Scap SREBF chaperone [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Scapprovided by MGI
Official Full Name: SREBF chaperoneprovided by MGI
Primary source: MGI:MGI:2135958
See related: Ensembl:ENSMUSG00000032485 AllianceGenome:MGI:2135958
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: mKIAA0199; 9530044G19
Summary: Predicted to enable cholesterol binding activity. Acts upstream of or within several processes, including cholesterol metabolic process; regulation of lipid biosynthetic process; and response to insulin. Located in endoplasmic reticulum membrane. Is expressed in 1st branchial arch maxillary component; brain; heart; liver; and sensory organ. Orthologous to human SCAP (SREBF chaperone). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in ovary adult (RPKM 50.5), adrenal adult (RPKM 43.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 F2; 9 59.91 cM

Exon count:: 25

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (110162356..110214017)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (110333288..110384949)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The role of KLF2 in regulating hepatic lipogenesis and blood cholesterol homeostasis via the SCAP/SREBP pathway.
Title: The role of KLF2 in regulating hepatic lipogenesis and blood cholesterol homeostasis via the SCAP/SREBP pathway.
SCAP contributes to embryonic angiogenesis by negatively regulating KISS-1 expression in mice.
Title: SCAP contributes to embryonic angiogenesis by negatively regulating KISS-1 expression in mice.
SCAP deficiency facilitates obesity and insulin resistance through shifting adipose tissue macrophage polarization.
Title: SCAP deficiency facilitates obesity and insulin resistance through shifting adipose tissue macrophage polarization.
Macrophage SCAP Contributes to Metaflammation and Lean NAFLD by Activating STING-NF-kappaB Signaling Pathway.
Title: Macrophage SCAP Contributes to Metaflammation and Lean NAFLD by Activating STING-NF-κB Signaling Pathway.
Inhibiting SCAP/SREBP exacerbates liver injury and carcinogenesis in murine nonalcoholic steatohepatitis.
Title: Inhibiting SCAP/SREBP exacerbates liver injury and carcinogenesis in murine nonalcoholic steatohepatitis.
DHT causes liver steatosis via transcriptional regulation of SCAP in normal weight female mice.
Title: DHT causes liver steatosis via transcriptional regulation of SCAP in normal weight female mice.
Autophagy in VSMCs was increased in SM22alpha-Cre:SCAP(flox/+):ApoE(-/-) mice.
Title: SCAP knockdown in vascular smooth muscle cells alleviates atherosclerosis plaque formation via up-regulating autophagy in ApoE(-/-) mice.
Intracellular biosynthesis of lipids and cholesterol by Scap and Insig in mesenchymal cells regulates long bone growth and chondrocyte homeostasis.
Title: Intracellular biosynthesis of lipids and cholesterol by Scap and Insig in mesenchymal cells regulates long bone growth and chondrocyte homeostasis.
Mechanistic studies show that RNF145 triggers ubiquitination of SCAP on lysine residues within a cytoplasmic loop essential for COPII binding, potentially inhibiting its transport to Golgi and subsequent processing of SREBP-2.
Title: Inhibition of cholesterol biosynthesis through RNF145-dependent ubiquitination of SCAP.
Reexpression of SCAP in SCAP-deficient cells restored SREBP2 protein expression and partially restored steroidogenic responses, confirming the requirement of SCAP-SREBP2 in steroidogenesis.
Title: SCAP/SREBP pathway is required for the full steroidogenic response to cyclic AMP.

Submit: New GeneRIF Correction See all GeneRIFs (24)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Transgenic (1)
Gene trapped (1)
Targeted (3) 1 citation
Endonuclease-mediated (6)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

BB347385 (e-PCR)
- UniSTS:208458

AI462446 (e-PCR)
- UniSTS:165816

UniSTS:236259 (e-PCR)
- UniSTS:236259

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables cholesterol binding	TAS Traceable Author Statement more info	PubMed
enables lipid binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables sterol binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in SREBP signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular lipid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cholesterol metabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within immune response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cholesterol biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of cholesterol biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of cholesterol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of fatty acid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of fatty acid metabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to hypoxia	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to insulin	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within steroid metabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi membrane	ISO Inferred from Sequence Orthology more info
part_of SREBP-SCAP complex	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
is_active_in endoplasmic reticulum membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: sterol regulatory element-binding protein cleavage-activating protein

Names: SREBP cleavage activating protein; Sreb cleavage-activating protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001001144.3 → NP_001001144.2 sterol regulatory element-binding protein cleavage-activating protein

See identical proteins and their annotated locations for NP_001001144.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.

Source sequence(s)

BC070437, BC072633, BM250223

Consensus CDS

CCDS23564.1

UniProtKB/Swiss-Prot

Q6A0A6, Q6GQT6, Q6NS67, Q7TNG7, Q80UI6

Related

ENSMUSP00000095953.4, ENSMUST00000098350.10

Conserved Domains (5) summary

smart00320
Location:771 → 802

WD40; WD40 repeats

TIGR00917
Location:11 → 466

2A060601; Niemann-Pick C type protein family

sd00039
Location:957 → 998

7WD40; WD40 repeat [structural motif]

pfam12349
Location:308 → 454

Sterol-sensing; Sterol-sensing domain of SREBP cleavage-activation

cl29593
Location:1065 → 1232

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
NM_001103162.2 → NP_001096632.1 sterol regulatory element-binding protein cleavage-activating protein

See identical proteins and their annotated locations for NP_001096632.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.

Source sequence(s)

AK150275, BC070437, BM250223, BY311416

Consensus CDS

CCDS23564.1

UniProtKB/Swiss-Prot

Q6A0A6, Q6GQT6, Q6NS67, Q7TNG7, Q80UI6

Conserved Domains (5) summary

smart00320
Location:771 → 802

WD40; WD40 repeats

TIGR00917
Location:11 → 466

2A060601; Niemann-Pick C type protein family

sd00039
Location:957 → 998

7WD40; WD40 repeat [structural motif]

pfam12349
Location:308 → 454

Sterol-sensing; Sterol-sensing domain of SREBP cleavage-activation

cl29593
Location:1065 → 1232

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
NM_001410582.1 → NP_001397511.1 sterol regulatory element-binding protein cleavage-activating protein

Status: VALIDATED

Source sequence(s)

AC160104

UniProtKB/Swiss-Prot

Q6A0A6, Q6GQT6, Q6NS67, Q7TNG7, Q80UI6

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

110162356..110214017

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006512084.4 → XP_006512147.1 sterol regulatory element-binding protein cleavage-activating protein isoform X1

See identical proteins and their annotated locations for XP_006512147.1

UniProtKB/Swiss-Prot

Q6A0A6, Q6GQT6, Q6NS67, Q7TNG7, Q80UI6

Conserved Domains (5) summary

smart00320
Location:771 → 802

WD40; WD40 repeats

TIGR00917
Location:11 → 466

2A060601; Niemann-Pick C type protein family

sd00039
Location:957 → 998

7WD40; WD40 repeat [structural motif]

pfam12349
Location:308 → 454

Sterol-sensing; Sterol-sensing domain of SREBP cleavage-activation

cl29593
Location:1065 → 1232

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
XM_006512085.4 → XP_006512148.1 sterol regulatory element-binding protein cleavage-activating protein isoform X1

See identical proteins and their annotated locations for XP_006512148.1

UniProtKB/Swiss-Prot

Q6A0A6, Q6GQT6, Q6NS67, Q7TNG7, Q80UI6

Conserved Domains (5) summary

smart00320
Location:771 → 802

WD40; WD40 repeats

TIGR00917
Location:11 → 466

2A060601; Niemann-Pick C type protein family

sd00039
Location:957 → 998

7WD40; WD40 repeat [structural motif]

pfam12349
Location:308 → 454

Sterol-sensing; Sterol-sensing domain of SREBP cleavage-activation

cl29593
Location:1065 → 1232

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...