Mpg N-methylpurine-DNA glycosylase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mpgprovided by MGI
Official Full Name: N-methylpurine-DNA glycosylaseprovided by MGI
Primary source: MGI:MGI:97073
See related: Ensembl:ENSMUSG00000020287 AllianceGenome:MGI:97073
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Aag; APNG; Mid1; 9830006D05
Summary: Enables alkylbase DNA N-glycosylase activity. Acts upstream of or within base-excision repair. Predicted to be located in cytosol and nucleoplasm. Is expressed in several structures, including early conceptus; gonad; hemolymphoid system gland; musculature; and placenta. Orthologous to human MPG (N-methylpurine DNA glycosylase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in adrenal adult (RPKM 8.1), genital fat pad adult (RPKM 7.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 A4; 11 18.83 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (32176505..32182702)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (32226505..32232702)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Alkyladenine DNA glycosylase deficiency uncouples alkylation-induced strand break generation from PARP-1 activation and glycolysis inhibition.
Title: Alkyladenine DNA glycosylase deficiency uncouples alkylation-induced strand break generation from PARP-1 activation and glycolysis inhibition.
ZEB1 promotes colitis and inflammatory CRC through the inhibition of MPG in epithelial cells, thus offering new therapeutic strategies to modulate inflammation and inflammatory cancer
Title: ZEB1 promotes inflammation and progression towards inflammation-driven carcinoma through repression of the DNA repair glycosylase MPG in epithelial cells.
The depletion of Aag did not significantly change the transcriptional inhibitory or mutagenic properties of all five examined lesions, suggesting a negligible role of Aag in the repair of these DNA adducts in mammalian cells.
Title: Roles of Aag, Alkbh2, and Alkbh3 in the Repair of Carboxymethylated and Ethylated Thymidine Lesions.
This study demonstrates for the first time a non-linear dose-response for alkylation-induced colorectal carcinogenesis and reveals DNA repair by MGMT, but not AAG, as a key node in determining a carcinogenic threshold.
Title: DNA repair by MGMT, but not AAG, causes a threshold in alkylation-induced colorectal carcinogenesis.
the detrimental effects of Aag-initiated BER during I/R and sterile inflammation, and present a novel target for controlling I/R-induced injury.
Title: Aag-initiated base excision repair promotes ischemia reperfusion injury in liver, brain, and kidney.
Toxicity, induced by tert-butyl-hydroperoxide and potassium bromate, differs in base excision repair proficient (Mpg (+/+), Nth1 (+/+)) and deficient (Mpg (-/-), Nth1 (-/-)) mouse embryonic fibroblasts following Msh2 knockdown, was examined.
Title: The effect of Msh2 knockdown on toxicity induced by tert-butyl-hydroperoxide, potassium bromate, and hydrogen peroxide in base excision repair proficient and deficient cells.
These results provide in vivo evidence that Aag-initiated BER may play a critical role in determining the side-effects of alkylating agent chemotherapies and that Parp1 plays a crucial role in Aag-mediated tissue damage.
Title: Aag DNA glycosylase promotes alkylation-induced tissue damage mediated by Parp1.
ALKBH2 and ALKBH3 provide cancer protection similar to that of the DNA glycosylase AAG and display apparent epistasis with Aag
Title: DNA repair is indispensable for survival after acute inflammation.
RNS-induced posttranslational modification of AAG is one mechanism of base excision repair dysregulation
Title: Differential effects of reactive nitrogen species on DNA base excision repair initiated by the alkyladenine DNA glycosylase.
Clock gene mutation modulates the cellular sensitivity to genotoxic stress through altering the expression of Mpg gene.
Title: Clock gene mutation modulates the cellular sensitivity to genotoxic stress through altering the expression of N-methylpurine DNA glycosylase gene.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation

General gene information

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Markers

Mpg (e-PCR), detects polymorphism
- UniSTS:143903

Mpg (e-PCR), detects polymorphism
- UniSTS:238151

AI326268 (e-PCR)
- UniSTS:180172

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-3-methyladenine glycosylase activity	IEA Inferred from Electronic Annotation more info
enables DNA-3-methylguanine glycosylase activity	IEA Inferred from Electronic Annotation more info
enables DNA-7-methyladenine glycosylase activity	IEA Inferred from Electronic Annotation more info
enables DNA-7-methylguanine glycosylase activity	IEA Inferred from Electronic Annotation more info
enables alkylbase DNA N-glycosylase activity	IBA Inferred from Biological aspect of Ancestor more info
enables alkylbase DNA N-glycosylase activity	IDA Inferred from Direct Assay more info	PubMed
enables alkylbase DNA N-glycosylase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA repair	IEA Inferred from Electronic Annotation more info
involved_in base-excision repair	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within base-excision repair	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in mitochondrial nucleoid	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-3-methyladenine glycosylase

Names: 3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; 3-methyladenine DNA glycosylase; ADPG; alkylpurine-DNA-N-glycosylase

NP_034952.2

EC 3.2.2.21

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.