MKRN2 makorin ring finger protein 2 [Homo sapiens (human)] - Gene

Summary

Official Symbol: MKRN2provided by HGNC
Official Full Name: makorin ring finger protein 2provided by HGNC
Primary source: HGNC:HGNC:7113
See related: Ensembl:ENSG00000075975 MIM:608426; AllianceGenome:HGNC:7113
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: RNF62; HSPC070
Summary: This gene encodes a probable E3 ubiquitin ligase containing several zinc finger domains, that is a member of the makorin RING zinc-finger protein family. This gene overlaps the v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1) gene in an antisense orientation and may have a co-regulatory function with RAF1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
Expression: Ubiquitous expression in testis (RPKM 30.6), thyroid (RPKM 16.5) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 3p25.2

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	3	NC_000003.12 (12557087..12583713)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	3	NC_060927.1 (12554891..12582517)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	3	NC_000003.11 (12598586..12625212)

Chromosome 3 - NC_000003.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

[Overexpression of MKRN2 Inhibits the Growth of Ovarian Cancer Cells].
Title: [Overexpression of MKRN2 Inhibits the Growth of Ovarian Cancer Cells].
MKRN1/2 serve as tumor suppressors in renal clear cell carcinoma by regulating the expression of p53.
Title: MKRN1/2 serve as tumor suppressors in renal clear cell carcinoma by regulating the expression of p53.
Integrated analysis of exosomal lncRNA and mRNA expression profiles reveals the involvement of lnc-MKRN2-42:1 in the pathogenesis of Parkinson's disease.
Title: Integrated analysis of exosomal lncRNA and mRNA expression profiles reveals the involvement of lnc-MKRN2-42:1 in the pathogenesis of Parkinson's disease.
Ubiquitination of IGF2BP3 by E3 ligase MKRN2 regulates the proliferation and migration of human neuroblastoma SHSY5Y cells.
Title: Ubiquitination of IGF2BP3 by E3 ligase MKRN2 regulates the proliferation and migration of human neuroblastoma SHSY5Y cells.
Results suggested that MKRN2 inhibited NSCLC progression by reducing the metastatic potential of cancer cells. Our findings provide critical insight into the association of MKRN2 expression with favorable clinicopathological characteristics in NSCLC patients.
Title: MKRN2 inhibits migration and invasion of non-small-cell lung cancer by negatively regulating the PI3K/Akt pathway.
These results delineate a novel role of MKRN2 in negatively regulating NF-kappaB-mediated inflammatory responses, cooperatively with PDLIM2.
Title: MKRN2 is a novel ubiquitin E3 ligase for the p65 subunit of NF-κB and negatively regulates inflammatory responses.
Over-expression of MKRN2 in K562 cells increased cell proliferation. Our results indicated possible roles of MKRN2 in normal and malignant hematopoiesis.
Title: Ubiquitous expression of MAKORIN-2 in normal and malignant hematopoietic cells and its growth promoting activity.
Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Single nucleotide polymorphisms of matrix metalloproteinase 9 (MMP9) and tumor protein 73 (TP73) interact with Epstein-Barr virus in chronic lymphocytic leukemia: results from the European case-control study EpiLymph.
MKRN2 and RAF1 are co-expressed in tissues and cell lines
Title: Phylogenetic conservation of the makorin-2 gene, encoding a multiple zinc-finger protein, antisense to the RAF1 proto-oncogene.

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Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Replication interactions

Interaction	Pubs
Knockdown of makorin ring finger protein 2 (MKRN2) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

G32929 (e-PCR)
- UniSTS:117507

D3S4000 (e-PCR)
- UniSTS:8196

G15882 (e-PCR)
- UniSTS:44344

GDB:217154 (e-PCR)
- UniSTS:156230

RH123901 (e-PCR)
- UniSTS:136276

D3S3701 (e-PCR), detects polymorphism
- UniSTS:31609

A009W45 (e-PCR)
- UniSTS:64891

D3S2870E (e-PCR)
- UniSTS:81285

SHGC-112307 (e-PCR)
- UniSTS:168607

SHGC-76728 (e-PCR)
- UniSTS:77485

RH102412 (e-PCR)
- UniSTS:96746

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables RNA binding	HDA more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in DNA-templated transcription	ISS Inferred from Sequence or Structural Similarity more info
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of inflammatory response to antigenic stimulus	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of non-canonical NF-kappaB signal transduction	ISS Inferred from Sequence or Structural Similarity more info
involved_in phosphatidylinositol 3-kinase/protein kinase B signal transduction	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein polyubiquitination	IEA Inferred from Electronic Annotation more info
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in spermatogenesis	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin-dependent protein catabolic process	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase makorin-2

Names: RING finger protein 62; RING-type E3 ubiquitin transferase makorin-2; makorin RING zinc-finger protein 2; probable E3 ubiquitin-protein ligase makorin-2

NP_001258636.1

EC 2.3.2.27

NP_054879.3

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001271707.2 → NP_001258636.1 E3 ubiquitin-protein ligase makorin-2 isoform 2

See identical proteins and their annotated locations for NP_001258636.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an internal exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.

Source sequence(s)

AK298463, BC015715

Consensus CDS

CCDS63545.1

UniProtKB/TrEMBL

C9J494

Related

ENSP00000396340.1, ENST00000411987.5

Conserved Domains (2) summary

PHA03096
Location:135 → 284

PHA03096; p28-like protein; Provisional

cd00162
Location:195 → 249

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
NM_014160.5 → NP_054879.3 E3 ubiquitin-protein ligase makorin-2 isoform 1

See identical proteins and their annotated locations for NP_054879.3

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AK298463, BC015715

Consensus CDS

CCDS33702.1

UniProtKB/Swiss-Prot

A6NIA2, B3KRC5, B4DPR4, Q8N391, Q96BD4, Q9BUY2, Q9H000, Q9NRY1

UniProtKB/TrEMBL

A0A7I2YQI0

Related

ENSP00000170447.7, ENST00000170447.12

Conserved Domains (3) summary

smart00356
Location:35 → 55

ZnF_C3H1; zinc finger

PHA03096
Location:178 → 327

PHA03096; p28-like protein; Provisional

cd00162
Location:238 → 292

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...