Myrip myosin VIIA and Rab interacting protein [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Myripprovided by MGI
Official Full Name: myosin VIIA and Rab interacting proteinprovided by MGI
Primary source: MGI:MGI:2384407
See related: Ensembl:ENSMUSG00000041794 AllianceGenome:MGI:2384407
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Slac2c; Slac2-c; A230081N12Rik
Summary: Enables cytoskeletal protein binding activity; protein kinase A binding activity; and small GTPase binding activity. Predicted to be involved in positive regulation of insulin secretion. Predicted to act upstream of or within vesicle transport along actin filament. Located in several cellular components, including actin cytoskeleton; melanosome; and photoreceptor outer segment. Part of exocyst. Is expressed in several structures, including gut; heart; lung; male reproductive gland or organ; and nervous system. Orthologous to human MYRIP (myosin VIIA and Rab interacting protein). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in cortex adult (RPKM 6.5), frontal lobe adult (RPKM 4.4) and 16 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 F4; 9 71.5 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (120132996..120305167)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (120303520..120476101)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Here, the authors show that exophilin-8 accumulates granules in the cortical F-actin network not by direct interaction with myosin-Va, but by indirect interaction with a specific form of myosin-VIIa through its previously unknown binding partner, RIM-BP2.
Title: Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa.
Exophilin8 transiently clusters insulin granules at the actin-rich cell cortex prior to exocytosis
Title: Exophilin8 transiently clusters insulin granules at the actin-rich cell cortex prior to exocytosis.
Myrip is a versatile Rab27a-associated myosin-activating protein that mediates cellular activation of MyoVa and MyoVIIa via non-overlapping domains.
Title: Myrip uses distinct domains in the cellular activation of myosin VA and myosin VIIA in melanosome transport.
Results suggest that the Rab27a-Myrip-MyoVIIa complex regulates tethering of melanosomes onto actin filaments, a process that ensures melanosome movement towards the cell periphery.
Title: The ternary Rab27a-Myrip-Myosin VIIa complex regulates melanosome motility in the retinal pigment epithelium.
indicate that MyRIP functions as a scaffolding protein that links protein kinase A to components of the exocytosis machinery
Title: MyRIP anchors protein kinase A to the exocyst complex.
The present results provide evidence from live retinal pigment epithelium cells that the RAB27A-MYRIP-MYO7A complex functions in melanosome motility.
Title: Analysis of the linkage of MYRIP and MYO7A to melanosomes by RAB27A in retinal pigment epithelial cells.
Synaptotagmin-like protein homologue lacking C2 domains-c) is a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin
Title: Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin.
Slac2-c acts as a functional myosin VIIa receptor and the Rab27A.Slac2-c x myosin VIIa tripartite protein complex regulates the transport of retinal melanosomes in pigment epithelium cells
Title: Functional analysis of Slac2-c/MyRIP as a linker protein between melanosomes and myosin VIIa.
Slac2-c/MyRIP contains an N-terminal Slp homology domain (SHD) and functions as a linker protein that interacts with myosin Rab27A/B, VIIa/Va, and actin.
Title: Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (1)

General gene information

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Markers

20.MMHAP29FRG8.seq (e-PCR)
- UniSTS:123467

Myrip (e-PCR), detects polymorphism
- UniSTS:259188

D9Mit19 (e-PCR), detects polymorphism
- UniSTS:131747

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables actin binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables myosin binding	IBA Inferred from Biological aspect of Ancestor more info
enables myosin binding	IDA Inferred from Direct Assay more info	PubMed
enables myosin binding	ISO Inferred from Sequence Orthology more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase A binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase A binding	ISO Inferred from Sequence Orthology more info
enables small GTPase binding	IDA Inferred from Direct Assay more info	PubMed
enables small GTPase binding	ISO Inferred from Sequence Orthology more info	PubMed

Process	Evidence Code	Pubs
involved_in intracellular protein transport	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of insulin secretion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within vesicle transport along actin filament	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in cortical actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in dense core granule	ISO Inferred from Sequence Orthology more info
part_of exocyst	IDA Inferred from Direct Assay more info	PubMed
located_in melanosome	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in photoreceptor outer segment	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: rab effector MyRIP

Names: exophilin 8; myosin-VIIa- and Rab-interacting protein; slp homolog lacking C2 domains c; synaptotagmin-like protein lacking C2 domains C

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_144557.5 → NP_653140.1 rab effector MyRIP

See identical proteins and their annotated locations for NP_653140.1

Status: PROVISIONAL

Source sequence(s)

AC123708, AC167243

Consensus CDS

CCDS23627.1

UniProtKB/Swiss-Prot

A1L320, A1L321, Q8CFC0, Q8K3I4, Q8K4H5

Related

ENSMUSP00000046891.7, ENSMUST00000048121.13

Conserved Domains (2) summary

pfam02318
Location:8 → 125

FYVE_2; FYVE-type zinc finger

pfam04698
Location:153 → 856

Rab_eff_C; Rab effector MyRIP/melanophilin C-terminus

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

120132996..120305167

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006512128.5 → XP_006512191.1 rab effector MyRIP isoform X3

UniProtKB/TrEMBL

A0A1L1SQQ9

Conserved Domains (2) summary

pfam02318
Location:8 → 125

FYVE_2; FYVE-type zinc finger

pfam04698
Location:153 → 633

Rab_eff_C; Rab effector MyRIP/melanophilin C-terminus
XM_006512125.4 → XP_006512188.1 rab effector MyRIP isoform X1

See identical proteins and their annotated locations for XP_006512188.1

UniProtKB/Swiss-Prot

A1L320, A1L321, Q8CFC0, Q8K3I4, Q8K4H5

Conserved Domains (2) summary

pfam02318
Location:8 → 125

FYVE_2; FYVE-type zinc finger

pfam04698
Location:153 → 856

Rab_eff_C; Rab effector MyRIP/melanophilin C-terminus
XM_006512127.5 → XP_006512190.1 rab effector MyRIP isoform X2

Conserved Domains (2) summary

cl28890
Location:10 → 36

FYVE_like_SF; FYVE domain like superfamily

pfam04698
Location:52 → 755

Rab_eff_C; Rab effector MyRIP/melanophilin C-terminus