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Engase endo-beta-N-acetylglucosaminidase [ Mus musculus (house mouse) ]

Gene ID: 217364, updated on 3-Apr-2024

Summary

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Official Symbol
Engaseprovided by MGI
Official Full Name
endo-beta-N-acetylglucosaminidaseprovided by MGI
Primary source
MGI:MGI:2443788
See related
Ensembl:ENSMUSG00000033857 AllianceGenome:MGI:2443788
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
C130099A03; D230014K01Rik
Summary
Predicted to enable hydrolase activity, hydrolyzing O-glycosyl compounds. Acts upstream of or within protein deglycosylation. Predicted to be located in cytoplasm. Orthologous to human ENGASE (endo-beta-N-acetylglucosaminidase). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in colon adult (RPKM 10.6), thymus adult (RPKM 10.2) and 28 other tissues See more
Orthologs
human all
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Genomic context

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Location:
11 E2; 11 83.21 cM
Exon count:
14
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 11 NC_000077.7 (118367635..118380038)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 11 NC_000077.6 (118476802..118489209)

Chromosome 11 - NC_000077.7Genomic Context describing neighboring genes Neighboring gene C1q and tumor necrosis factor related protein 1 Neighboring gene STARR-seq mESC enhancer starr_31303 Neighboring gene STARR-positive B cell enhancer ABC_E3042 Neighboring gene predicted gene 11747 Neighboring gene ribosomal protein L18 pseudogene Neighboring gene RNA binding protein, fox-1 homolog (C. elegans) 3 Neighboring gene predicted gene, 53655 Neighboring gene predicted gene, 53656

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Cytosolic N-GlcNAc proteins are formed by the action of endo-β-N-acetylglucosaminidase. Maynard JC, et al. Biochem Biophys Res Commun, 2020 Oct 1. PMID 32782141, Free PMC Article
  2. Non-lysosomal Degradation of Singly Phosphorylated Oligosaccharides Initiated by the Action of a Cytosolic Endo-β-N-acetylglucosaminidase. Harada Y, et al. J Biol Chem, 2016 Apr 8. PMID 26858256, Free PMC Article
  3. Lethality of mice bearing a knockout of the Ngly1-gene is partially rescued by the additional deletion of the Engase gene. Fujihira H, et al. PLoS Genet, 2017 Apr. PMID 28426790, Free PMC Article
  4. Liver-specific deletion of Ngly1 causes abnormal nuclear morphology and lipid metabolism under food stress. Fujihira H, et al. Biochim Biophys Acta Mol Basis Dis, 2020 Mar 1. PMID 31733337
  5. Endo-β-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells. Huang C, et al. Proc Natl Acad Sci U S A, 2015 Feb 3. PMID 25605922, Free PMC Article

See all (19) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Cytosolic N-GlcNAc proteins are formed by the action of endo-beta-N-acetylglucosaminidase.
    Title: Cytosolic N-GlcNAc proteins are formed by the action of endo-β-N-acetylglucosaminidase.
  2. These observations strongly suggest that the Ngly1- or Ngly1/Engase-deficient mice could serve as a valuable animal model for studies related to the pathogenesis of the NGLY1-deficiency, and that cytoplasmic ENGase represents one of the potential therapeutic targets for this genetic disorder.
    Title: Lethality of mice bearing a knockout of the Ngly1-gene is partially rescued by the additional deletion of the Engase gene.
  3. ENGase efficiently hydrolyzes phosphorylated oligosaccharides that are larger than Man4GlcNAc2-P, generating GlcNAc-1-P and neutral Gn1-type free oligosaccharides.
    Title: Non-lysosomal Degradation of Singly Phosphorylated Oligosaccharides Initiated by the Action of a Cytosolic Endo-β-N-acetylglucosaminidase.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (1)  1 citation
  • Endonuclease-mediated (3) 

Pathways from PubChem

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General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, acting on glycosyl bonds IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, hydrolyzing O-glycosyl compounds IBA
Inferred from Biological aspect of Ancestor
more info
  
enables mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in protein deglycosylation IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within protein deglycosylation IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
cytosolic endo-beta-N-acetylglucosaminidase
NP_001346297.1
NP_001390767.1
NP_001390768.1
NP_001390769.1
NP_001390770.1
NP_766161.1
XP_006533194.1
XP_006533196.1
XP_030101770.1
XP_036012510.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001359368.1 → NP_001346297.1  cytosolic endo-beta-N-acetylglucosaminidase isoform 2

    Status: VALIDATED

    Source sequence(s)
    AK048667, BC060640, BY070749
    Conserved Domains (1) summary
    cd06547
    Location:27 → 337
    GH85_ENGase; Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the ...

  2. NM_001403838.1 → NP_001390767.1  cytosolic endo-beta-N-acetylglucosaminidase isoform 3

    Status: VALIDATED

    Source sequence(s)
    AL591075

  3. NM_001403839.1 → NP_001390768.1  cytosolic endo-beta-N-acetylglucosaminidase isoform 2

    Status: VALIDATED

    Source sequence(s)
    AL591075

  4. NM_001403840.1 → NP_001390769.1  cytosolic endo-beta-N-acetylglucosaminidase isoform 4

    Status: VALIDATED

    Source sequence(s)
    AL591075

  5. NM_001403841.1 → NP_001390770.1  cytosolic endo-beta-N-acetylglucosaminidase isoform 5

    Status: VALIDATED

    Source sequence(s)
    AL591075

  6. NM_172573.3 → NP_766161.1  cytosolic endo-beta-N-acetylglucosaminidase isoform 1

    See identical proteins and their annotated locations for NP_766161.1

    Status: VALIDATED

    Source sequence(s)
    AK048667, BC060640, BY070749
    Consensus CDS
    CCDS25704.1
    UniProtKB/Swiss-Prot
    Q3TK58, Q8BJF1, Q8BX80
    Related
    ENSMUSP00000117538.3, ENSMUST00000135383.9
    Conserved Domains (1) summary
    cd06547
    Location:118 → 428
    GH85_ENGase; Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the ...

RNA

  1. NR_153103.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AK048667, AK084254, BC060640, BY070749

  2. NR_175777.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AL591075
    Related
    ENSMUST00000171769.8

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000077.7 Reference GRCm39 C57BL/6J

    Range
    118367635..118380038
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036156617.1 → XP_036012510.1  cytosolic endo-beta-N-acetylglucosaminidase isoform X3

    Conserved Domains (1) summary
    cd06547
    Location:118 → 428
    GH85_ENGase; Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the ...

  2. XM_030245910.2 → XP_030101770.1  cytosolic endo-beta-N-acetylglucosaminidase isoform X1

    Conserved Domains (1) summary
    cd06547
    Location:27 → 337
    GH85_ENGase; Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the ...

  3. XM_006533131.5 → XP_006533194.1  cytosolic endo-beta-N-acetylglucosaminidase isoform X1

    Conserved Domains (1) summary
    cd06547
    Location:27 → 337
    GH85_ENGase; Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the ...

  4. XM_006533133.4 → XP_006533196.1  cytosolic endo-beta-N-acetylglucosaminidase isoform X5

    UniProtKB/TrEMBL
    F7CXI4
    Related
    ENSMUSP00000125886.2, ENSMUST00000168689.2
    Conserved Domains (1) summary
    cl10447
    Location:3 → 123
    GH18_chitinase-like; The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have ...

RNA

  1. XR_003949406.2 RNA Sequence

  2. XR_004936933.1 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8BX80.1 GenPept UniProtKB/Swiss-Prot:Q8BX80

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