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Med16 mediator complex subunit 16 [ Mus musculus (house mouse) ]

Gene ID: 216154, updated on 3-Apr-2024

Summary

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Official Symbol
Med16provided by MGI
Official Full Name
mediator complex subunit 16provided by MGI
Primary source
MGI:MGI:2158394
See related
Ensembl:ENSMUSG00000013833 AllianceGenome:MGI:2158394
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
95kDa; DRIP92; Thrap5; Trap95; A630083L04
Summary
Predicted to enable thyroid hormone receptor binding activity and transcription coactivator activity. Predicted to be involved in positive regulation of transcription initiation from RNA polymerase II promoter. Predicted to act upstream of or within transcription by RNA polymerase II. Part of mediator complex. Is expressed in cap mesenchyme; limb mesenchyme; renal interstitium; and testis. Orthologous to human MED16 (mediator complex subunit 16). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in testis adult (RPKM 63.2), ovary adult (RPKM 52.5) and 28 other tissues See more
Orthologs
human all
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Genomic context

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See Med16 in Genome Data Viewer
Location:
10 C1; 10 39.72 cM
Exon count:
16
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 10 NC_000076.7 (79730543..79744757, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 10 NC_000076.6 (79894706..79908938, complement)

Chromosome 10 - NC_000076.7Genomic Context describing neighboring genes Neighboring gene 40S ribosomal protein S21 pseudogene Neighboring gene complement factor D Neighboring gene STARR-positive B cell enhancer ABC_E2946 Neighboring gene STARR-positive B cell enhancer ABC_E252 Neighboring gene predicted gene, 24019 Neighboring gene R3H domain containing 4

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. The Mediator Subunit MED16 Transduces NRF2-Activating Signals into Antioxidant Gene Expression. Sekine H, et al. Mol Cell Biol, 2016 Feb 1. PMID 26572828, Free PMC Article
  2. The TRAP100 component of the TRAP/Mediator complex is essential in broad transcriptional events and development. Ito M, et al. EMBO J, 2002 Jul 1. PMID 12093747, Free PMC Article
  3. Mediator complex proximal Tail subunit MED30 is critical for Mediator core stability and cardiomyocyte transcriptional network. Tan C, et al. PLoS Genet, 2021 Sep. PMID 34506481, Free PMC Article
  4. Structural and functional organization of TRAP220, the TRAP/mediator subunit that is targeted by nuclear receptors. Malik S, et al. Mol Cell Biol, 2004 Sep. PMID 15340084, Free PMC Article
  5. Diversification of stem cell molecular repertoire by alternative splicing. Pritsker M, et al. Proc Natl Acad Sci U S A, 2005 Oct 4. PMID 16183747, Free PMC Article

See all (27) citations in PubMed

GeneRIFs: Gene References Into Functions

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (2) 
  • Endonuclease-mediated (2) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables nuclear thyroid hormone receptor binding ISO
Inferred from Sequence Orthology
more info
  
enables transcription coactivator activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in RNA polymerase II preinitiation complex assembly NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in positive regulation of DNA-templated transcription IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in positive regulation of DNA-templated transcription ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of transcription elongation by RNA polymerase II NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in positive regulation of transcription initiation by RNA polymerase II ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of transcription initiation by RNA polymerase II NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within transcription by RNA polymerase II IC
Inferred by Curator
more info
 PubMed 
Component Evidence Code Pubs
part_of core mediator complex ISO
Inferred from Sequence Orthology
more info
  
part_of mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of mediator complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of mediator complex ISO
Inferred from Sequence Orthology
more info
  
located_in nucleoplasm TAS
Traceable Author Statement
more info
  
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
mediator of RNA polymerase II transcription subunit 16
Names
thyroid hormone receptor associated protein 5
thyroid hormone receptor-associated protein complex 95 kDa component
thyroid hormone receptor-associated protein, 95-kD subunit
vitamin D3 receptor-interacting protein complex 92 kDa component

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001163276.2 → NP_001156748.2  mediator of RNA polymerase II transcription subunit 16 isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) uses a different splice site in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter by 1 aa when it is compared to isoform 1.
    Source sequence(s)
    AC151846
    Consensus CDS
    CCDS48623.1
    UniProtKB/Swiss-Prot
    E9QM81, Q6PGF3
    Related
    ENSMUSP00000101017.4, ENSMUST00000105378.9

  2. NM_001359629.2 → NP_001346558.2  mediator of RNA polymerase II transcription subunit 16 isoform 3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The resulting protein (isoform 3), has a shorter and distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AC151846

  3. NM_001403459.1 → NP_001390388.1  mediator of RNA polymerase II transcription subunit 16 isoform 1

    Status: VALIDATED

    Source sequence(s)
    AC151846
    UniProtKB/Swiss-Prot
    E9QM81, Q6PGF3

  4. NM_198107.3 → NP_932775.3  mediator of RNA polymerase II transcription subunit 16 isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the longest protein (isoform 1).
    Source sequence(s)
    AC151846
    Consensus CDS
    CCDS23996.2
    UniProtKB/Swiss-Prot
    E9QM81, Q6PGF3
    Related
    ENSMUSP00000129375.3, ENSMUST00000165684.8

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000076.7 Reference GRCm39 C57BL/6J

    Range
    79730543..79744757 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036155735.1 → XP_036011628.1  mediator of RNA polymerase II transcription subunit 16 isoform X1

    UniProtKB/TrEMBL
    E9QP84
    Conserved Domains (3) summary
    smart00320
    Location:59 → 98
    WD40; WD40 repeats
    sd00039
    Location:26 → 68
    7WD40; WD40 repeat [structural motif]
    pfam11635
    Location:108 → 822
    Med16; Mediator complex subunit 16
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q6PGF3.2 GenPept UniProtKB/Swiss-Prot:Q6PGF3

Gene LinkOut

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