Ttll8 tubulin tyrosine ligase-like family, member 8 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ttll8provided by MGI
Official Full Name: tubulin tyrosine ligase-like family, member 8provided by MGI
Primary source: MGI:MGI:1922902
See related: Ensembl:ENSMUSG00000022388 AllianceGenome:MGI:1922902
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 4933401B17; 1700019P01Rik
Summary: Enables protein-glycine ligase activity, initiating. Involved in protein polyglycylation. Acts upstream of or within cilium assembly. Located in axoneme and microtubule cytoskeleton. Orthologous to human TTLL8 (tubulin tyrosine ligase like 8). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Restricted expression toward testis adult (RPKM 37.7) See more
Orthologs: human all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 15 E3; 15 44.39 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (88773981..88839258, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (88889778..88955051, complement)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help

See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility.
Title: Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility.
The TTLL8 was required for stability and maintenance of ependymal cilia, whereas the polyglutamylase TTLL6 was necessary for coordinated beating behavior.
Title: Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia.
TTLL10 can strongly glycylate tubulin in a TTLL8-dependent manner. Furthermore, these two TTLL proteins can glycylate unidentified 170-, 110-, 75-, 40-, 35-, and 30-kDa acidic proteins.
Title: TTLL10 can perform tubulin glycylation when co-expressed with TTLL8.

Submit: New GeneRIF Correction

Variation

Go to the top of the page Help

Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (5) 1 citation
Endonuclease-mediated (1)

General gene information

Go to the top of the page Help

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ligase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein-glycine ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein-glycine ligase activity, initiating	IBA Inferred from Biological aspect of Ancestor more info
enables protein-glycine ligase activity, initiating	IDA Inferred from Direct Assay more info	PubMed
enables protein-glycine ligase activity, initiating	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in cilium assembly	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cilium assembly	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cilium assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cilium movement	IBA Inferred from Biological aspect of Ancestor more info
involved_in flagellated sperm motility	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein modification process	IEA Inferred from Electronic Annotation more info
involved_in protein polyglycylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein polyglycylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein polyglycylation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in axoneme	IBA Inferred from Biological aspect of Ancestor more info
located_in axoneme	IDA Inferred from Direct Assay more info	PubMed
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cilium	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
is_active_in microtubule cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in microtubule cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in motile cilium	IEA Inferred from Electronic Annotation more info
located_in sperm flagellum	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: protein monoglycylase TTLL8

Names: TTL domain protein; tubulin--tyrosine ligase-like protein 8

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001357736.2 → NP_001344665.1 protein monoglycylase TTLL8 isoform 2

Status: VALIDATED

Source sequence(s)

AC119959

Conserved Domains (1) summary

pfam03133
Location:193 → 415

TTL; Tubulin-tyrosine ligase family
NM_001428803.1 → NP_001415732.1 protein monoglycylase TTLL8 isoform 3

Status: VALIDATED

Source sequence(s)

AC117700, AC119959
NM_001428811.1 → NP_001415740.1 protein monoglycylase TTLL8 isoform 3

Status: VALIDATED

Source sequence(s)

AC117700, AC119959
NM_172818.4 → NP_766406.3 protein monoglycylase TTLL8 isoform 1

Status: VALIDATED

Source sequence(s)

AC119959

Consensus CDS

CCDS27735.2

Related

ENSMUSP00000104996.2, ENSMUST00000109371.8

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000081.7 Reference GRCm39 C57BL/6J

Range

88773981..88839258 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036159403.1 → XP_036015296.1 protein monoglycylase TTLL8 isoform X3
XM_011245621.3 → XP_011243923.1 protein monoglycylase TTLL8 isoform X2

Conserved Domains (1) summary

pfam03133
Location:487 → 709

TTL; Tubulin-tyrosine ligase family
XM_006520959.5 → XP_006521022.1 protein monoglycylase TTLL8 isoform X4

See identical proteins and their annotated locations for XP_006521022.1

UniProtKB/TrEMBL

Q8C0V2

Conserved Domains (1) summary

pfam03133
Location:193 → 415

TTL; Tubulin-tyrosine ligase family
XM_006520955.4 → XP_006521018.1 protein monoglycylase TTLL8 isoform X1

Conserved Domains (1) summary

pfam03133
Location:487 → 709

TTL; Tubulin-tyrosine ligase family
XM_011245623.4 → XP_011243925.1 protein monoglycylase TTLL8 isoform X6
XM_011245622.4 → XP_011243924.1 protein monoglycylase TTLL8 isoform X5