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G2e3 G2/M-phase specific E3 ubiquitin ligase [ Mus musculus (house mouse) ]

Gene ID: 217558, updated on 11-Apr-2024

Summary

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Official Symbol
G2e3provided by MGI
Official Full Name
G2/M-phase specific E3 ubiquitin ligaseprovided by MGI
Primary source
MGI:MGI:2444298
See related
Ensembl:ENSMUSG00000035293 AllianceGenome:MGI:2444298
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
mKIAA1333; 9030416F18; 6030408C04Rik; D930034K21Rik
Summary
Enables ubiquitin protein ligase activity. Acts upstream of or within blastocyst development; negative regulation of intrinsic apoptotic signaling pathway; and protein polyubiquitination. Predicted to be located in Golgi apparatus and cytosol. Predicted to be active in nucleus. Is expressed in several structures, including central nervous system; epithelium; limb; liver; and male reproductive gland or organ. Orthologous to human G2E3 (G2/M-phase specific E3 ubiquitin protein ligase). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in CNS E11.5 (RPKM 7.7), CNS E14 (RPKM 6.0) and 17 other tissues See more
Orthologs
human all
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Genomic context

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See G2e3 in Genome Data Viewer
Location:
12 B3; 12 22.11 cM
Exon count:
16
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 12 NC_000078.7 (51395013..51423769)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 12 NC_000078.6 (51348230..51376986)

Chromosome 12 - NC_000078.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 34745 Neighboring gene tubulin, alpha 1B pseudogene Neighboring gene STARR-seq mESC enhancer starr_32098 Neighboring gene STARR-seq mESC enhancer starr_32099 Neighboring gene STARR-seq mESC enhancer starr_32100 Neighboring gene ribosomal protein S11, pseudogene 4 Neighboring gene STARR-seq mESC enhancer starr_32102 Neighboring gene STARR-seq mESC enhancer starr_32103 Neighboring gene predicted gene, 40422 Neighboring gene Sec1 family domain containing 1 Neighboring gene high mobility group protein 1 pseudogene Neighboring gene zyg-11 homolog A pseudogene

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Obesity of G2e3 Knockout Mice Suggests That Obesity-Associated Variants Near Human G2E3 Decrease G2E3 Activity. Powell DR, et al. Diabetes Metab Syndr Obes, 2020. PMID 32801815, Free PMC Article
  2. G2E3 is a dual function ubiquitin ligase required for early embryonic development. Brooks WS, et al. J Biol Chem, 2008 Aug 8. PMID 18511420, Free PMC Article
  3. A genomic atlas of mouse hypothalamic development. Shimogori T, et al. Nat Neurosci, 2010 Jun. PMID 20436479, Free PMC Article
  4. Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries. Okazaki N, et al. DNA Res, 2003 Aug 31. PMID 14621295
  5. Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method. Piao Y, et al. Genome Res, 2001 Sep. PMID 11544199, Free PMC Article

See all (24) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. G2E3 is a dual function ubiquitin ligase essential for prevention of apoptosis in early embryogenesis
    Title: G2E3 is a dual function ubiquitin ligase required for early embryonic development.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Gene trapped (2)  1 citation
  • Endonuclease-mediated (2) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ubiquitin-protein transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within apoptotic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within blastocyst development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within multicellular organism development IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within negative regulation of intrinsic apoptotic signaling pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within protein polyubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in Golgi apparatus ISO
Inferred from Sequence Orthology
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in intracellular membrane-bounded organelle ISO
Inferred from Sequence Orthology
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
G2/M phase-specific E3 ubiquitin-protein ligase
Names
G2/M phase-specific HECT-type E3 ubiquitin transferase
NP_001015099.2
NP_001161435.1
NP_001161436.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001015099.2 → NP_001015099.2  G2/M phase-specific E3 ubiquitin-protein ligase isoform 1

    See identical proteins and their annotated locations for NP_001015099.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1.
    Source sequence(s)
    AA231622, AK086526, AK129332, BY077085, CJ239277
    Consensus CDS
    CCDS25900.1
    UniProtKB/Swiss-Prot
    E9QK24, Q5RJY2, Q6ZPT7, Q8BNA4
    Related
    ENSMUSP00000054474.7, ENSMUST00000054308.13
    Conserved Domains (4) summary
    smart00119
    Location:388 → 690
    HECTc; Domain Homologous to E6-AP Carboxyl Terminus with
    cd15496
    Location:232 → 285
    PHD_PHF7_G2E3_like; PHD finger found in PHD finger protein 7 (PHF7) and G2/M phase-specific E3 ubiquitin-protein ligase (G2E3)
    cd15669
    Location:14 → 127
    ePHD_PHF7_G2E3_like; Extended PHD finger found in PHD finger protein 7 (PHF7) and G2/M phase-specific E3 ubiquitin-protein ligase (G2E3)
    cl00077
    Location:365 → 681
    HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

  2. NM_001167963.1 → NP_001161435.1  G2/M phase-specific E3 ubiquitin-protein ligase isoform 2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) contains an additional in-frame coding exon compared to variant 1, resulting in a longer isoform (2) containing a 23 aa protein segment not found in isoform 1.
    Source sequence(s)
    AA231622, AK033513, AK086526, AK129332, BY077085, CJ239277
    Consensus CDS
    CCDS49061.1
    UniProtKB/TrEMBL
    E9Q7C2
    Related
    ENSMUSP00000113270.2, ENSMUST00000119211.8
    Conserved Domains (4) summary
    smart00119
    Location:411 → 713
    HECTc; Domain Homologous to E6-AP Carboxyl Terminus with
    cd15496
    Location:232 → 285
    PHD_PHF7_G2E3_like; PHD finger found in PHD finger protein 7 (PHF7) and G2/M phase-specific E3 ubiquitin-protein ligase (G2E3)
    cd15669
    Location:14 → 127
    ePHD_PHF7_G2E3_like; Extended PHD finger found in PHD finger protein 7 (PHF7) and G2/M phase-specific E3 ubiquitin-protein ligase (G2E3)
    cl00077
    Location:388 → 704
    HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

  3. NM_001167964.1 → NP_001161436.1  G2/M phase-specific E3 ubiquitin-protein ligase isoform 3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) is missing an internal coding exon and uses an alternate acceptor splice site at another coding exon compared to variant 1. However, it retains the reading frame and encodes a shorter isoform (3) lacking an internal protein segment compared to isoform 1.
    Source sequence(s)
    AA231622, AK031333, AK086526, AK129332, BY077085, CJ239277
    Consensus CDS
    CCDS49062.1
    UniProtKB/TrEMBL
    E9Q7E5
    Related
    ENSMUSP00000113191.2, ENSMUST00000121521.2
    Conserved Domains (3) summary
    cd15496
    Location:232 → 285
    PHD_PHF7_G2E3_like; PHD finger found in PHD finger protein 7 (PHF7) and G2/M phase-specific E3 ubiquitin-protein ligase (G2E3)
    cd15669
    Location:14 → 127
    ePHD_PHF7_G2E3_like; Extended PHD finger found in PHD finger protein 7 (PHF7) and G2/M phase-specific E3 ubiquitin-protein ligase (G2E3)
    cl00077
    Location:346 → 596
    HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000078.7 Reference GRCm39 C57BL/6J

    Range
    51395013..51423769
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q5RJY2.2 GenPept UniProtKB/Swiss-Prot:Q5RJY2

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