morphogenetic development of round spermatids into spermatozoa.
    Title: ERK2-binding domain is required for phosphorylation of EBITEIN1, a potential downstream interactor of ERK2.
  2. Based on empirical and bioinformatic analyses, we propose two domains in EBITEIN1. One is the minimal sequence for binding ERK2 (EB domain) and the other is the EBITEIN1 C-terminal domain (ECT domain).
    Title: Identification of ERK2-binding domain of EBITEIN1, a novel ERK2-binding protein.
  3. MICAL2 is essential for myogenic lineage commitment.
    Title: MICAL2 is essential for myogenic lineage commitment.
  4. MICAL2 regulates myofibroblasts differentiation in epidural fibrosis via SRF/MRTF-A signaling pathway.
    Title: MICAL2 regulates myofibroblasts differentiation in epidural fibrosis via SRF/MRTF-A signaling pathway.
  5. In pulmonary artery hypertension miR-205-5p suppressed pulmonary artery smooth muscle cell proliferation by targeting MICAL2, which activated ERK1/2 signaling.
    Title: miR-205-5p suppresses pulmonary vascular smooth muscle cell proliferation by targeting MICAL2-mediated Erk1/2 signaling.
  6. show that this strategy is used by MICAL-2. Thus, our data suggest that MICAL-2 could regulate catalysis through the monooxygenase domain alone
    Title: Actin stimulates reduction of the MICAL-2 monooxygenase domain.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (1) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • KIAA0750, MGC32457

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables FAD binding IEA
Inferred from Electronic Annotation
more info
  
enables NAD(P)H oxidase H2O2-forming activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables actin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables actin binding ISO
Inferred from Sequence Orthology
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables mitogen-activated protein kinase binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables monooxygenase activity ISO
Inferred from Sequence Orthology
more info
  
enables oxidoreductase activity ISO
Inferred from Sequence Orthology
more info
  
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in actin filament depolymerization IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in actin filament depolymerization ISO
Inferred from Sequence Orthology
more info
  
involved_in cytoskeleton organization ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of transcription by RNA polymerase II ISO
Inferred from Sequence Orthology
more info
  
involved_in sulfur oxidation IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
is_active_in actin filament ISO
Inferred from Sequence Orthology
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
F-actin-monooxygenase MICAL2; [F-actin]-monooxygenase MICAL2
Names
ERK2-binding testicular protein 1
MICAL C-terminal like
MICAL C-terminal-like protein
MICAL C-terminal-like protein-like
[F-actin]-methionine sulfoxide oxidase MICAL2
ebitein-1
ebitein1
mMical2
mical-cL
microtubule associated monoxygenase, calponin and LIM domain containing 2
molecule interacting with CasL protein 2
protein MICAL-2
protein-methionine sulfoxide oxidase MICAL2
NP_001180234.1
NP_001368791.1
NP_001382793.1
NP_796256.1
XP_006508017.1
XP_030098545.1
XP_036009064.1
XP_036009065.1
XP_036009066.1
XP_036009067.1
XP_036009068.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001193305.1 → NP_001180234.1  [F-actin]-monooxygenase MICAL2 isoform A

    See identical proteins and their annotated locations for NP_001180234.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (A) encodes the longer isoform (A).
    Source sequence(s)
    AC100153, AC132392, AC166834
    Consensus CDS
    CCDS57581.1
    UniProtKB/Swiss-Prot
    Q8BML1
    Related
    ENSMUSP00000047639.6, ENSMUST00000037991.12
    Conserved Domains (3) summary
    cd09439
    Location:980 → 1034
    LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)
    cl30692
    Location:87 → 224
    UbiH; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases [Coenzyme transport and metabolism, Energy production and conversion]
    pfam00307
    Location:522 → 623
    CH; Calponin homology (CH) domain

  2. NM_001381862.1 → NP_001368791.1  [F-actin]-monooxygenase MICAL2 isoform D

    Status: VALIDATED

    Source sequence(s)
    AC132392
    Consensus CDS
    CCDS52364.1
    UniProtKB/TrEMBL
    A0A1L1ST75
    Related
    ENSMUSP00000102256.2, ENSMUST00000106645.8
    Conserved Domains (1) summary
    pfam12130
    Location:532 → 660
    DUF3585; Protein of unknown function (DUF3585)

  3. NM_001395864.1 → NP_001382793.1  [F-actin]-monooxygenase MICAL2 isoform C

    Status: VALIDATED

    Source sequence(s)
    AC100153, AC132392, AC166834
    UniProtKB/Swiss-Prot
    B1B545, F6ZD54, F8WJF1, Q14DP1, Q3TR48, Q3UPU6, Q5DU17, Q8BML1, Q8CID1, Q9D5U9
    Conserved Domains (5) summary
    cd09439
    Location:753 → 807
    LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)
    smart00033
    Location:520 → 617
    CH; Calponin homology domain
    cl30692
    Location:87 → 224
    UbiH; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases [Coenzyme transport and metabolism, Energy production and conversion]
    pfam09731
    Location:1295 → 1670
    Mitofilin; Mitochondrial inner membrane protein
    pfam17380
    Location:1514 → 1667
    DUF5401; Family of unknown function (DUF5401)

  4. NM_177282.5 → NP_796256.1  [F-actin]-monooxygenase MICAL2 isoform B

    See identical proteins and their annotated locations for NP_796256.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (B) lacks 5 alternate exons, resulting in the loss of an in-frame segment in the 3' coding region, and uses an alternate splice site in the 3' coding region, compared to variant 1. The resulting protein (isoform B) is shorter and has a distinct C-terminus, compared to isoform A.
    Source sequence(s)
    AC132392, AK030608, BB662540
    Consensus CDS
    CCDS21753.1
    UniProtKB/Swiss-Prot
    Q8BML1
    Related
    ENSMUSP00000051163.6, ENSMUST00000050149.12
    Conserved Domains (4) summary
    cd09439
    Location:753 → 807
    LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)
    pfam00307
    Location:522 → 617
    CH; Calponin homology (CH) domain
    pfam01494
    Location:87 → 274
    FAD_binding_3; FAD binding domain
    cl21454
    Location:91 → 121
    NADB_Rossmann; Rossmann-fold NAD(P)(+)-binding proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000073.7 Reference GRCm39 C57BL/6J

    Range
    111825032..112012311
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006507954.2 → XP_006508017.1  F-actin-monooxygenase MICAL2 isoform X1

    See identical proteins and their annotated locations for XP_006508017.1

    UniProtKB/Swiss-Prot
    Q8BML1
    Conserved Domains (3) summary
    cd09439
    Location:980 → 1034
    LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)
    cl30692
    Location:87 → 224
    UbiH; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases [Coenzyme transport and metabolism, Energy production and conversion]
    pfam00307
    Location:522 → 623
    CH; Calponin homology (CH) domain

  2. XM_036153171.1 → XP_036009064.1  F-actin-monooxygenase MICAL2 isoform X2

    Conserved Domains (3) summary
    cd09439
    Location:938 → 992
    LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)
    pfam01494
    Location:87 → 274
    FAD_binding_3; FAD binding domain
    cd21250
    Location:514 → 623
    CH_MICAL2; calponin homology (CH) domain found in molecule interacting with CasL protein 2

  3. XM_036153172.1 → XP_036009065.1  F-actin-monooxygenase MICAL2 isoform X3

    Conserved Domains (3) summary
    cd09439
    Location:873 → 927
    LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)
    pfam01494
    Location:87 → 274
    FAD_binding_3; FAD binding domain
    cd21250
    Location:514 → 623
    CH_MICAL2; calponin homology (CH) domain found in molecule interacting with CasL protein 2

  4. XM_036153173.1 → XP_036009066.1  F-actin-monooxygenase MICAL2 isoform X4

    Conserved Domains (3) summary
    cd09439
    Location:831 → 885
    LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)
    pfam01494
    Location:87 → 274
    FAD_binding_3; FAD binding domain
    cd21250
    Location:514 → 623
    CH_MICAL2; calponin homology (CH) domain found in molecule interacting with CasL protein 2

  5. XM_036153174.1 → XP_036009067.1  F-actin-monooxygenase MICAL2 isoform X5

    Conserved Domains (3) summary
    cd09439
    Location:789 → 843
    LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)
    pfam01494
    Location:87 → 274
    FAD_binding_3; FAD binding domain
    cd21250
    Location:514 → 623
    CH_MICAL2; calponin homology (CH) domain found in molecule interacting with CasL protein 2

  6. XM_030242685.1 → XP_030098545.1  F-actin-monooxygenase MICAL2 isoform X6

    Conserved Domains (3) summary
    cd09439
    Location:753 → 807
    LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)
    pfam01494
    Location:87 → 274
    FAD_binding_3; FAD binding domain
    cd21250
    Location:514 → 623
    CH_MICAL2; calponin homology (CH) domain found in molecule interacting with CasL protein 2

  7. XM_036153175.1 → XP_036009068.1  F-actin-monooxygenase MICAL2 isoform X6

    Conserved Domains (3) summary
    cd09439
    Location:753 → 807
    LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)
    pfam01494
    Location:87 → 274
    FAD_binding_3; FAD binding domain
    cd21250
    Location:514 → 623
    CH_MICAL2; calponin homology (CH) domain found in molecule interacting with CasL protein 2

RNA

  1. XR_001785575.2 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8BML1.2 GenPept UniProtKB/Swiss-Prot:Q8BML1

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