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Tlk2 tousled-like kinase 2 (Arabidopsis) [ Mus musculus (house mouse) ]

Gene ID: 24086, updated on 21-Apr-2024

Summary

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Official Symbol
Tlk2provided by MGI
Official Full Name
tousled-like kinase 2 (Arabidopsis)provided by MGI
Primary source
MGI:MGI:1346023
See related
Ensembl:ENSMUSG00000020694 AllianceGenome:MGI:1346023
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Tlk; PKUalpha; PKU-alpha; 4933403M19Rik
Summary
Predicted to enable ATP binding activity; identical protein binding activity; and protein serine/threonine kinase activity. Predicted to be involved in several processes, including cellular response to gamma radiation; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; and peptidyl-serine phosphorylation. Predicted to act upstream of or within several processes, including chromatin organization; phosphorylation; and spermatogenesis. Located in intermediate filament; nucleus; and perinuclear region of cytoplasm. Is expressed in several structures, including genitourinary system; heart; liver; lung; and spleen. Human ortholog(s) of this gene implicated in autosomal dominant non-syndromic intellectual disability. Orthologous to human TLK2 (tousled like kinase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in testis adult (RPKM 7.8), CNS E11.5 (RPKM 5.0) and 27 other tissues See more
Orthologs
human all
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Genomic context

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Location:
11 E1; 11 68.61 cM
Exon count:
24
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 11 NC_000077.7 (105066869..105174785)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 11 NC_000077.6 (105167895..105283959)

Chromosome 11 - NC_000077.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E7076 Neighboring gene methyltransferase 2, methylcytidine Neighboring gene RIKEN cDNA 1700052K11 gene Neighboring gene predicted gene 11620 Neighboring gene STARR-positive B cell enhancer ABC_E8463 Neighboring gene STARR-seq mESC enhancer starr_30905 Neighboring gene predicted gene, 53649 Neighboring gene mannose receptor, C type 2 Neighboring gene STARR-positive B cell enhancer mm9_chr11:105209236-105209536

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Tlk, a novel evolutionarily conserved murine serine threonine kinase, encodes multiple testis transcripts. Shalom S, et al. Mol Reprod Dev, 1999 Apr. PMID 10092119
  2. Discovery of Stromal Regulatory Networks that Suppress Ras-Sensitized Epithelial Cell Proliferation. Liu H, et al. Dev Cell, 2017 May 22. PMID 28535374, Free PMC Article
  3. Nuclear localization of protein kinase U-alpha is regulated by 14-3-3. Zhang S, et al. J Biol Chem, 1999 Aug 27. PMID 10455159
  4. The circadian E3 ligase complex SCF(FBXL3+CRY) targets TLK2. Correia SP, et al. Sci Rep, 2019 Jan 17. PMID 30655559, Free PMC Article
  5. Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication. Silljé HH, et al. EMBO J, 1999 Oct 15. PMID 10523312, Free PMC Article

See all (26) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. TLK1 and TLK2 have largely redundant roles in genome maintenance.
    Title: Differential requirements for Tousled-like kinases 1 and 2 in mammalian development.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Gene trapped (1) 
  • Targeted (6)  1 citation
  • Endonuclease-mediated (2) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding ISO
Inferred from Sequence Orthology
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein serine/threonine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein serine/threonine kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA damage response ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within cell cycle IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cell differentiation IEA
Inferred from Electronic Annotation
more info
  
involved_in cellular response to gamma radiation ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within chromatin organization IEA
Inferred from Electronic Annotation
more info
  
involved_in chromosome segregation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in chromosome segregation ISO
Inferred from Sequence Orthology
more info
  
involved_in intracellular signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in intracellular signal transduction ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within multicellular organism development IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of proteasomal ubiquitin-dependent protein catabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in nucleus localization ISO
Inferred from Sequence Orthology
more info
  
involved_in peptidyl-serine phosphorylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in peptidyl-serine phosphorylation ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within phosphorylation IEA
Inferred from Electronic Annotation
more info
  
involved_in protein phosphorylation ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of chromatin organization ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within spermatogenesis IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
  
located_in intermediate filament IDA
Inferred from Direct Assay
more info
 PubMed 
located_in intermediate filament ISO
Inferred from Sequence Orthology
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  
is_active_in perinuclear region of cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in perinuclear region of cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in perinuclear region of cytoplasm ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
serine/threonine-protein kinase tousled-like 2
Names
protein kinase U-alpha
NP_001106176.1
NP_001281258.1
NP_001281259.1
NP_001281260.1
NP_001281263.1
NP_001348925.1
XP_006533375.1
XP_006533376.1
XP_006533379.2
XP_006533383.1
XP_011247316.1
XP_030101858.1
XP_030101859.1
XP_030101861.1
XP_030101862.1
XP_036012594.1
XP_036012595.1
XP_036012596.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001112705.2 → NP_001106176.1  serine/threonine-protein kinase tousled-like 2 isoform A

    See identical proteins and their annotated locations for NP_001106176.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (A) encodes the longest isoform (A).
    Source sequence(s)
    AL645471
    Consensus CDS
    CCDS48954.1
    UniProtKB/TrEMBL
    B1ASU9
    Related
    ENSMUSP00000102554.3, ENSMUST00000106941.9
    Conserved Domains (2) summary
    COG1196
    Location:206 → 503
    Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
    cd14041
    Location:433 → 741
    STKc_TLK2; Catalytic domain of the Serine/Threonine kinase, Tousled-Like Kinase 2

  2. NM_001294329.1 → NP_001281258.1  serine/threonine-protein kinase tousled-like 2 isoform C

    See identical proteins and their annotated locations for NP_001281258.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (C) differs in the 5' UTR and lacks two alternate exons in the coding region compared to variant A; the 5'-most initiation codon, as used in variant A, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the alternate AUG to encode an isoform (C) that has a distinct N-terminus, and is shorter, compared to isoform A.
    Source sequence(s)
    AL645471
    Conserved Domains (2) summary
    COG1196
    Location:102 → 399
    Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
    cd14041
    Location:329 → 637
    STKc_TLK2; Catalytic domain of the Serine/Threonine kinase, Tousled-Like Kinase 2

  3. NM_001294330.1 → NP_001281259.1  serine/threonine-protein kinase tousled-like 2 isoform D

    Status: VALIDATED

    Description
    Transcript Variant: This variant (D) lacks two alternate in-frame exons in the 5' coding region and its 3' terminal exon extends past a splice site that is used in variant A. This results in a novel 3' coding region and 3' UTR compared to variant A. The encoded isoform (D) is shorter, and has a distinct C-terminus, compared to isoform A.
    Source sequence(s)
    AL645471, BC066198
    Consensus CDS
    CCDS79063.1
    UniProtKB/TrEMBL
    B7ZC85, Q6NZC4
    Related
    ENSMUSP00000118520.2, ENSMUST00000145048.8
    Conserved Domains (2) summary
    smart00220
    Location:387 → 592
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cl21453
    Location:369 → 593
    PKc_like; Protein Kinases, catalytic domain

  4. NM_001294331.1 → NP_001281260.1  serine/threonine-protein kinase tousled-like 2 isoform B

    See identical proteins and their annotated locations for NP_001281260.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (B) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant A. The encoded isoform (B) is shorter than isoform A.
    Source sequence(s)
    AL645471
    Consensus CDS
    CCDS36356.2
    UniProtKB/Swiss-Prot
    B1ASU7, B1ASU8, O55047, Q9D5Y5
    UniProtKB/TrEMBL
    Q6NZC4
    Related
    ENSMUSP00000102552.3, ENSMUST00000106939.9
    Conserved Domains (2) summary
    COG1196
    Location:174 → 471
    Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
    cd14041
    Location:401 → 709
    STKc_TLK2; Catalytic domain of the Serine/Threonine kinase, Tousled-Like Kinase 2

  5. NM_001294334.1 → NP_001281263.1  serine/threonine-protein kinase tousled-like 2 isoform E

    Status: VALIDATED

    Description
    Transcript Variant: This variant (E) contains a 3' terminal exon that extends past a splice site that is used in variant A. This results in a novel 3' coding region and 3' UTR compared to variant A. The encoded isoform (E) is shorter, and has a distinct C-terminus, compared to isoform A.
    Source sequence(s)
    AK014829, AL645471, BC066198
    Consensus CDS
    CCDS79062.1
    UniProtKB/TrEMBL
    Q6NZC4
    Related
    ENSMUSP00000090198.4, ENSMUST00000092537.10
    Conserved Domains (2) summary
    smart00220
    Location:451 → 656
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cl21453
    Location:433 → 657
    PKc_like; Protein Kinases, catalytic domain

  6. NM_001361996.1 → NP_001348925.1  serine/threonine-protein kinase tousled-like 2 isoform F

    Status: VALIDATED

    Source sequence(s)
    AL645471, AW490750
    UniProtKB/TrEMBL
    Q6NZC4
    Conserved Domains (1) summary
    cd14041
    Location:369 → 677
    STKc_TLK2; Catalytic domain of the Serine/Threonine kinase, Tousled-Like Kinase 2

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000077.7 Reference GRCm39 C57BL/6J

    Range
    105066869..105174785
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006533312.5 → XP_006533375.1  serine/threonine-protein kinase tousled-like 2 isoform X1

    See identical proteins and their annotated locations for XP_006533375.1

    UniProtKB/TrEMBL
    B1ASU9
    Conserved Domains (2) summary
    COG1196
    Location:206 → 503
    Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
    cd14041
    Location:433 → 741
    STKc_TLK2; Catalytic domain of the Serine/Threonine kinase, Tousled-Like Kinase 2

  2. XM_036156701.1 → XP_036012594.1  serine/threonine-protein kinase tousled-like 2 isoform X4

    UniProtKB/Swiss-Prot
    B1ASU7, B1ASU8, O55047, Q9D5Y5
    UniProtKB/TrEMBL
    Q6NZC4
    Conserved Domains (2) summary
    COG1196
    Location:174 → 471
    Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
    cd14041
    Location:401 → 709
    STKc_TLK2; Catalytic domain of the Serine/Threonine kinase, Tousled-Like Kinase 2

  3. XM_036156702.1 → XP_036012595.1  serine/threonine-protein kinase tousled-like 2 isoform X6

    UniProtKB/TrEMBL
    Q6NZC4
    Conserved Domains (1) summary
    cd14041
    Location:364 → 672
    STKc_TLK2; Catalytic domain of the Serine/Threonine kinase, Tousled-Like Kinase 2

  4. XM_030246002.2 → XP_030101862.1  serine/threonine-protein kinase tousled-like 2 isoform X7

    UniProtKB/TrEMBL
    Q6NZC4
    Conserved Domains (1) summary
    cd14041
    Location:338 → 646
    STKc_TLK2; Catalytic domain of the Serine/Threonine kinase, Tousled-Like Kinase 2

  5. XM_030246001.2 → XP_030101861.1  serine/threonine-protein kinase tousled-like 2 isoform X5

    UniProtKB/TrEMBL
    Q6NZC4
    Conserved Domains (1) summary
    cd14041
    Location:370 → 678
    STKc_TLK2; Catalytic domain of the Serine/Threonine kinase, Tousled-Like Kinase 2

  6. XM_011249014.4 → XP_011247316.1  serine/threonine-protein kinase tousled-like 2 isoform X4

    See identical proteins and their annotated locations for XP_011247316.1

    UniProtKB/Swiss-Prot
    B1ASU7, B1ASU8, O55047, Q9D5Y5
    UniProtKB/TrEMBL
    Q6NZC4
    Related
    ENSMUSP00000015107.7, ENSMUST00000015107.13
    Conserved Domains (2) summary
    COG1196
    Location:174 → 471
    Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
    cd14041
    Location:401 → 709
    STKc_TLK2; Catalytic domain of the Serine/Threonine kinase, Tousled-Like Kinase 2

  7. XM_030245999.2 → XP_030101859.1  serine/threonine-protein kinase tousled-like 2 isoform X3

    UniProtKB/TrEMBL
    Q6NZC4
    Conserved Domains (2) summary
    COG1196
    Location:182 → 471
    Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
    cd14041
    Location:401 → 709
    STKc_TLK2; Catalytic domain of the Serine/Threonine kinase, Tousled-Like Kinase 2

  8. XM_030245998.2 → XP_030101858.1  serine/threonine-protein kinase tousled-like 2 isoform X1

    UniProtKB/TrEMBL
    B1ASU9
    Conserved Domains (2) summary
    COG1196
    Location:206 → 503
    Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
    cd14041
    Location:433 → 741
    STKc_TLK2; Catalytic domain of the Serine/Threonine kinase, Tousled-Like Kinase 2

  9. XM_006533313.5 → XP_006533376.1  serine/threonine-protein kinase tousled-like 2 isoform X1

    See identical proteins and their annotated locations for XP_006533376.1

    UniProtKB/TrEMBL
    B1ASU9
    Conserved Domains (2) summary
    COG1196
    Location:206 → 503
    Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
    cd14041
    Location:433 → 741
    STKc_TLK2; Catalytic domain of the Serine/Threonine kinase, Tousled-Like Kinase 2

  10. XM_036156703.1 → XP_036012596.1  serine/threonine-protein kinase tousled-like 2 isoform X4

    UniProtKB/Swiss-Prot
    B1ASU7, B1ASU8, O55047, Q9D5Y5
    UniProtKB/TrEMBL
    Q6NZC4
    Conserved Domains (2) summary
    COG1196
    Location:174 → 471
    Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
    cd14041
    Location:401 → 709
    STKc_TLK2; Catalytic domain of the Serine/Threonine kinase, Tousled-Like Kinase 2

  11. XM_006533320.5 → XP_006533383.1  serine/threonine-protein kinase tousled-like 2 isoform X8

    See identical proteins and their annotated locations for XP_006533383.1

    Conserved Domains (2) summary
    COG1196
    Location:102 → 399
    Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
    cd14041
    Location:329 → 637
    STKc_TLK2; Catalytic domain of the Serine/Threonine kinase, Tousled-Like Kinase 2

  12. XM_006533316.5 → XP_006533379.2  serine/threonine-protein kinase tousled-like 2 isoform X2

    Conserved Domains (1) summary
    cd14041
    Location:396 → 704
    STKc_TLK2; Catalytic domain of the Serine/Threonine kinase, Tousled-Like Kinase 2

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_011903.3: Suppressed sequence

    Description
    NM_011903.3: This RefSeq was permanently suppressed because currently there is not sufficient data to support this transcript.
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O55047.2 GenPept UniProtKB/Swiss-Prot:O55047

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