Ube2o ubiquitin-conjugating enzyme E2O [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ube2oprovided by MGI
Official Full Name: ubiquitin-conjugating enzyme E2Oprovided by MGI
Primary source: MGI:MGI:2444266
See related: Ensembl:ENSMUSG00000020802 AllianceGenome:MGI:2444266
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: E2-230K; mKIAA1734; 9630022H21; B230113M03Rik
Summary: Predicted to enable cysteine-type endopeptidase inhibitor activity; ubiquitin conjugating enzyme activity; and ubiquitin protein ligase activity. Predicted to be involved in several processes, including positive regulation of BMP signaling pathway; protein ubiquitination; and retrograde transport, endosome to Golgi. Predicted to be located in cytoplasm and nuclear body. Predicted to be active in nucleus. Is expressed in several structures, including alimentary system; central nervous system; connective tissue; genitourinary system; and immune system. Orthologous to human UBE2O (ubiquitin conjugating enzyme E2 O). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in spleen adult (RPKM 21.5), liver E14.5 (RPKM 19.9) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 E2; 11 81.42 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (116428500..116472281, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (116537740..116581503, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Age-Associated UBE2O Reduction Promotes Neuronal Death in Alzheimer's Disease.
Title: Age-Associated UBE2O Reduction Promotes Neuronal Death in Alzheimer's Disease.
Over expression of ubiquitin-conjugating enzyme E2O in bone marrow mesenchymal stromal cells partially attenuates acute myeloid leukaemia progression.
Title: Over expression of ubiquitin-conjugating enzyme E2O in bone marrow mesenchymal stromal cells partially attenuates acute myeloid leukaemia progression.
UBE2O promotes lipid metabolic reprogramming and liver cancer progression by mediating HADHA ubiquitination.
Title: UBE2O promotes lipid metabolic reprogramming and liver cancer progression by mediating HADHA ubiquitination.
Through phenotype analysis of high-fat diet-induced obesity and metabolic syndrome mice with muscle-, fat-, or liver-specific knockout of Ube2o, study identified Ube2o as an essential regulator of glucose and lipid metabolism programs in skeletal muscle, but not in adipose or liver tissue. Mechanistically, Ube2o acted as a ubiquitin ligase and targeted AMPKalpha2 for ubiquitin-dependent degradation in skeletal muscles.
Title: A muscle-specific UBE2O/AMPKα2 axis promotes insulin resistance and metabolic syndrome in obesity.
This work identifies UBE2O as a critical regulator in the ubiquitin-proteasome system, which modulates BMAL1 transcriptional activity and circadian function by promoting BMAL1 ubiquitination and degradation under normal physiological conditions.
Title: Ubiquitin-conjugating enzyme UBE2O regulates cellular clock function by promoting the degradation of the transcription factor BMAL1.
UBE2O recognized ribosomal proteins and other substrates directly, targeting them to proteasomes for degradation.
Title: UBE2O remodels the proteome during terminal erythroid differentiation.
monoubiquitinated SMAD6 impairs the binding affinity of non-modified SMAD6 to the BMP type I receptor. Moreover, UBE2O and SMAD6 cooperated in the regulation of BMP7-induced adipogenesis
Title: Fine-tuning BMP7 signalling in adipogenesis by UBE2O/E2-230K-mediated monoubiquitination of SMAD6.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (5) 1 citation
Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin conjugating enzyme activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in positive regulation of BMP signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in protein K63-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein monoubiquitination	ISO Inferred from Sequence Orthology more info
involved_in retrograde transport, endosome to Golgi	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in nuclear body	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O

Names: E2/E3 hybrid ubiquitin-protein ligase UBE2O; ubiquitin carrier protein O; ubiquitin-conjugating enzyme E2 of 230 kDa; ubiquitin-conjugating enzyme E2-230K; ubiquitin-protein ligase O; ubiquitinating enzyme E2-230 kDa

NP_001414250.1

EC 2.3.2.24

NP_776116.2

EC 2.3.2.24

XP_011247262.1

EC 2.3.2.24

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001427321.1 → NP_001414250.1 (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O isoform 2

Status: VALIDATED

Source sequence(s)

AL607039, AL645851
NM_173755.4 → NP_776116.2 (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O isoform 1

See identical proteins and their annotated locations for NP_776116.2

Status: VALIDATED

Source sequence(s)

AL607039, AL645851

Consensus CDS

CCDS25670.1

UniProtKB/Swiss-Prot

A2A7X3, Q60800, Q6PCR9, Q6ZPJ3, Q7TPN2, Q8BLE8

Related

ENSMUSP00000080791.5, ENSMUST00000082152.5

Conserved Domains (1) summary

cd00195
Location:955 → 1105

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

116428500..116472281 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011248960.1 → XP_011247262.1 (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O isoform X2

Related

ENSMUST00000134102.2

Conserved Domains (1) summary

cd00195
Location:398 → 548

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...