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Agap3 ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 [ Mus musculus (house mouse) ]

Gene ID: 213990, updated on 21-Apr-2024

Summary

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Official Symbol
Agap3provided by MGI
Official Full Name
ArfGAP with GTPase domain, ankyrin repeat and PH domain 3provided by MGI
Primary source
MGI:MGI:2183446
See related
Ensembl:ENSMUSG00000023353 AllianceGenome:MGI:2183446
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Crag; Crag.; AGAP-3; Centg3; MRIP-1; cnt-g3
Summary
Enables GTPase activity and polyubiquitin modification-dependent protein binding activity. Acts upstream of or within cellular response to reactive oxygen species; proteasome-mediated ubiquitin-dependent protein catabolic process; and protein import into nucleus. Located in cell periphery; cytoplasm; and nucleus. Orthologous to human AGAP3 (ArfGAP with GTPase domain, ankyrin repeat and PH domain 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in cerebellum adult (RPKM 21.6), cortex adult (RPKM 20.9) and 28 other tissues See more
Orthologs
human all
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Genomic context

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Location:
5 A3; 5 11.81 cM
Exon count:
20
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 5 NC_000071.7 (24657175..24707045)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 5 NC_000071.6 (24452177..24502047)

Chromosome 5 - NC_000071.7Genomic Context describing neighboring genes Neighboring gene Fas-activated serine/threonine kinase Neighboring gene transmembrane and ubiquitin-like domain containing 1 Neighboring gene VISTA enhancer mm1191 Neighboring gene gastrulation brain homeobox 1 Neighboring gene STARR-positive B cell enhancer mm9_chr5:24035915-24036216 Neighboring gene ankyrin repeat and SOCS box-containing 10

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Forebrain-specific deficiency of the GTPase CRAG/Centaurin-γ3 leads to immature dentate gyri and hyperactivity in mice. Nagashima S, et al. J Biol Chem, 2021 Jan-Jun. PMID 33811862, Free PMC Article
  2. Critical role of CRAG, a splicing variant of centaurin-γ3/AGAP3, in ELK1-dependent SRF activation at PML bodies. Nagashima S, et al. Sci Rep, 2019 Dec 27. PMID 31882856, Free PMC Article
  3. CRMP5-associated GTPase (CRAG) protein protects neuronal cells against cytotoxicity of expanded polyglutamine protein partially via c-Fos-dependent activator protein-1 activation. Nagashima S, et al. J Biol Chem, 2011 Sep 30. PMID 21832068, Free PMC Article
  4. A novel GTPase, CRAG, mediates promyelocytic leukemia protein-associated nuclear body formation and degradation of expanded polyglutamine protein. Qin Q, et al. J Cell Biol, 2006 Feb 13. PMID 16461359, Free PMC Article
  5. AGAP1, a novel binding partner of nitric oxide-sensitive guanylyl cyclase. Meurer S, et al. J Biol Chem, 2004 Nov 19. PMID 15381706

See all (26) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Novelty-induced memory consolidation is accompanied by increased Agap3 transcription: a cross-species study.
    Title: Novelty-induced memory consolidation is accompanied by increased Agap3 transcription: a cross-species study.
  2. Forebrain-specific deficiency of the GTPase CRAG/Centaurin-gamma3 leads to immature dentate gyri and hyperactivity in mice.
    Title: Forebrain-specific deficiency of the GTPase CRAG/Centaurin-γ3 leads to immature dentate gyri and hyperactivity in mice.
  3. CRAG enhances the cell survival signal against the accumulation of unfolded proteins, including polyQ, through not only proteasome activation, but also the activation of c-Fos-dependent AP-1.
    Title: CRMP5-associated GTPase (CRAG) protein protects neuronal cells against cytotoxicity of expanded polyglutamine protein partially via c-Fos-dependent activator protein-1 activation.
  4. We propose that CRAG is a modulator of promyelocytic leukemia protein function and dynamics in reactive oxygen species signaling and is protectively involved in the pathogenesis of polyglutamine diseases.
    Title: A novel GTPase, CRAG, mediates promyelocytic leukemia protein-associated nuclear body formation and degradation of expanded polyglutamine protein.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (2)  1 citation
  • Endonuclease-mediated (1) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC37541

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables GTP binding IEA
Inferred from Electronic Annotation
more info
  
enables GTPase activator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables GTPase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables GTPase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables polyubiquitin modification-dependent protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within PML body organization ISO
Inferred from Sequence Orthology
more info
 PubMed 
involved_in activation of GTPase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within cellular response to UV ISO
Inferred from Sequence Orthology
more info
 PubMed 
acts_upstream_of_or_within cellular response to reactive oxygen species IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within cellular response to reactive oxygen species ISO
Inferred from Sequence Orthology
more info
 PubMed 
acts_upstream_of_or_within proteasome-mediated ubiquitin-dependent protein catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within protein import into nucleus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in PML body ISO
Inferred from Sequence Orthology
more info
 PubMed 
located_in cell periphery IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3
Names
CRAM-associated GTPase
MR1-interacting protein
centaurin, gamma 3
spidin

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001256431.1 → NP_001243360.1  arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform b

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) lacks several exons and includes an alternate 3' terminal exon, compared to variant 1. It encodes isoform b which is shorter and has a distinct C-terminus, compared to isoform a.
    Source sequence(s)
    AC120353
    UniProtKB/TrEMBL
    A0A1D5RMG4
    Related
    ENSMUSP00000148770.2, ENSMUST00000212381.2
    Conserved Domains (2) summary
    cd04103
    Location:127 → 284
    Centaurin_gamma; Centaurin gamma (CENTG) GTPase
    smart00010
    Location:125 → 290
    small_GTPase; Small GTPase of the Ras superfamily; ill-defined subfamily

  2. NM_001359881.1 → NP_001346810.1  arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform 3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) lacks two alternate in-frame exons compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1.
    Source sequence(s)
    AC120353
    UniProtKB/Swiss-Prot
    Q812F7, Q8VHH5
    Conserved Domains (5) summary
    cd04103
    Location:127 → 284
    Centaurin_gamma; Centaurin gamma (CENTG) GTPase
    cd00204
    Location:685 → 774
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    cd01250
    Location:400 → 541
    PH_AGAP; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein Pleckstrin homology (PH) domain
    sd00045
    Location:685 → 715
    ANK; ANK repeat [structural motif]
    pfam01412
    Location:560 → 674
    ArfGap; Putative GTPase activating protein for Arf

  3. NM_139153.2 → NP_631892.2  arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform a

    See identical proteins and their annotated locations for NP_631892.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).
    Source sequence(s)
    AC120353, AK079516, AV241066, BM943676, BM943884, CJ052480, CJ053222
    Consensus CDS
    CCDS19122.1
    UniProtKB/Swiss-Prot
    Q812F7, Q8VHH5
    UniProtKB/TrEMBL
    F8VQE9
    Related
    ENSMUSP00000024123.5, ENSMUST00000024123.9
    Conserved Domains (6) summary
    cd04103
    Location:127 → 284
    Centaurin_gamma; Centaurin gamma (CENTG) GTPase
    PRK07003
    Location:462 → 573
    PRK07003; DNA polymerase III subunit gamma/tau
    cd01250
    Location:400 → 644
    PH_AGAP; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein Pleckstrin homology (PH) domain
    sd00045
    Location:788 → 818
    ANK; ANK repeat [structural motif]
    cd08855
    Location:660 → 769
    ArfGap_AGAP3; ArfGAP with GTPase domain, ANK repeat and PH domain 3
    pfam12796
    Location:788 → 878
    Ank_2; Ankyrin repeats (3 copies)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000071.7 Reference GRCm39 C57BL/6J

    Range
    24657175..24707045
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006535676.5 → XP_006535739.1  arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform X4

    UniProtKB/TrEMBL
    A0A0G2JDW1
    Related
    ENSMUSP00000142529.2, ENSMUST00000199856.2
    Conserved Domains (1) summary
    cd04103
    Location:310 → 467
    Centaurin_gamma; Centaurin gamma (CENTG) GTPase

  2. XM_006535673.5 → XP_006535736.1  arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform X1

    Conserved Domains (6) summary
    cd04103
    Location:310 → 467
    Centaurin_gamma; Centaurin gamma (CENTG) GTPase
    PRK07003
    Location:645 → 756
    PRK07003; DNA polymerase III subunit gamma/tau
    cd01250
    Location:583 → 827
    PH_AGAP; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein Pleckstrin homology (PH) domain
    sd00045
    Location:971 → 1001
    ANK; ANK repeat [structural motif]
    cd08855
    Location:843 → 952
    ArfGap_AGAP3; ArfGAP with GTPase domain, ANK repeat and PH domain 3
    pfam12796
    Location:971 → 1061
    Ank_2; Ankyrin repeats (3 copies)

  3. XM_006535674.5 → XP_006535737.1  arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform X2

    Conserved Domains (5) summary
    cd04103
    Location:310 → 467
    Centaurin_gamma; Centaurin gamma (CENTG) GTPase
    cd01250
    Location:583 → 781
    PH_AGAP; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein Pleckstrin homology (PH) domain
    sd00045
    Location:925 → 955
    ANK; ANK repeat [structural motif]
    cd08855
    Location:797 → 906
    ArfGap_AGAP3; ArfGAP with GTPase domain, ANK repeat and PH domain 3
    pfam12796
    Location:925 → 1015
    Ank_2; Ankyrin repeats (3 copies)

  4. XM_006535675.5 → XP_006535738.1  arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform X3

    Conserved Domains (5) summary
    cd04103
    Location:310 → 467
    Centaurin_gamma; Centaurin gamma (CENTG) GTPase
    cd01250
    Location:583 → 724
    PH_AGAP; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein Pleckstrin homology (PH) domain
    sd00045
    Location:868 → 898
    ANK; ANK repeat [structural motif]
    cd08855
    Location:740 → 849
    ArfGap_AGAP3; ArfGAP with GTPase domain, ANK repeat and PH domain 3
    pfam12796
    Location:868 → 958
    Ank_2; Ankyrin repeats (3 copies)

  5. XM_006535677.4 → XP_006535740.1  arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform X5

    Conserved Domains (5) summary
    PRK07003
    Location:117 → 228
    PRK07003; DNA polymerase III subunit gamma/tau
    cd01250
    Location:55 → 299
    PH_AGAP; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein Pleckstrin homology (PH) domain
    sd00045
    Location:443 → 473
    ANK; ANK repeat [structural motif]
    cd08855
    Location:315 → 424
    ArfGap_AGAP3; ArfGAP with GTPase domain, ANK repeat and PH domain 3
    pfam12796
    Location:443 → 533
    Ank_2; Ankyrin repeats (3 copies)

  6. XM_030254355.1 → XP_030110215.1  arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform X6

    UniProtKB/TrEMBL
    A0A0G2JER6
    Related
    ENSMUSP00000142875.2, ENSMUST00000197513.2
    Conserved Domains (4) summary
    cd01250
    Location:55 → 196
    PH_AGAP; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein Pleckstrin homology (PH) domain
    sd00045
    Location:340 → 370
    ANK; ANK repeat [structural motif]
    cd08855
    Location:212 → 321
    ArfGap_AGAP3; ArfGAP with GTPase domain, ANK repeat and PH domain 3
    pfam12796
    Location:340 → 430
    Ank_2; Ankyrin repeats (3 copies)

RNA

  1. XR_001784662.3 RNA Sequence

  2. XR_001784661.3 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8VHH5.1 GenPept UniProtKB/Swiss-Prot:Q8VHH5

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