Insig1 insulin induced gene 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Insig1provided by MGI
Official Full Name: insulin induced gene 1provided by MGI
Primary source: MGI:MGI:1916289
See related: Ensembl:ENSMUSG00000045294 AllianceGenome:MGI:1916289
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Insig-1; 1810013C12Rik
Summary: Predicted to enable oxysterol binding activity. Acts upstream of or within several processes, including ear morphogenesis; negative regulation of fat cell differentiation; and negative regulation of lipid biosynthetic process. Predicted to be located in membrane. Predicted to be integral component of membrane. Predicted to be part of SREBP-SCAP-Insig complex. Predicted to be active in endoplasmic reticulum. Is expressed in several structures, including alimentary system; ganglia; neural retina; telencephalon ventricular layer; and vibrissa. Orthologous to human INSIG1 (insulin induced gene 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in liver adult (RPKM 186.8), adrenal adult (RPKM 174.3) and 26 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 B1; 5 13.94 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (28276410..28283660)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (28071412..28078662)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Suppression of insulin-induced gene 1 (INSIG1) function promotes hepatic lipid remodelling and restrains NASH progression.
Title: Suppression of insulin-induced gene 1 (INSIG1) function promotes hepatic lipid remodelling and restrains NASH progression.
Intracellular biosynthesis of lipids and cholesterol by Scap and Insig in mesenchymal cells regulates long bone growth and chondrocyte homeostasis.
Title: Intracellular biosynthesis of lipids and cholesterol by Scap and Insig in mesenchymal cells regulates long bone growth and chondrocyte homeostasis.
myeloid-cell-specific Insig1-/- mice are more susceptible to HSV-1 infection
Title: The E3 ubiquitin ligase AMFR and INSIG1 bridge the activation of TBK1 kinase by modifying the adaptor STING.
the essential role of Insig1,2 proteins in the sterol homeostasis of enterocytes.
Title: Insig proteins mediate feedback inhibition of cholesterol synthesis in the intestine.
reducing Insig1 mRNA to a similar degree observed in morbidly obese human WAT accelerates adipocyte differentiation and enhances lipogenesis, and consequently, lipid accumulation
Title: Adaptive changes of the Insig1/SREBP1/SCD1 set point help adipose tissue to cope with increased storage demands of obesity.
we consistently observe involvement of gp78 in Insig-1 degradation, we find no substantive evidence to support roles for either gp78 or TRC8 in the robust sterol-accelerated degradation of HMG-CoA reductase.
Title: Differential regulation of HMG-CoA reductase and Insig-1 by enzymes of the ubiquitin-proteasome system.
analysis of mRNA abundance and expression of SLC27A, ACC, SCD, FADS, LPIN, INSIG, and PPARGC1 gene isoforms in mouse mammary glands during the lactation cycle
Title: mRNA abundance and expression of SLC27A, ACC, SCD, FADS, LPIN, INSIG, and PPARGC1 gene isoforms in mouse mammary glands during the lactation cycle.
Insig deficiency in skin causes accumulation of cholesterol precursors, and this impairs normal hair development
Title: Hair growth defects in Insig-deficient mice caused by cholesterol precursor accumulation and reversed by simvastatin.
Silibinin inhibits adipocyte differentiation through a potential up-regulation of insig-1 and insig-2 at an early phase in adipocyte differentiation.
Title: Silibinin attenuates adipogenesis in 3T3-L1 preadipocytes through a potential upregulation of the insig pathway.
Insig-1 is a direct PPARdelta target gene in hepatocytes. PPARdelta inhibited SREBP-1 activation via induction of Insig-1. Overexpression of PPARdelta induced the expression of Insig-1, suppressed SREBP-1 activation, and ameliorated hepatic steatosis.
Title: Peroxisome proliferator-activated receptor-delta induces insulin-induced gene-1 and suppresses hepatic lipogenesis in obese diabetic mice.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (5)

General gene information

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Markers

Insig1 (e-PCR), detects polymorphism
- UniSTS:532555

PMC303355P1 (e-PCR)
- UniSTS:272504

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables lipid binding	IEA Inferred from Electronic Annotation more info
enables oxysterol binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein sequestering activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in SREBP signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in SREBP signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in SREBP-SCAP complex retention in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
involved_in SREBP-SCAP complex retention in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
involved_in cellular response to insulin stimulus	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to sterol	ISO Inferred from Sequence Orthology more info
involved_in cholesterol biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cholesterol biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cholesterol biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in cholesterol homeostasis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cholesterol metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cranial suture morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within inner ear morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within middle ear morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of cargo loading into COPII-coated vesicle	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of fat cell differentiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of fatty acid biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of protein exit from endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of steroid biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within response to sterol depletion	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within roof of mouth development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within steroid metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within sterol biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within triglyceride metabolic process	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of SREBP-SCAP-Insig complex	IBA Inferred from Biological aspect of Ancestor more info
part_of SREBP-SCAP-Insig complex	ISO Inferred from Sequence Orthology more info
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	TAS Traceable Author Statement more info	PubMed
is_active_in endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: insulin-induced gene 1 protein

Names: INSIG-1 membrane protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.