Cerk ceramide kinase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cerkprovided by MGI
Official Full Name: ceramide kinaseprovided by MGI
Primary source: MGI:MGI:2386052
See related: Ensembl:ENSMUSG00000035891 AllianceGenome:MGI:2386052
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: mKIAA1646; D330016D08Rik
Summary: Enables ceramide kinase activity. Predicted to be involved in ceramide metabolic process and phosphorylation. Located in mitochondrion. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; integumental system; and sensory organ. Orthologous to human CERK (ceramide kinase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in thymus adult (RPKM 55.0), cerebellum adult (RPKM 36.2) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 15 E2; 15 40.52 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (86023302..86070537, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (86139101..86186259, complement)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Effects of ceramide kinase knockout on lipopolysaccharide-treated sepsis-model mice: Changes in serum cytokine/chemokine levels and increased lethality.
Title: Effects of ceramide kinase knockout on lipopolysaccharide-treated sepsis-model mice: Changes in serum cytokine/chemokine levels and increased lethality.
Ceramide kinase knockout ameliorates multiple sclerosis-like behaviors and demyelination in cuprizone-treated mice.
Title: Ceramide kinase knockout ameliorates multiple sclerosis-like behaviors and demyelination in cuprizone-treated mice.
MicroRNA-34a causes ceramide accumulation and effects insulin signaling pathway by targeting ceramide kinase (CERK) in aging skeletal muscle.
Title: MicroRNA-34a causes ceramide accumulation and effects insulin signaling pathway by targeting ceramide kinase (CERK) in aging skeletal muscle.
the data presented in this work suggest that exogenous C1P may balance adipogenesis, and that targeting CerK may be a novel way for potential applications in the treatment of obesity or other inflammation-associated diseases
Title: Regulation of adipogenesis by ceramide 1-phosphate.
results suggest that intracellular ceramide kinase is required for the migration of bone marrow-derived mesenchymal stem cells, and the roles of the intrinsic ceramide kinase in the migration are associated with N-cadherin regulation
Title: Ceramide kinase regulates the migration of bone marrow-derived mesenchymal stem cells.
The depletion of CerK enhances the pathology of colitis and lethal responses in DSS-treated mice.
Title: Knockout of Ceramide Kinase Aggravates Pathological and Lethal Responses in Mice with Experimental Colitis.
these studies demonstrate a novel requirement for the CERK-derived C1P in the proper healing response of wounds.
Title: Ceramide kinase is required for a normal eicosanoid response and the subsequent orderly migration of fibroblasts.
there are significant differences in eicosanoid levels in ex vivo CERK(-/-) cells compared with wild-type counterparts, but the effect of the genetic ablation of CERK on eicosanoid synthesis and the serum levels of C1P was not apparent in vivo
Title: Characterization of eicosanoid synthesis in a genetic ablation model of ceramide kinase.
role of ceramide kinase in obesity and insulin resistance
Title: Ceramide kinase deficiency improves diet-induced obesity and insulin resistance.
Results indicate that C1P produced by CERK has a negative effect on the processing/secretion of TNFalpha via modulation of TACE activity.
Title: Ceramide kinase regulates the production of tumor necrosis factor α (TNFα) via inhibition of TNFα-converting enzyme.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Endonuclease-mediated (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

H05L03.2 (e-PCR)
- UniSTS:311318

AI848610 (e-PCR)
- UniSTS:180812

NoName (e-PCR)
- UniSTS:224931

Cerk (e-PCR), detects polymorphism
- UniSTS:506795

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ceramide kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ceramide kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables ceramide kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ceramide kinase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables dihydroceramide kinase activity	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in ceramide metabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within ceramide metabolic process	ISO Inferred from Sequence Orthology more info	PubMed
involved_in ceramide metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in phosphorylation	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in membrane	ISO Inferred from Sequence Orthology more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: ceramide kinase

Names: acylsphingosine kinase; mCERK

NP_663450.3

EC 2.7.1.138

XP_011243890.1

EC 2.7.1.138

XP_030104368.1

EC 2.7.1.138

XP_030104369.1

EC 2.7.1.138

XP_036015253.1

EC 2.7.1.138

XP_036015254.1

EC 2.7.1.138

XP_036015255.1

EC 2.7.1.138

XP_036015256.1

EC 2.7.1.138

XP_036015257.1

EC 2.7.1.138

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_145475.4 → NP_663450.3 ceramide kinase

See identical proteins and their annotated locations for NP_663450.3

Status: VALIDATED

Source sequence(s)

AC116764, AK042077, AK052269, BU756835

Consensus CDS

CCDS27728.1

UniProtKB/Swiss-Prot

Q52KP2, Q8K4Q7

Related

ENSMUSP00000038203.10, ENSMUST00000044332.16

Conserved Domains (1) summary

PLN02204
Location:84 → 528

PLN02204; diacylglycerol kinase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000081.7 Reference GRCm39 C57BL/6J

Range

86023302..86070537 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036159362.1 → XP_036015255.1 ceramide kinase isoform X4

UniProtKB/TrEMBL

Q6ZPK5

Related

ENSMUST00000150767.8

Conserved Domains (1) summary

cl01255
Location:7 → 451

DAGK_cat; Diacylglycerol kinase catalytic domain
XM_011245588.4 → XP_011243890.1 ceramide kinase isoform X3

Conserved Domains (1) summary

PLN02204
Location:84 → 477

PLN02204; diacylglycerol kinase
XM_030248509.2 → XP_030104369.1 ceramide kinase isoform X8

Conserved Domains (1) summary

cl01255
Location:20 → 279

DAGK_cat; Diacylglycerol kinase catalytic domain
XM_030248508.2 → XP_030104368.1 ceramide kinase isoform X7

UniProtKB/TrEMBL

Q6ZPK5

Conserved Domains (1) summary

PLN02204
Location:20 → 330

PLN02204; diacylglycerol kinase
XM_036159361.1 → XP_036015254.1 ceramide kinase isoform X2

Conserved Domains (1) summary

cl01255
Location:84 → 415

DAGK_cat; Diacylglycerol kinase catalytic domain
XM_036159363.1 → XP_036015256.1 ceramide kinase isoform X5

Conserved Domains (1) summary

cl01255
Location:20 → 268

DAGK_cat; Diacylglycerol kinase catalytic domain
XM_036159360.1 → XP_036015253.1 ceramide kinase isoform X1

Conserved Domains (1) summary

cl01255
Location:84 → 466

DAGK_cat; Diacylglycerol kinase catalytic domain
XM_036159364.1 → XP_036015257.1 ceramide kinase isoform X6

Conserved Domains (1) summary

cl01255
Location:84 → 331

DAGK_cat; Diacylglycerol kinase catalytic domain