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Pfkfb4 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 [ Mus musculus (house mouse) ]

Gene ID: 270198, updated on 11-Apr-2024

Summary

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Official Symbol
Pfkfb4provided by MGI
Official Full Name
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4provided by MGI
Primary source
MGI:MGI:2687284
See related
Ensembl:ENSMUSG00000025648 AllianceGenome:MGI:2687284
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
C230090D14
Summary
Predicted to enable 6-phosphofructo-2-kinase activity and fructose-2,6-bisphosphate 2-phosphatase activity. Predicted to be involved in fructose 2,6-bisphosphate metabolic process. Predicted to act upstream of or within phosphorylation. Predicted to be active in cytosol. Is expressed in several structures, including eye; genitourinary system; gut; nervous system; and respiratory system. Orthologous to human PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in large intestine adult (RPKM 18.1), small intestine adult (RPKM 17.4) and 25 other tissues See more
Orthologs
human all
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Genomic context

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See Pfkfb4 in Genome Data Viewer
Location:
9 F2; 9 59.63 cM
Exon count:
18
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 9 NC_000075.7 (108817336..108861302)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 9 NC_000075.6 (108988272..109032236)

Chromosome 9 - NC_000075.7Genomic Context describing neighboring genes Neighboring gene collagen, type VII, alpha 1 Neighboring gene microRNA 711 Neighboring gene STARR-positive B cell enhancer ABC_E192 Neighboring gene urocortin 2 Neighboring gene STARR-seq mESC enhancer starr_25229 Neighboring gene STARR-positive B cell enhancer ABC_E5105 Neighboring gene shisa family member 5 Neighboring gene STARR-positive B cell enhancer ABC_E5106 Neighboring gene STARR-positive B cell enhancer ABC_E10586 Neighboring gene STARR-positive B cell enhancer ABC_E10587 Neighboring gene STARR-positive B cell enhancer ABC_E5107 Neighboring gene predicted gene, 53191 Neighboring gene three prime repair exonuclease 1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Hypoxic activation of PFKFB4 in breast tumor microenvironment shapes metabolic and cellular plasticity to accentuate metastatic competence. Dai T, et al. Cell Rep, 2022 Dec 6. PMID 36476868, Free PMC Article
  2. PFKFB4 facilitates palbociclib resistance in oestrogen receptor-positive breast cancer by enhancing stemness. Wang S, et al. Cell Prolif, 2023 Jan. PMID 36127291, Free PMC Article
  3. Targeting the sugar metabolism of tumors with a first-in-class 6-phosphofructo-2-kinase (PFKFB4) inhibitor. Chesney J, et al. Oncotarget, 2015 Jul 20. PMID 26221874, Free PMC Article
  4. Splice isoform of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-4: expression and hypoxic regulation. Minchenko OH, et al. Mol Cell Biochem, 2005 Dec. PMID 16311927
  5. Hypoxic regulation of the 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene family (PFKFB-1-4) expression in vivo. Minchenko O, et al. FEBS Lett, 2003 Nov 20. PMID 14623077

See all (23) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. PFKFB4 facilitates palbociclib resistance in oestrogen receptor-positive breast cancer by enhancing stemness.
    Title: PFKFB4 facilitates palbociclib resistance in oestrogen receptor-positive breast cancer by enhancing stemness.
  2. Hypoxic activation of PFKFB4 in breast tumor microenvironment shapes metabolic and cellular plasticity to accentuate metastatic competence.
    Title: Hypoxic activation of PFKFB4 in breast tumor microenvironment shapes metabolic and cellular plasticity to accentuate metastatic competence.
  3. Inhibition of PFKFB4 suppresses glycolysis and proliferation of multiple human cancer cell lines.
    Title: Targeting the sugar metabolism of tumors with a first-in-class 6-phosphofructo-2-kinase (PFKFB4) inhibitor.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (2) 
  • Endonuclease-mediated (3) 

Pathways from PubChem

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General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables 6-phosphofructo-2-kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 6-phosphofructo-2-kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables fructose-2,6-bisphosphate 2-phosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables fructose-2,6-bisphosphate 2-phosphatase activity ISO
Inferred from Sequence Orthology
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in fructose 2,6-bisphosphate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in fructose metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within phosphorylation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
Names
6PF-2-K/Fru-2,6-P2ase 4
6PF-2-K/Fru-2,6-P2ase testis-type isozyme
PFK/FBPase 4
NP_766607.3
XP_006512196.1
XP_017168891.1
XP_017168892.1
XP_017168893.1
XP_030100264.1
XP_036010927.1
XP_036010928.1
XP_036010929.1
XP_036010930.1
XP_036010931.1
XP_036010932.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_173019.5 → NP_766607.3  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4

    See identical proteins and their annotated locations for NP_766607.3

    Status: VALIDATED

    Source sequence(s)
    AY646092, BC057594, CA316033
    Consensus CDS
    CCDS23541.1
    UniProtKB/Swiss-Prot
    Q5UD55, Q5UD56, Q5UD57, Q5Y296, Q6DTY7, Q6PFE8
    Related
    ENSMUSP00000142378.2, ENSMUST00000198140.5
    Conserved Domains (2) summary
    pfam01591
    Location:35 → 249
    6PF2K; 6-phosphofructo-2-kinase
    pfam00300
    Location:252 → 435
    His_Phos_1; Histidine phosphatase superfamily (branch 1)

RNA

  1. NR_153429.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AY707862, BC057594, CA316033

  2. NR_153430.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AY756065, BC057594, CA316033

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000075.7 Reference GRCm39 C57BL/6J

    Range
    108817336..108861302
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036155037.1 → XP_036010930.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X4

    Conserved Domains (2) summary
    pfam00300
    Location:305 → 477
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:24 → 302
    6PF2K; 6-phosphofructo-2-kinase

  2. XM_036155035.1 → XP_036010928.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X2

    Conserved Domains (2) summary
    pfam00300
    Location:308 → 480
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:27 → 305
    6PF2K; 6-phosphofructo-2-kinase

  3. XM_036155038.1 → XP_036010931.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X5

    Conserved Domains (2) summary
    pfam00300
    Location:294 → 466
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:13 → 291
    6PF2K; 6-phosphofructo-2-kinase

  4. XM_006512133.3 → XP_006512196.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X6

    Conserved Domains (2) summary
    pfam01591
    Location:35 → 249
    6PF2K; 6-phosphofructo-2-kinase
    pfam00300
    Location:252 → 431
    His_Phos_1; Histidine phosphatase superfamily (branch 1)

  5. XM_036155036.1 → XP_036010929.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X3

    Conserved Domains (2) summary
    pfam00300
    Location:316 → 502
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:30 → 313
    6PF2K; 6-phosphofructo-2-kinase

  6. XM_036155034.1 → XP_036010927.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X1

    Conserved Domains (2) summary
    pfam00300
    Location:316 → 488
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:30 → 313
    6PF2K; 6-phosphofructo-2-kinase

  7. XM_030244404.2 → XP_030100264.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X7

    Conserved Domains (2) summary
    pfam01591
    Location:1 → 215
    6PF2K; 6-phosphofructo-2-kinase
    pfam00300
    Location:218 → 397
    His_Phos_1; Histidine phosphatase superfamily (branch 1)

  8. XM_017313402.3 → XP_017168891.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X8

    UniProtKB/TrEMBL
    C9VZF2
    Conserved Domains (2) summary
    pfam01591
    Location:1 → 215
    6PF2K; 6-phosphofructo-2-kinase
    pfam00300
    Location:218 → 401
    His_Phos_1; Histidine phosphatase superfamily (branch 1)

  9. XM_017313404.2 → XP_017168893.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X10

    UniProtKB/TrEMBL
    C9VZF3
    Conserved Domains (2) summary
    pfam00300
    Location:61 → 244
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    cl17190
    Location:1 → 58
    NK; Nucleoside/nucleotide kinase (NK) is a protein superfamily consisting of multiple families of enzymes that share structural similarity and are functionally related to the catalysis of the reversible phosphate group transfer from nucleoside triphosphates ...

  10. XM_036155039.1 → XP_036010932.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X11

    Conserved Domains (2) summary
    pfam00300
    Location:34 → 206
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    cl17190
    Location:1 → 31
    NK; Nucleoside/nucleotide kinase (NK) is a protein superfamily consisting of multiple families of enzymes that share structural similarity and are functionally related to the catalysis of the reversible phosphate group transfer from nucleoside triphosphates ...

  11. XM_017313403.2 → XP_017168892.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X9

    Conserved Domains (2) summary
    pfam00300
    Location:61 → 240
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    cl17190
    Location:1 → 58
    NK; Nucleoside/nucleotide kinase (NK) is a protein superfamily consisting of multiple families of enzymes that share structural similarity and are functionally related to the catalysis of the reversible phosphate group transfer from nucleoside triphosphates ...

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_001039215.1: Suppressed sequence

    Description
    NM_001039215.1: This RefSeq was permanently suppressed because it is a nonsense-mediated mRNA decay (NMD) candidate.

  2. NM_001039216.1: Suppressed sequence

    Description
    NM_001039216.1: This RefSeq was permanently suppressed because it is a nonsense-mediated mRNA decay (NMD) candidate.

  3. NM_001039217.1: Suppressed sequence

    Description
    NM_001039217.1: This RefSeq was removed because currently there is support for the transcript but not for the protein.
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q6DTY7.4 GenPept UniProtKB/Swiss-Prot:Q6DTY7

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