U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

MED4 mediator complex subunit 4 [ Homo sapiens (human) ]

Gene ID: 29079, updated on 5-Mar-2024

Summary

Go to the top of the page Help
Official Symbol
MED4provided by HGNC
Official Full Name
mediator complex subunit 4provided by HGNC
Primary source
HGNC:HGNC:17903
See related
Ensembl:ENSG00000136146 MIM:605718; AllianceGenome:HGNC:17903
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
ARC36; VDRIP; DRIP36; TRAP36; HSPC126
Summary
This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
Expression
Ubiquitous expression in testis (RPKM 13.4), bone marrow (RPKM 12.6) and 25 other tissues See more
Orthologs
mouse all
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Location:
13q14.2
Exon count:
7
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 13 NC_000013.11 (48075724..48095104, complement)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 13 NC_060937.1 (47296104..47315464, complement)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 13 NC_000013.10 (48649860..48669240, complement)

Chromosome 13 - NC_000013.11Genomic Context describing neighboring genes Neighboring gene succinate-CoA ligase ADP-forming subunit beta Neighboring gene ATAC-STARR-seq lymphoblastoid active region 7717 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 7718 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 7719 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5330 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr13:48575750-48576949 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr13:48577621-48578218 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr13:48578817-48579414 Neighboring gene uncharacterized LOC124903172 Neighboring gene OCT4-NANOG-H3K27ac hESC enhancer GRCh37_chr13:48610990-48611866 Neighboring gene OCT4-NANOG-H3K27ac hESC enhancer GRCh37_chr13:48611867-48612742 Neighboring gene nudix hydrolase 15 Neighboring gene MED14-independent group 3 enhancer GRCh37_chr13:48640162-48641361 Neighboring gene MED14-independent group 3 enhancer GRCh37_chr13:48646101-48647300 Neighboring gene Sharpr-MPRA regulatory region 5997 Neighboring gene MED4 antisense RNA 1 Neighboring gene uncharacterized LOC105370198 Neighboring gene RNA polymerase II subunit K pseudogene 2 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 7723 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr13:48733841-48734451 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 7724 Neighboring gene Sharpr-MPRA regulatory region 1366 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5331 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5332 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr13:48807853-48808550 Neighboring gene OCT4-NANOG hESC enhancer GRCh37_chr13:48809655-48810242 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr13:48815437-48815937 Neighboring gene integral membrane protein 2B

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help
  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Genotype-phenotype correlations in patients with retinoblastoma and interstitial 13q deletions. Mitter D, et al. Eur J Hum Genet, 2011 Sep. PMID 21505449, Free PMC Article
  2. The mediator of RNA polymerase II. Blazek E, et al. Chromosoma, 2005 Mar. PMID 15690163
  3. The mammalian Mediator complex. Conaway JW, et al. FEBS Lett, 2005 Feb 7. PMID 15680972
  4. The survival gene MED4 explains low penetrance retinoblastoma in patients with large RB1 deletion. Dehainault C, et al. Hum Mol Genet, 2014 Oct 1. PMID 24858910
  5. Gene dosage, expression, and ontology analysis identifies driver genes in the carcinogenesis and chemoradioresistance of cervical cancer. Lando M, et al. PLoS Genet, 2009 Nov. PMID 19911042, Free PMC Article

See all (104) citations in PubMed

GeneRIFs: Gene References Into Functions

Phenotypes

Go to the top of the page Help

Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

Go to the top of the page Help

See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

Go to the top of the page Help

Replication interactions

Interaction Pubs
Knockdown of mediator complex subunit 4 (MED4) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells PubMed
Knockdown of mediator complex subunit 4 (MED4) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Tat tat Knockdown of MED4 by siRNAs inhibits HIV LTR-beta-gal activation in the Tat transactivation assay PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Markers

Homology

Clone Names

  • FLJ10956

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables nuclear receptor coactivator activity NAS
Non-traceable Author Statement
more info
 PubMed 
enables nuclear thyroid hormone receptor binding IDA
Inferred from Direct Assay
more info
  
enables nuclear vitamin D receptor binding NAS
Non-traceable Author Statement
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transcription coactivator activity IDA
Inferred from Direct Assay
more info
  
enables transcription coregulator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables transcription coregulator activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in RNA polymerase II preinitiation complex assembly NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in positive regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
  
involved_in positive regulation of transcription elongation by RNA polymerase II NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in positive regulation of transcription initiation by RNA polymerase II IDA
Inferred from Direct Assay
more info
  
involved_in positive regulation of transcription initiation by RNA polymerase II NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of core mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of core mediator complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of mediator complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane HDA PubMed 
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  
located_in nucleoplasm TAS
Traceable Author Statement
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
mediator of RNA polymerase II transcription subunit 4
Names
activator-recruited cofactor 36 kDa component
mediator, 34-kD subunit, homolog
vitamin D receptor-interacting protein, 36-kD
vitamin D3 receptor-interacting protein complex 36 kDa component

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001270629.2 → NP_001257558.1  mediator of RNA polymerase II transcription subunit 4 isoform 2

    See identical proteins and their annotated locations for NP_001257558.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses an alternate 5' exon structure and initiates translation at a downstream in-frame start codon compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1.
    Source sequence(s)
    AI613470, AK301835, AL158196
    Consensus CDS
    CCDS59241.1
    UniProtKB/TrEMBL
    Q5T911
    Related
    ENSP00000367849.1, ENST00000378586.5
    Conserved Domains (1) summary
    pfam10018
    Location:18 → 143
    Med4; Vitamin-D-receptor interacting Mediator subunit 4

  2. NM_014166.4 → NP_054885.1  mediator of RNA polymerase II transcription subunit 4 isoform 1

    See identical proteins and their annotated locations for NP_054885.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AI613470, AK001934, AL158196
    Consensus CDS
    CCDS9408.1
    UniProtKB/Swiss-Prot
    B4DX67, Q53GB4, Q53H68, Q5T912, Q6FHC4, Q6IA79, Q9BS95, Q9NPJ6, Q9NYR5
    UniProtKB/TrEMBL
    U4PSC4
    Related
    ENSP00000258648.2, ENST00000258648.7
    Conserved Domains (1) summary
    pfam10018
    Location:64 → 189
    Med4; Vitamin-D-receptor interacting Mediator subunit 4

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000013.11 Reference GRCh38.p14 Primary Assembly

    Range
    48075724..48095104 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060937.1 Alternate T2T-CHM13v2.0

    Range
    47296104..47315464 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9NPJ6.1 GenPept UniProtKB/Swiss-Prot:Q9NPJ6

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Chemical Information
Molecular Biology Databases
Research Materials
Tools
Miscellaneous