Prickle2 prickle planar cell polarity protein 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prickle2provided by MGI
Official Full Name: prickle planar cell polarity protein 2provided by MGI
Primary source: MGI:MGI:1925144
See related: Ensembl:ENSMUSG00000030020 AllianceGenome:MGI:1925144
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pk2; mpk2; 6230400G14Rik; 6720451F06Rik
Summary: Predicted to enable zinc ion binding activity. Acts upstream of or within several processes, including establishment or maintenance of epithelial cell apical/basal polarity; neuron projection development; and trophectodermal cell fate commitment. Located in several cellular components, including apicolateral plasma membrane; lateral plasma membrane; and nucleus. Is expressed in several structures, including brain; genitourinary system; hemolymphoid system gland; notochord; and primitive streak. Orthologous to human PRICKLE2 (prickle planar cell polarity protein 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in cortex adult (RPKM 12.7), frontal lobe adult (RPKM 12.2) and 19 other tissues See more
Orthologs: human all
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Genomic context

Location:: 6 D1; 6 41.05 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (92347876..92683233, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (92370891..92706334, complement)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Exogenously introduced expression of green fluorescent protein (GFP)-tagged core PCP factors by electroporation revealed that Vangl1, Vangl2 and Prickle2 are localized on the ovarian side of the cell periphery in the oviduct.Our data collectively suggest that the mechanism for maintenance of Vangl2 asymmetry in mature mouse oviduct is different from the microtubule dependent polarized transport model
Title: Dynamics of planar cell polarity protein Vangl2 in the mouse oviduct epithelium.
The results of this study demonstrated that Prickle2 is required for normal ependymal motile cilia development and function.
Title: Defective motile cilia in Prickle2-deficient mice.
Prickle2 disruption causes behavioral abnormalities like altered social interaction, learning abnormalities and behavioral inflexibility. In mouse hippocampal neurons, it reduced dendrite branching, synapse number and postsynaptic density size.
Title: Disruption of the non-canonical Wnt gene PRICKLE2 leads to autism-like behaviors with evidence for hippocampal synaptic dysfunction.
The cell polarity phenotype was efficiently rescued by nuclear but not cytoplasmic Prickle2, demonstrating the nuclear localization of Prickle2 is critical for its function.
Title: Nuclear localization of Prickle2 is required to establish cell polarity during early mouse embryogenesis.
Prickle1 and Prickle2 promote neurite-like process formation of C1300 cells via the Dvl1-dependent mechanism
Title: Role of Prickle1 and Prickle2 in neurite outgrowth in murine neuroblastoma cells.
The prickle2 protein is a novel PSD protein that interacts with PSD-95 and NMDA receptors through complex formations in the brain.
Title: Prickle2 is localized in the postsynaptic density and interacts with PSD-95 and NMDA receptors in the brain.
both Prickle1 and Prickle2 promote neurite-like process formation of C1300 neuroblastoma cells via the Dishevelled dependent pathway
Title: Prickle promotes neurite outgrowth via the Dishevelled dependent pathway in C1300 cells.
mouse Prickle1 and Prickle2 possibly regulate positive neurite formation during brain development
Title: Mouse Prickle1 and Prickle2 are expressed in postmitotic neurons and promote neurite outgrowth.
Putative planar cell polarity protein prickle-like 2 is distributed in crescents on the medial sides of vestibular epithelial cells before the morphological polarization of hair cells.
Title: Asymmetric distribution of prickle-like 2 reveals an early underlying polarization of vestibular sensory epithelia in the inner ear.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D6Mit284 (e-PCR), detects polymorphism
- UniSTS:130831

STS-R99346 (e-PCR)
- UniSTS:65406

AI385205 (e-PCR)
- UniSTS:226218

Prickle2 (e-PCR), detects polymorphism
- UniSTS:532462

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in embryonic brain development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within establishment or maintenance of epithelial cell apical/basal polarity	IDA Inferred from Direct Assay more info	PubMed
involved_in maintenance of postsynaptic density structure	IDA Inferred from Direct Assay more info	PubMed
involved_in maintenance of postsynaptic density structure	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neuron projection development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron projection development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of postsynaptic density assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynaptic density assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to electrical stimulus	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in apicolateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in lateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic density	IEP Inferred from Expression Pattern more info	PubMed

General protein information

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Preferred Names: prickle-like protein 2

Names: prickle homolog 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001081146.3 → NP_001074615.1 prickle-like protein 2 isoform a

See identical proteins and their annotated locations for NP_001074615.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes isoform a.

Source sequence(s)

AC164636, AC166101

Consensus CDS

CCDS39572.1

UniProtKB/TrEMBL

A7YQ68

Related

ENSMUSP00000032093.6, ENSMUST00000032093.12

Conserved Domains (4) summary

cd09415
Location:186 → 244

LIM1_Prickle; The first LIM domain of Prickle

cd09418
Location:249 → 304

LIM2_Prickle; The second LIM domain of Prickle

cd09420
Location:309 → 367

LIM3_Prickle; The third LIM domain of Prickle

cd09827
Location:82 → 178

PET_Prickle; The PET domain of Prickle
NM_001134459.2 → NP_001127931.1 prickle-like protein 2 isoform b

See identical proteins and their annotated locations for NP_001127931.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR and the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. Variants 2, 3 and 4 encode the same isoform (b), which has a shorter N-terminus compared to isoform a.

Source sequence(s)

AC122429, AC164636

Consensus CDS

CCDS51854.1

UniProtKB/Swiss-Prot

A6H652, Q80Y24

Related

ENSMUSP00000109073.2, ENSMUST00000113446.8

Conserved Domains (4) summary

cd09415
Location:130 → 188

LIM1_Prickle; The first LIM domain of Prickle

cd09418
Location:193 → 248

LIM2_Prickle; The second LIM domain of Prickle

cd09420
Location:253 → 311

LIM3_Prickle; The third LIM domain of Prickle

cd09827
Location:26 → 122

PET_Prickle; The PET domain of Prickle
NM_001134460.3 → NP_001127932.1 prickle-like protein 2 isoform b

See identical proteins and their annotated locations for NP_001127932.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) differs in the 5' UTR and the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. Variants 2, 3 and 4 encode the same isoform (b), which has a shorter N-terminus compared to isoform a.

Source sequence(s)

AC122429, AC164636

Consensus CDS

CCDS51854.1

UniProtKB/Swiss-Prot

A6H652, Q80Y24

Related

ENSMUSP00000109072.2, ENSMUST00000113445.2

Conserved Domains (4) summary

cd09415
Location:130 → 188

LIM1_Prickle; The first LIM domain of Prickle

cd09418
Location:193 → 248

LIM2_Prickle; The second LIM domain of Prickle

cd09420
Location:253 → 311

LIM3_Prickle; The third LIM domain of Prickle

cd09827
Location:26 → 122

PET_Prickle; The PET domain of Prickle
NM_001134461.2 → NP_001127933.1 prickle-like protein 2 isoform b

See identical proteins and their annotated locations for NP_001127933.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. Variants 2, 3 and 4 encode the same isoform (b), which has a shorter N-terminus compared to isoform a.

Source sequence(s)

AC122429, AC164636

Consensus CDS

CCDS51854.1

UniProtKB/Swiss-Prot

A6H652, Q80Y24

Related

ENSMUSP00000109074.2, ENSMUST00000113447.8

Conserved Domains (4) summary

cd09415
Location:130 → 188

LIM1_Prickle; The first LIM domain of Prickle

cd09418
Location:193 → 248

LIM2_Prickle; The second LIM domain of Prickle

cd09420
Location:253 → 311

LIM3_Prickle; The third LIM domain of Prickle

cd09827
Location:26 → 122

PET_Prickle; The PET domain of Prickle
NM_001404086.1 → NP_001391015.1 prickle-like protein 2 isoform c

Status: VALIDATED

Source sequence(s)

AC164636, AC166101

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000072.7 Reference GRCm39 C57BL/6J

Range

92347876..92683233 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036166119.1 → XP_036022012.1 prickle-like protein 2 isoform X3

UniProtKB/Swiss-Prot

A6H652, Q80Y24

Conserved Domains (4) summary

cd09415
Location:130 → 188

LIM1_Prickle; The first LIM domain of Prickle

cd09418
Location:193 → 248

LIM2_Prickle; The second LIM domain of Prickle

cd09420
Location:253 → 311

LIM3_Prickle; The third LIM domain of Prickle

cd09827
Location:26 → 122

PET_Prickle; The PET domain of Prickle
XM_030255385.2 → XP_030111245.1 prickle-like protein 2 isoform X2

Conserved Domains (4) summary

cd09415
Location:171 → 229

LIM1_Prickle; The first LIM domain of Prickle

cd09418
Location:234 → 289

LIM2_Prickle; The second LIM domain of Prickle

cd09420
Location:294 → 352

LIM3_Prickle; The third LIM domain of Prickle

cd09827
Location:67 → 163

PET_Prickle; The PET domain of Prickle