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Pm20d1 peptidase M20 domain containing 1 [ Mus musculus (house mouse) ]

Gene ID: 212933, updated on 21-Apr-2024

Summary

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Official Symbol
Pm20d1provided by MGI
Official Full Name
peptidase M20 domain containing 1provided by MGI
Primary source
MGI:MGI:2442939
See related
Ensembl:ENSMUSG00000042251 AllianceGenome:MGI:2442939
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
4732466D17Rik
Summary
Enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides. Involved in several processes, including cellular amide catabolic process; energy homeostasis; and regulation of oxidative phosphorylation uncoupler activity. Located in extracellular space. Is expressed in embryo; embryo endoderm; and notochord. Orthologous to human PM20D1 (peptidase M20 domain containing 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in liver adult (RPKM 26.9), large intestine adult (RPKM 13.8) and 10 other tissues See more
Orthologs
human all
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Genomic context

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Location:
1 E4; 1 57.19 cM
Exon count:
15
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (131725122..131749210)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (131797385..131821472)

Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 30104 Neighboring gene solute carrier family 26, member 9 Neighboring gene STARR-seq mESC enhancer starr_02224 Neighboring gene STARR-seq mESC enhancer starr_02225 Neighboring gene STARR-positive B cell enhancer ABC_E8960 Neighboring gene solute carrier family 41, member 1 Neighboring gene STARR-positive B cell enhancer ABC_E5799 Neighboring gene RAB29, member RAS oncogene family

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Ablation of PM20D1 reveals N-acyl amino acid control of metabolism and nociception. Long JZ, et al. Proc Natl Acad Sci U S A, 2018 Jul 17. PMID 29967167, Free PMC Article
  2. Kir6.2 is essential to maintain neurite features by modulating PM20D1-reduced mitochondrial ATP generation. Song N, et al. Redox Biol, 2021 Nov. PMID 34673451, Free PMC Article
  3. Inhibition of miR-324-5p increases PM20D1-mediated white and brown adipose loss and reduces body weight in juvenile mice. Li D, et al. Eur J Pharmacol, 2019 Nov 15. PMID 31568785
  4. The Secreted Enzyme PM20D1 Regulates Lipidated Amino Acid Uncouplers of Mitochondria. Long JZ, et al. Cell, 2016 Jul 14. PMID 27374330, Free PMC Article
  5. The Metabolic Reprogramming of Frem2 Mutant Mice Embryos in Cryptophthalmos Development. Zhang X, et al. Front Cell Dev Biol, 2020. PMID 33490088, Free PMC Article

See all (16) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Kir6.2 is essential to maintain neurite features by modulating PM20D1-reduced mitochondrial ATP generation.
    Title: Kir6.2 is essential to maintain neurite features by modulating PM20D1-reduced mitochondrial ATP generation.
  2. Inhibition of miR-324-5p increases PM20D1-mediated white and brown adipose loss and reduces body weight in juvenile mice
    Title: Inhibition of miR-324-5p increases PM20D1-mediated white and brown adipose loss and reduces body weight in juvenile mice.
  3. These data demonstrate that PM20D1 is a dominant enzymatic regulator of NAA levels in vivo and elucidate physiologic functions for NAA signaling in metabolism and nociception.
    Title: Ablation of PM20D1 reveals N-acyl amino acid control of metabolism and nociception.
  4. Study demonstrates that PM20D1 is a bidirectional enzyme in vitro, catalyzing both the condensation of fatty acids and amino acids to generate N-acyl amino acids and also the reverse hydrolytic reaction. N-acyl amino acids directly bind mitochondria and function as endogenous uncouplers of UCP1-independent respiration.
    Title: The Secreted Enzyme PM20D1 Regulates Lipidated Amino Acid Uncouplers of Mitochondria.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (1) 
  • Endonuclease-mediated (5)  1 citation

Pathways from PubChem

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General gene information

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Markers

Homology

Clone Names

  • MGC159098

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables aminoacylase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides IBA
Inferred from Biological aspect of Ancestor
more info
  
enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides IDA
Inferred from Direct Assay
more info
 PubMed 
enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides ISO
Inferred from Sequence Orthology
more info
  
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables peptidase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in adaptive thermogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in amide biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in amide biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in amide biosynthetic process ISO
Inferred from Sequence Orthology
more info
  
involved_in amide catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in amide catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in amide catabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in amino acid metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in amino acid metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in amino acid metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in cellular lipid metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in cellular lipid metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cellular lipid metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in energy homeostasis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within lipid metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in nitrogen compound metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within proteolysis IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in extracellular region IEA
Inferred from Electronic Annotation
more info
  
located_in extracellular space IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
N-fatty-acyl-amino acid synthase/hydrolase PM20D1
Names
peptidase M20 domain-containing protein 1
probable carboxypeptidase PM20D1
NP_001344407.1
NP_835180.2
XP_006529402.1
XP_011246269.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001357478.1 → NP_001344407.1  N-fatty-acyl-amino acid synthase/hydrolase PM20D1 precursor

    Status: VALIDATED

    Source sequence(s)
    AC161805, AK028883
    Consensus CDS
    CCDS15274.1
    UniProtKB/Swiss-Prot
    Q3UNX0, Q8C165, Q8R117
    Related
    ENSMUSP00000046079.6, ENSMUST00000048660.12
    Conserved Domains (1) summary
    cd05674
    Location:53 → 488
    M20_yscS; M20 Peptidase, carboxypeptidase yscS

  2. NM_178079.3 → NP_835180.2  N-fatty-acyl-amino acid synthase/hydrolase PM20D1 precursor

    See identical proteins and their annotated locations for NP_835180.2

    Status: VALIDATED

    Source sequence(s)
    AC161805
    Consensus CDS
    CCDS15274.1
    UniProtKB/Swiss-Prot
    Q3UNX0, Q8C165, Q8R117
    Related
    ENSMUSP00000108012.3, ENSMUST00000112393.9
    Conserved Domains (1) summary
    cd05674
    Location:53 → 488
    M20_yscS; M20 Peptidase, carboxypeptidase yscS

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000067.7 Reference GRCm39 C57BL/6J

    Range
    131725122..131749210
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011247967.4 → XP_011246269.1  N-fatty-acyl-amino acid synthase/hydrolase PM20D1 isoform X1

    Conserved Domains (1) summary
    cd05674
    Location:53 → 463
    M20_yscS; M20 Peptidase, carboxypeptidase yscS

  2. XM_006529339.2 → XP_006529402.1  N-fatty-acyl-amino acid synthase/hydrolase PM20D1 isoform X2

    Conserved Domains (1) summary
    cd05674
    Location:53 → 463
    M20_yscS; M20 Peptidase, carboxypeptidase yscS
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8C165.1 GenPept UniProtKB/Swiss-Prot:Q8C165

Gene LinkOut

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