Slc1a3 solute carrier family 1 (glial high affinity glutamate transporter), member 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Slc1a3provided by MGI
Official Full Name: solute carrier family 1 (glial high affinity glutamate transporter), member 3provided by MGI
Primary source: MGI:MGI:99917
See related: Ensembl:ENSMUSG00000005360 AllianceGenome:MGI:99917
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Gmt1; Eaat1; GLAST; GLU-T; GluT-1; MGluT1; GLAST-1; B430115D02Rik
Summary: Enables glutamate binding activity and high-affinity glutamate transmembrane transporter activity. Involved in D-aspartate import across plasma membrane and cellular response to cocaine. Acts upstream of or within several processes, including L-glutamate import across plasma membrane; gamma-aminobutyric acid biosynthetic process; and nervous system development. Located in several cellular components, including cell surface; neuron projection; and neuronal cell body. Is integral component of plasma membrane. Is expressed in several structures, including brain; connective tissue; eye; genitourinary system; and spinal cord. Used to study low tension glaucoma. Human ortholog(s) of this gene implicated in episodic ataxia type 6. Orthologous to human SLC1A3 (solute carrier family 1 member 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in frontal lobe adult (RPKM 107.1), cerebellum adult (RPKM 104.8) and 8 other tissues See more
Orthologs: human all
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Genomic context

Location:: 15 A1; 15 3.82 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (8663608..8742648, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (8634124..8710807, complement)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

[Effects of GLAST gene knockout on phenotype and hearing in mice].
Title: [Effects of GLAST gene knockout on phenotype and hearing in mice].
Early postnatal inhibition of GLAST causes abnormalities of psychobehaviors and neuronal morphology in adult mice.
Title: Early postnatal inhibition of GLAST causes abnormalities of psychobehaviors and neuronal morphology in adult mice.
Chronic optogenetic stimulation of Bergman glia leads to dysfunction of EAAT1 and Purkinje cell death, mimicking the events caused by expression of pathogenic ataxin-1.
Title: Chronic optogenetic stimulation of Bergman glia leads to dysfunction of EAAT1 and Purkinje cell death, mimicking the events caused by expression of pathogenic ataxin-1.
Auditory synaptopathy in mice lacking the glutamate transporter GLAST and its impact on brain activity.
Title: Auditory synaptopathy in mice lacking the glutamate transporter GLAST and its impact on brain activity.
Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background.
Title: Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background.
Circadian vulnerability of cisplatin-induced ototoxicity in the cochlea.
Title: Circadian vulnerability of cisplatin-induced ototoxicity in the cochlea.
Defining compartmentalized stem cell populations with distinct cell division dynamics in the ocular surface epithelium.
Title: Defining compartmentalized stem cell populations with distinct cell division dynamics in the ocular surface epithelium.
In vivo knockdown of astroglial glutamate transporters GLT-1 and GLAST increases excitatory neurotransmission in mouse infralimbic cortex: Relevance for depressive-like phenotypes.
Title: In vivo knockdown of astroglial glutamate transporters GLT-1 and GLAST increases excitatory neurotransmission in mouse infralimbic cortex: Relevance for depressive-like phenotypes.
In the present study, we found for the first time the dilation of the retinal blood vessels and the thinning of the outer nuclear layer in GLAST(-/-) mice, in addition to the thinning of the ganglion cell complex (GCC).
Title: Excess Glutamate May Cause Dilation of Retinal Blood Vessels in Glutamate/Aspartate Transporter-Deficient Mice.
SLC1A3 rs1049522 showed significant association with ADHD risk. rs1049522-C allele enhanced SLC1A3 expression by disrupting the hsa-miR-3171 binding site.
Title: A functional variant in SLC1A3 influences ADHD risk by disrupting a hsa-miR-3171 binding site: A two-stage association study.

Submit: New GeneRIF Correction See all GeneRIFs (58)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (8) 1 citation
Endonuclease-mediated (8) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Slc1a3 (e-PCR), detects polymorphism
- UniSTS:144417

Slc1a2 (e-PCR), detects polymorphism
- UniSTS:465496

PMC311006P7 (e-PCR)
- UniSTS:272834

Slc1a3 (e-PCR), detects polymorphism
- UniSTS:547015

AI504299 (e-PCR)
- UniSTS:180734

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables L-glutamate transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables L-glutamate transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables acidic amino acid transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables amino acid binding	IDA Inferred from Direct Assay more info	PubMed
enables glutamate binding	IDA Inferred from Direct Assay more info	PubMed
enables glutamate:sodium symporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables glutamate:sodium symporter activity	ISO Inferred from Sequence Orthology more info
enables high-affinity L-glutamate transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables high-affinity L-glutamate transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables high-affinity L-glutamate transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables neutral L-amino acid transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables symporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in D-aspartate import across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in D-aspartate import across plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in D-aspartate import across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in L-aspartate import across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in L-aspartate import across plasma membrane	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within L-glutamate import	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in L-glutamate import	ISO Inferred from Sequence Orthology more info
involved_in L-glutamate import across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within L-glutamate import across plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in L-glutamate import across plasma membrane	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within L-glutamate transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within L-glutamate transmembrane transport	ISO Inferred from Sequence Orthology more info	PubMed
involved_in L-glutamate transmembrane transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within amino acid transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within auditory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell morphogenesis involved in neuron differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to cocaine	IDA Inferred from Direct Assay more info	PubMed
involved_in chloride transmembrane transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cranial nerve development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within gamma-aminobutyric acid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within intracellular sodium ion homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in malate-aspartate shuttle	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neuromuscular process controlling balance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in potassium ion transmembrane transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to amino acid	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within response to antibiotic	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to inorganic substance	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within response to light stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to wounding	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to xenobiotic stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within sensory perception of sound	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in basal plasma membrane	ISO Inferred from Sequence Orthology more info
located_in basolateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in cell periphery	IDA Inferred from Direct Assay more info	PubMed
located_in cell projection	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic spine	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
part_of membrane protein complex	ISO Inferred from Sequence Orthology more info
located_in mitochondrial inner membrane	ISO Inferred from Sequence Orthology more info
located_in neuron projection	IDA Inferred from Direct Assay more info	PubMed
located_in neuron projection	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	TAS Traceable Author Statement more info	PubMed
located_in synapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: excitatory amino acid transporter 1

Names: glial high affinity glutamate transporter; glutamate/aspartate transporter; high-affinity neuronal glutamate transporter; sodium-dependent glutamate/aspartate transporter 1; solute carrier family 1, member 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_148938.3 → NP_683740.1 excitatory amino acid transporter 1

See identical proteins and their annotated locations for NP_683740.1

Status: VALIDATED

Source sequence(s)

AK031831, AK040798, BC058711, BY281420

Consensus CDS

CCDS27373.1

UniProtKB/Swiss-Prot

P56564, Q99P53

UniProtKB/TrEMBL

Q543U3, Q8C3T7, Q8C7W8

Related

ENSMUSP00000005493.8, ENSMUST00000005493.14

Conserved Domains (1) summary

pfam00375
Location:50 → 497

SDF; Sodium:dicarboxylate symporter family

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000081.7 Reference GRCm39 C57BL/6J

Range

8663608..8742648 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036159289.1 → XP_036015182.1 excitatory amino acid transporter 1 isoform X1

UniProtKB/TrEMBL

Q8C3T7, Q8C7W8

Conserved Domains (1) summary

pfam00375
Location:5 → 390

SDF; Sodium:dicarboxylate symporter family