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Tk1 thymidine kinase 1 [ Mus musculus (house mouse) ]

Gene ID: 21877, updated on 5-Mar-2024

Summary

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Official Symbol
Tk1provided by MGI
Official Full Name
thymidine kinase 1provided by MGI
Primary source
MGI:MGI:98763
See related
Ensembl:ENSMUSG00000025574 AllianceGenome:MGI:98763
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Tk-1; Tk1a; Tk1b; D530002A18Rik
Summary
Enables thymidine kinase activity. Predicted to be involved in protein homotetramerization and thymidine metabolic process. Predicted to act upstream of or within DNA biosynthetic process. Predicted to be located in cytosol. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; heart; and hemolymphoid system. Orthologous to human TK1 (thymidine kinase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in liver E14.5 (RPKM 50.7), liver E14 (RPKM 47.2) and 19 other tissues See more
Orthologs
human all
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Genomic context

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Location:
11 E2; 11 82.96 cM
Exon count:
9
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 11 NC_000077.7 (117706345..117716913, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 11 NC_000077.6 (117815519..117826087, complement)

Chromosome 11 - NC_000077.7Genomic Context describing neighboring genes Neighboring gene RIKEN cDNA 6030468B19 gene Neighboring gene STARR-positive B cell enhancer ABC_E5305 Neighboring gene STARR-positive B cell enhancer ABC_E5306 Neighboring gene synaptogyrin 2 Neighboring gene STARR-positive B cell enhancer ABC_E11501 Neighboring gene STARR-positive B cell enhancer ABC_E4020 Neighboring gene CapStarr-seq enhancer MGSCv37_chr11:117693509-117693710 Neighboring gene arylformamidase Neighboring gene CapStarr-seq enhancer MGSCv37_chr11:117693767-117693876 Neighboring gene CapStarr-seq enhancer MGSCv37_chr11:117710178-117710379 Neighboring gene baculoviral IAP repeat-containing 5 Neighboring gene VISTA enhancer mm780 Neighboring gene transmembrane protein 235

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Micronucleated erythrocyte frequency in control and azidothymidine-treated Tk+/+, Tk+/- and Tk-/- mice. Dobrovolsky VN, et al. Mutat Res, 2005 Mar 1. PMID 15708581
  2. Analysis of mutations in the Tk gene of Tk(+/-) mice treated as neonates with 3'-azido-3'-deoxythymidine (AZT). Mittelstaedt RA, et al. Mutat Res, 2004 Mar 22. PMID 15013700
  3. Mice deficient for cytosolic thymidine kinase gene develop fatal kidney disease. Dobrovolsky VN, et al. Mol Genet Metab, 2003 Jan. PMID 12559842
  4. Mutant frequencies and loss of heterozygosity induced by N-ethyl-N-nitrosourea in the thymidine kinase gene of L5178Y/TK(+/-)-3.7.2C mouse lymphoma cells. Chen T, et al. Mutagenesis, 2002 Mar. PMID 11880538
  5. Development of a novel mouse tk+/- embryonic stem cell line for use in mutagenicity studies. Dobrovolsky VN, et al. Environ Mol Mutagen, 1996. PMID 8991081

See all (81) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Transcription factor Dp-1 knockdown downregulates thymidine kinase 1 expression to protect against proliferation and epithelial-mesenchymal transition in cervical cancer.
    Title: Transcription factor Dp-1 knockdown downregulates thymidine kinase 1 expression to protect against proliferation and epithelial-mesenchymal transition in cervical cancer.
  2. The down-regulation of Tk1 activity unmasks Tk2 deficiency in Tk2-/- mice and correlates with the onset of mtDNA depletion in the brain and the heart.
    Title: Onset and organ specificity of Tk2 deficiency depends on Tk1 down-regulation and transcriptional compensation.
  3. blood cells are less dependent on mitochondrial Tk2 compared with several other tissues; these cells can synthesize deoxyribonucleotides required for mtDNA replication by alternative pathways such as phosphorylation of thymidine by cytosolic Tk1
    Title: Hematopoiesis in the thymidine kinase 2 deficient mouse model of mitochondrial DNA depletion syndrome.
  4. Mutations in the TK1 gene were studied in neonatal transgenic mice treated with AZT.
    Title: Analysis of mutations in the Tk gene of Tk(+/-) mice treated as neonates with 3'-azido-3'-deoxythymidine (AZT).
  5. the small colony mutant phenotype in L5178Y lymphoma is not caused by deletion of both Trp53 and thymidine kinase 1
    Title: Loss of P53 heterozygosity is not responsible for the small colony thymidine kinase mutant phenotype in L5178Y mouse lymphoma cells.
  6. Alterations in Tk KO mice indicate that the pyrimidine nucleotide salvage pathway is indispensable in vivo.
    Title: Mice deficient for cytosolic thymidine kinase gene develop fatal kidney disease.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Gene trapped (1) 
  • Targeted (1)  1 citation
  • Endonuclease-mediated (3) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables thymidine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables thymidine kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables thymidine kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within DNA biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA synthesis involved in mitotic DNA replication ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within deoxyribonucleoside monophosphate biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within nucleotide biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within phosphorylation IEA
Inferred from Electronic Annotation
more info
  
involved_in protein homotetramerization ISO
Inferred from Sequence Orthology
more info
  
involved_in thymidine biosynthetic process ISO
Inferred from Sequence Orthology
more info
  
involved_in thymidine metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in thymidine metabolic process ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol TAS
Traceable Author Statement
more info
  

General protein information

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Preferred Names
thymidine kinase, cytosolic
NP_001258658.1
NP_033413.2
XP_006533212.1
XP_036012517.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001271729.1 → NP_001258658.1  thymidine kinase, cytosolic isoform 2

    See identical proteins and their annotated locations for NP_001258658.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) lacks an alternate coding exon compared to variant 1, that causes a frameshift and use of a downstream translation start site compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.
    Source sequence(s)
    AA047942, AK139244, BY773001, CF732600
    UniProtKB/Swiss-Prot
    P04184
    Conserved Domains (1) summary
    cl23762
    Location:1 → 161
    TK; Thymidine kinase

  2. NM_009387.2 → NP_033413.2  thymidine kinase, cytosolic isoform 1

    See identical proteins and their annotated locations for NP_033413.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AA047942, AK139244, BY773001
    Consensus CDS
    CCDS25692.1
    UniProtKB/Swiss-Prot
    P04184, Q58EU0, Q8C3P8, Q921J8
    UniProtKB/TrEMBL
    A0A1S6GWJ3
    Related
    ENSMUSP00000026661.4, ENSMUST00000026661.4
    Conserved Domains (1) summary
    cl23762
    Location:18 → 197
    TK; Thymidine kinase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000077.7 Reference GRCm39 C57BL/6J

    Range
    117706345..117716913 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036156624.1 → XP_036012517.1  thymidine kinase, cytosolic isoform X2

    Conserved Domains (1) summary
    cl23762
    Location:1 → 161
    TK; Thymidine kinase

  2. XM_006533149.3 → XP_006533212.1  thymidine kinase, cytosolic isoform X1

    Conserved Domains (1) summary
    cl23762
    Location:41 → 199
    TK; Thymidine kinase

RNA

  1. XR_004936934.1 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P04184.3 GenPept UniProtKB/Swiss-Prot:P04184

Gene LinkOut

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