Lpcat2 lysophosphatidylcholine acyltransferase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Lpcat2provided by MGI
Official Full Name: lysophosphatidylcholine acyltransferase 2provided by MGI
Primary source: MGI:MGI:3606214
See related: Ensembl:ENSMUSG00000033192 AllianceGenome:MGI:3606214
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Aytl1; Aytl1a; lpafat1; lysoPAFAT; 1-AGPAT 11; A330042H22; lysoPAFAT/LPCAT2
Summary: Enables 1-acylglycerophosphocholine O-acyltransferase activity and 1-alkylglycerophosphocholine O-acetyltransferase activity. Involved in membrane organization and platelet activating factor biosynthetic process. Located in Golgi stack; endoplasmic reticulum; and membrane. Orthologous to human LPCAT2 (lysophosphatidylcholine acyltransferase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in spleen adult (RPKM 4.2), colon adult (RPKM 3.9) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 C5; 8 44.99 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (93581948..93645907)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (92855313..92919279)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Possible regulation of Toll-like receptor 4 by lysine acetylation through LPCAT2 activity in RAW264.7 cells.
Title: Possible regulation of Toll-like receptor 4 by lysine acetylation through LPCAT2 activity in RAW264.7 cells.
ROBO4 deletion ameliorates PAF-mediated skin inflammation via regulating the mRNA translation efficiency of LPCAT1/LPCAT2 and the expression of PAF receptor.
Title: ROBO4 deletion ameliorates PAF-mediated skin inflammation via regulating the mRNA translation efficiency of LPCAT1/LPCAT2 and the expression of PAF receptor.
Lysophosphatidylcholine acyltransferase 2 (LPCAT2) co-localises with TLR4 and regulates macrophage inflammatory gene expression in response to LPS.
Title: Lysophosphatidylcholine acyltransferase 2 (LPCAT2) co-localises with TLR4 and regulates macrophage inflammatory gene expression in response to LPS.
Selected compounds competed with acetyl-CoA for the inhibition of LPCAT2 lyso-PAFAT activity and suppressed PAF biosynthesis in mouse peritoneal macrophages stimulated with a calcium ionophore. These compounds had low inhibitory effects on LPCAT1 activity, indicating that adverse effects on respiratory functions may be avoided.
Title: Selective inhibitors of a PAF biosynthetic enzyme lysophosphatidylcholine acyltransferase 2.
LPCAT2 was detected in wild-type spinal cord microglia, and the absence of LPCAT2 expression precluded spinal platelet-activating factor expression in LPCAT2-knockout mice.
Title: Relief from neuropathic pain by blocking of the platelet-activating factor-pain loop.
We show a new signaling pathway for rapid biosynthesis of PAF that is mediated by phosphorylation of LPCAT2 at Ser-34.
Title: Rapid production of platelet-activating factor is induced by protein kinase Cα-mediated phosphorylation of lysophosphatidylcholine acyltransferase 2 protein.
Specific inhibition of the PAF biosynthetic activity by phosphorylated LPCAT2 will provide a novel target for the regulation of inflammatory disorders.
Title: Phosphorylation of lysophosphatidylcholine acyltransferase 2 at Ser34 enhances platelet-activating factor production in endotoxin-stimulated macrophages.
Cloned the mouse acetyl-CoA:LYSO-PAF acetyltransferase and found that the enzyme catalyzed not only biosynthesis of PAF from lyso-PAF but also incorporation of arachidonoyl-CoA to produce PAF precursor membrane glycerophospholipids.
Title: A single enzyme catalyzes both platelet-activating factor production and membrane biogenesis of inflammatory cells. Cloning and characterization of acetyl-CoA:LYSO-PAF acetyltransferase.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Endonuclease-mediated (6)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 1-acylglycerol-3-phosphate O-acyltransferase activity	ISO Inferred from Sequence Orthology more info
enables 1-acylglycerophosphocholine O-acyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables 1-acylglycerophosphocholine O-acyltransferase activity	ISO Inferred from Sequence Orthology more info
enables 1-alkenylglycerophosphocholine O-acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables 1-alkylglycerophosphocholine O-acetyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables O-acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables lysophosphatidic acid acyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables plasmalogen synthase activity	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in membrane organization	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphatidylcholine acyl-chain remodeling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phospholipid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in platelet activating factor biosynthetic process	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi stack	IDA Inferred from Direct Assay more info	PubMed
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
located_in lipid droplet	ISO Inferred from Sequence Orthology more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: lysophosphatidylcholine acyltransferase 2

Names: 1-AGP acyltransferase 11; 1-acylglycerol-3-phosphate O-acyltransferase 11; 1-acylglycerophosphocholine O-acyltransferase; 1-alkenylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; LPC acyltransferase 2; acetyl-CoA:lyso-PAF acetyltransferase; acetyl-CoA:lyso-platelet-activating factor acetyltransferase; acyl-CoA:lysophosphatidylcholine acyltransferase member 1; acyltransferase like 1A; lyso-PAF acetyltransferase; lysoPC acyltransferase 2

NP_001344303.1

EC 2.3.1.23

EC 2.3.1.25

EC 2.3.1.51

EC 2.3.1.67

NP_001344304.1

EC 2.3.1.23

EC 2.3.1.25

EC 2.3.1.51

EC 2.3.1.67

NP_766602.1

EC 2.3.1.23

EC 2.3.1.25

EC 2.3.1.51

EC 2.3.1.67

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001357374.1 → NP_001344303.1 lysophosphatidylcholine acyltransferase 2 isoform b

Status: VALIDATED

Source sequence(s)

AC121604

Consensus CDS

CCDS90434.1

UniProtKB/TrEMBL

B2RWR2

Related

ENSMUSP00000147941.2, ENSMUST00000210099.2

Conserved Domains (2) summary

cd07991
Location:115 → 287

LPLAT_LPCAT1-like; Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: LPCAT1-like

pfam13499
Location:354 → 418

EF-hand_7; EF-hand domain pair
NM_001357375.1 → NP_001344304.1 lysophosphatidylcholine acyltransferase 2 isoform c

Status: VALIDATED

Source sequence(s)

AC121604

Conserved Domains (5) summary

cd00051
Location:165 → 227

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam13499
Location:202 → 259

EF-hand_7; EF-hand domain pair

cd15897
Location:124 → 154

EFh_PEF; EF-hand motif [structural motif]

cl17185
Location:3 → 96

LPLAT; Lysophospholipid acyltransferases (LPLATs) of glycerophospholipid biosynthesis

cl25352
Location:93 → 217

EFh_PEF; The penta-EF hand (PEF) family
NM_173014.2 → NP_766602.1 lysophosphatidylcholine acyltransferase 2 isoform a

See identical proteins and their annotated locations for NP_766602.1

Status: VALIDATED

Source sequence(s)

AC121604, AK039431

Consensus CDS

CCDS22524.1

UniProtKB/Swiss-Prot

A3KBM0, A9Q1G2, A9Q1G3, Q8BYI6

UniProtKB/TrEMBL

B2RWR2

Related

ENSMUSP00000049252.2, ENSMUST00000046290.3

Conserved Domains (5) summary

cd07991
Location:115 → 327

LPLAT_LPCAT1-like; Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: LPCAT1-like

COG0204
Location:69 → 254

PlsC; 1-acyl-sn-glycerol-3-phosphate acyltransferase [Lipid transport and metabolism]

cd00051
Location:396 → 458

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam13499
Location:396 → 457

EF-hand_7; EF-hand domain pair

cl08302
Location:433 → 486

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...