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Mettl8 methyltransferase 8, methylcytidine [ Mus musculus (house mouse) ]

Gene ID: 228019, updated on 21-Apr-2024

Summary

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Official Symbol
Mettl8provided by MGI
Official Full Name
methyltransferase 8, methylcytidineprovided by MGI
Primary source
MGI:MGI:2385142
See related
Ensembl:ENSMUSG00000041975 AllianceGenome:MGI:2385142
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Tip
Summary
This locus encodes a member of the methyltransferase family, and is involved in chromatin remodeling. Transcripts from this locus can be induced or inhibited by cell stretch and affect cell differentiation in the myogenic or adipogenic pathways. Multiple transcript variants encoding different isoforms have been found for this gene. Additional splice variants have been described in the literature but they meet nonsense-mediated decay (NMD) criteria and are likely to be degraded as soon as they are transcribed. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in thymus adult (RPKM 2.3), CNS E11.5 (RPKM 2.1) and 28 other tissues See more
Orthologs
human all
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Genomic context

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Location:
2 C2; 2 42.34 cM
Exon count:
11
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (70794902..70886729, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (70964561..71056388, complement)

Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene predicted gene, 54159 Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:70768788-70768989 Neighboring gene STARR-positive B cell enhancer ABC_E5929 Neighboring gene STARR-positive B cell enhancer ABC_E5930 Neighboring gene predicted gene, 39843 Neighboring gene STARR-positive B cell enhancer ABC_E11137 Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:70885116-70885299 Neighboring gene STARR-positive B cell enhancer ABC_E509 Neighboring gene DDB1 and CUL4 associated factor 17 Neighboring gene STARR-positive B cell enhancer ABC_E4465 Neighboring gene cytochrome b reductase 1 Neighboring gene predicted gene, 39576

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. The STAT3 Target Mettl8 Regulates Mouse ESC Differentiation via Inhibiting the JNK Pathway. Gu H, et al. Stem Cell Reports, 2018 Jun 5. PMID 29706498, Free PMC Article
  2. MiR-208b Regulates the Conversion of Skeletal Muscle Fiber Types by Inhibiting Mettl8 Expression. Li X, et al. Front Genet, 2022. PMID 35281804, Free PMC Article
  3. Effects of the SANT domain of tension-induced/inhibited proteins (TIPs), novel partners of the histone acetyltransferase p300, on p300 activity and TIP-6-induced adipogenesis. Badri KR, et al. Mol Cell Biol, 2008 Oct. PMID 18710950, Free PMC Article
  4. TIPs are tension-responsive proteins involved in myogenic versus adipogenic differentiation. Jakkaraju S, et al. Dev Cell, 2005 Jul. PMID 15992539
  5. Epitranscriptomic regulation of cortical neurogenesis via Mettl8-dependent mitochondrial tRNA m(3)C modification. Zhang F, et al. Cell Stem Cell, 2023 Mar 2. PMID 36764294, Free PMC Article

See all (20) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. METTL8 prohibits the activation of JNK signaling and enhances the differentiation of mouse ESCs. Collectively, our study uncovers a STAT3 target, Mettl8, which regulates mouse ESC differentiation via JNK signaling.
    Title: The STAT3 Target Mettl8 Regulates Mouse ESC Differentiation via Inhibiting the JNK Pathway.
  2. TIP-6 was identified as a new member of the adipogenic cascade.
    Title: Effects of the SANT domain of tension-induced/inhibited proteins (TIPs), novel partners of the histone acetyltransferase p300, on p300 activity and TIP-6-induced adipogenesis.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC7995, MGC67805

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables histone acetyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables mRNA methyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables tRNA (cytidine-3-)-methyltransferase activity ISO
Inferred from Sequence Orthology
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within fat cell differentiation IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in mitochondrial tRNA modification ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of mitochondrial translation ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within skeletal muscle tissue development IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in tRNA C3-cytosine methylation ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within tRNA processing IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in mitochondrion ISO
Inferred from Sequence Orthology
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
tRNA N(3)-methylcytidine methyltransferase METTL8, mitochondrial
Names
mRNA N(3)-methylcytidine methyltransferase METTL8
methyltransferase like 8
methyltransferase-like protein 8
tension induced/inhibited protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001110512.3 → NP_001103982.1  tRNA N(3)-methylcytidine methyltransferase METTL8, mitochondrial isoform b

    See identical proteins and their annotated locations for NP_001103982.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (b), also known as TIP-5, uses an alternate in-frame splice site in the central coding region, compared to variant a. The resulting protein (isoform b), is shorter than isoform a.
    Source sequence(s)
    AL929228
    UniProtKB/Swiss-Prot
    A2AUU0
    Conserved Domains (1) summary
    pfam13649
    Location:114 → 217
    Methyltransf_25; Methyltransferase domain

  2. NM_145524.4 → NP_663499.2  tRNA N(3)-methylcytidine methyltransferase METTL8, mitochondrial isoform a

    Status: VALIDATED

    Description
    Transcript Variant: This variant (a), also known as TIP-4, represents the longer transcript and encodes the longer isoform (a).
    Source sequence(s)
    AL929228
    Consensus CDS
    CCDS50600.1
    UniProtKB/TrEMBL
    B0R0U1
    Related
    ENSMUSP00000107804.3, ENSMUST00000112186.9
    Conserved Domains (1) summary
    pfam13649
    Location:190 → 293
    Methyltransf_25; Methyltransferase domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000068.8 Reference GRCm39 C57BL/6J

    Range
    70794902..70886729 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036161117.1 → XP_036017010.1  tRNA N(3)-methylcytidine methyltransferase METTL8, mitochondrial isoform X1

    UniProtKB/TrEMBL
    B0R0U1
    Conserved Domains (1) summary
    cl17173
    Location:242 → 308
    AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...

  2. XM_006499196.4 → XP_006499259.1  tRNA N(3)-methylcytidine methyltransferase METTL8, mitochondrial isoform X2

    UniProtKB/TrEMBL
    Q8C994

RNA

  1. XR_004939846.1 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
A2AUU0.2 GenPept UniProtKB/Swiss-Prot:A2AUU0

Gene LinkOut

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