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Uba3 ubiquitin-like modifier activating enzyme 3 [ Mus musculus (house mouse) ]

Gene ID: 22200, updated on 21-Apr-2024

Summary

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Official Symbol
Uba3provided by MGI
Official Full Name
ubiquitin-like modifier activating enzyme 3provided by MGI
Primary source
MGI:MGI:1341217
See related
Ensembl:ENSMUSG00000030061 AllianceGenome:MGI:1341217
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Ube1c; A830034N06Rik
Summary
The protein encoded by this gene is the catalytic subunit of the enzyme that activates NEDD8, a ubiquitin-like molecule that binds to its target proteins through an enzymatic reaction analagous to ubiquitylation. Embryonic mice deficient for this protein die prior to implantation and display apoptosis of the inner cell mass. Trophoblastic cells cannot enter S phase, demonstrating that this gene is required for cell cycle progression during embryogenesis. Two pseudogenes have been found for this gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]
Expression
Broad expression in CNS E11.5 (RPKM 17.1), placenta adult (RPKM 15.3) and 24 other tissues See more
Orthologs
human all
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Genomic context

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Location:
6 D3; 6 44.9 cM
Exon count:
19
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (97160593..97182608, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (97183632..97205647, complement)

Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene EGF domain specific O-linked N-acetylglucosamine transferase Neighboring gene STARR-seq mESC enhancer starr_16775 Neighboring gene STARR-seq mESC enhancer starr_16776 Neighboring gene TATA element modulatory factor 1 Neighboring gene predicted gene, 26175 Neighboring gene STARR-positive B cell enhancer ABC_E2796 Neighboring gene STARR-seq mESC enhancer starr_16779 Neighboring gene STARR-positive B cell enhancer ABC_E1324 Neighboring gene STARR-seq mESC enhancer starr_16780 Neighboring gene ADP-ribosylation factor-like 6 interacting protein 5 Neighboring gene leiomodin 3 (fetal) Neighboring gene predicted gene, 32191

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Myeloid neddylation targets IRF7 and promotes host innate immunity against RNA viruses. Zhao M, et al. PLoS Pathog, 2021 Sep. PMID 34506605, Free PMC Article
  2. Neddylation contributes to CD4+ T cell-mediated protective immunity against blood-stage Plasmodium infection. Cheng Q, et al. PLoS Pathog, 2018 Nov. PMID 30462731, Free PMC Article
  3. The cell cycle of early mammalian embryos: lessons from genetic mouse models. Artus J, et al. Cell Cycle, 2006 Mar. PMID 16481745
  4. Neddylation is required for herpes simplex virus type I (HSV-1)-induced early phase interferon-beta production. Zhang X, et al. Cell Mol Immunol, 2016 Sep. PMID 27593482, Free PMC Article
  5. Hepatic neddylation targets and stabilizes electron transfer flavoproteins to facilitate fatty acid β-oxidation. Zhang X, et al. Proc Natl Acad Sci U S A, 2020 Feb 4. PMID 31941714, Free PMC Article

See all (27) citations in PubMed

GeneRIFs: Gene References Into Functions

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Transgenic (1) 
  • Gene trapped (1) 
  • Targeted (2)  1 citation
  • Endonuclease-mediated (1) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables NEDD8 activating enzyme activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables NEDD8 activating enzyme activity IDA
Inferred from Direct Assay
more info
 PubMed 
contributes_to NEDD8 activating enzyme activity ISO
Inferred from Sequence Orthology
more info
  
enables NEDD8 activating enzyme activity ISO
Inferred from Sequence Orthology
more info
 PubMed 
enables NEDD8 activating enzyme activity TAS
Traceable Author Statement
more info
 PubMed 
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables nuclear receptor binding ISO
Inferred from Sequence Orthology
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein heterodimerization activity ISO
Inferred from Sequence Orthology
more info
  
enables protein-containing complex binding ISO
Inferred from Sequence Orthology
more info
  
enables ubiquitin-like modifier activating enzyme activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within cell cycle IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within endomitotic cell cycle IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within mitotic cell cycle IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of DNA-templated transcription ISO
Inferred from Sequence Orthology
more info
  
involved_in protein neddylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein neddylation ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within regulation of cell cycle IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  
part_of protein-containing complex ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
NEDD8-activating enzyme E1 catalytic subunit
Names
NEDD8-activating enzyme E1C
ubiquitin-activating enzyme 3
ubiquitin-activating enzyme E1C
NP_001104576.1
NP_001288786.1
NP_001288787.1
NP_001288788.1
NP_035796.2
XP_011239592.1
XP_030111175.1
XP_030111176.1
XP_036021915.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001111106.2 → NP_001104576.1  NEDD8-activating enzyme E1 catalytic subunit isoform 2

    See identical proteins and their annotated locations for NP_001104576.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1.
    Source sequence(s)
    AK159381, AK166655, BY639644
    Consensus CDS
    CCDS51859.1
    UniProtKB/TrEMBL
    Q3TL72, Q66JT6
    Related
    ENSMUSP00000130954.2, ENSMUST00000164744.8
    Conserved Domains (3) summary
    cd01488
    Location:57 → 354
    Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
    pfam00899
    Location:53 → 352
    ThiF; ThiF family
    pfam08825
    Location:362 → 447
    E2_bind; E2 binding domain

  2. NM_001301857.1 → NP_001288786.1  NEDD8-activating enzyme E1 catalytic subunit isoform 3

    See identical proteins and their annotated locations for NP_001288786.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) uses an alternate splice site in the 5' coding region and uses a downstream start codon compared to variant 1. It encodes isoform 3 which has a shorter N-terminus compared to isoform 1.
    Source sequence(s)
    AK032514, AK159381, AK166655, BY639644
    UniProtKB/TrEMBL
    Q66JT6
    Conserved Domains (2) summary
    cd01488
    Location:50 → 347
    Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
    pfam08825
    Location:355 → 440
    E2_bind; E2 binding domain

  3. NM_001301858.1 → NP_001288787.1  NEDD8-activating enzyme E1 catalytic subunit isoform 4

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes an isoform (4) that is shorter compared to isoform 1.
    Source sequence(s)
    AK159381, AK166655, BC080776, BY639644
    UniProtKB/TrEMBL
    Q66JT6
    Conserved Domains (3) summary
    cd01488
    Location:62 → 341
    Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
    pfam00899
    Location:62 → 339
    ThiF; ThiF family
    pfam08825
    Location:349 → 434
    E2_bind; E2 binding domain

  4. NM_001301859.1 → NP_001288788.1  NEDD8-activating enzyme E1 catalytic subunit isoform 5

    Status: REVIEWED

    Description
    Transcript Variant: This variant (5) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 5 which is shorter and has a distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AK014433, AK159381, CF928750
    UniProtKB/TrEMBL
    Q66JT6
    Conserved Domains (2) summary
    cd01488
    Location:71 → 368
    Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
    pfam00899
    Location:67 → 366
    ThiF; ThiF family

  5. NM_011666.3 → NP_035796.2  NEDD8-activating enzyme E1 catalytic subunit isoform 1

    See identical proteins and their annotated locations for NP_035796.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (1).
    Source sequence(s)
    AK159381, AK166655, BY639644
    Consensus CDS
    CCDS51860.1
    UniProtKB/Swiss-Prot
    O88598, Q3TG68, Q8C878, Q9D6B7
    UniProtKB/TrEMBL
    Q66JT6
    Related
    ENSMUSP00000086701.6, ENSMUST00000089287.7
    Conserved Domains (3) summary
    cd01488
    Location:71 → 368
    Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
    pfam00899
    Location:67 → 366
    ThiF; ThiF family
    pfam08825
    Location:376 → 461
    E2_bind; E2 binding domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000072.7 Reference GRCm39 C57BL/6J

    Range
    97160593..97182608 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_030255316.2 → XP_030111176.1  NEDD8-activating enzyme E1 catalytic subunit isoform X2

    UniProtKB/TrEMBL
    Q66JT6
    Conserved Domains (2) summary
    cd01488
    Location:48 → 327
    Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
    pfam08825
    Location:335 → 420
    E2_bind; E2 binding domain

  2. XM_030255315.2 → XP_030111175.1  NEDD8-activating enzyme E1 catalytic subunit isoform X1

    UniProtKB/TrEMBL
    Q66JT6
    Conserved Domains (2) summary
    cd01488
    Location:50 → 347
    Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
    pfam08825
    Location:355 → 440
    E2_bind; E2 binding domain

  3. XM_036166022.1 → XP_036021915.1  NEDD8-activating enzyme E1 catalytic subunit isoform X1

    UniProtKB/TrEMBL
    Q66JT6
    Conserved Domains (2) summary
    cd01488
    Location:50 → 347
    Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
    pfam08825
    Location:355 → 440
    E2_bind; E2 binding domain

  4. XM_011241290.4 → XP_011239592.1  NEDD8-activating enzyme E1 catalytic subunit isoform X1

    See identical proteins and their annotated locations for XP_011239592.1

    UniProtKB/TrEMBL
    Q66JT6
    Conserved Domains (2) summary
    cd01488
    Location:50 → 347
    Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
    pfam08825
    Location:355 → 440
    E2_bind; E2 binding domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8C878.2 GenPept UniProtKB/Swiss-Prot:Q8C878

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