St8sia2 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: St8sia2provided by MGI
Official Full Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2provided by MGI
Primary source: MGI:MGI:106020
See related: Ensembl:ENSMUSG00000025789 AllianceGenome:MGI:106020
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: STX; Siat8b; ST8SiaII
Summary: Enables alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity. Predicted to be involved in several processes, including ganglioside biosynthetic process; glycoprotein metabolic process; and neuron projection extension. Located in early endosome and recycling endosome. Is expressed in several structures, including central nervous system; future brain; genitourinary system; gut; and retina. Orthologous to human ST8SIA2 (ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in whole brain E14.5 (RPKM 29.1), CNS E14 (RPKM 24.1) and 5 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 D1; 7 42.27 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (73588867..73663408, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (73939119..74013727, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Compromised mammillary body connectivity and psychotic symptoms in mice with di- and mesencephalic ablation of ST8SIA2.
Title: Compromised mammillary body connectivity and psychotic symptoms in mice with di- and mesencephalic ablation of ST8SIA2.
Amygdala GluN2B-NMDAR dysfunction is critical in abnormal aggression of neurodevelopmental origin induced by St8sia2 deficiency.
Title: Amygdala GluN2B-NMDAR dysfunction is critical in abnormal aggression of neurodevelopmental origin induced by St8sia2 deficiency.
Effect of expression alteration in flanking genes on phenotypes of St8sia2-deficient mice.
Title: Effect of expression alteration in flanking genes on phenotypes of St8sia2-deficient mice.
the data demonstrate a cell-autonomous impact of ST8SIA2 on cortical interneuron migration and the distribution of parvalbumin-positive interneurons in the anterior cortex
Title: Cell-autonomous impact of polysialic acid-producing enzyme ST8SIA2 on developmental migration and distribution of cortical interneurons.
Results demonstrate that the early development of calbindin-positive periglomerular interneurons depends on the presentation of polysialic acid on NCAM and requires the activity of both St8sia2 and St8sia4
Title: Deficits of olfactory interneurons in polysialyltransferase- and NCAM-deficient mice.
NCAM-140 significantly promoted cell proliferation, motility and migration, while polysialylation of NCAM-140 catalyzed by STX enhanced NCAM-mediated cell migration, but not cell proliferation or motility.
Title: Promotion of cell migration by neural cell adhesion molecule (NCAM) is enhanced by PSA in a polysialyltransferase-specific manner.
These results reveal that reduced polysialylation in St8sia2 (-/-) mice leads to pathological brain development and schizophrenia-like behavior.
Title: Schizophrenia-like phenotype of polysialyltransferase ST8SIA2-deficient mice.
This study demonstrated that St8sia2 deficiency plus juvenile cannabis exposure in mice synergistically affect higher cognition in adulthood.
Title: St8sia2 deficiency plus juvenile cannabis exposure in mice synergistically affect higher cognition in adulthood.
Results indicate that the polySTs ST8SiaII and ST8SiaIV not only recognize an acidic patch in the FN1 domain of NCAM but also must contact sequences in the Ig5 domain for polysialylation of Ig5 N-glycans to occur.
Title: The polysialyltransferases interact with sequences in two domains of the neural cell adhesion molecule to allow its polysialylation.
Polysialylation of the synaptic cell adhesion molecule 1 (SynCAM 1) depends exclusively on the polysialyltransferase ST8SiaII in vivo.
Title: Polysialylation of the synaptic cell adhesion molecule 1 (SynCAM 1) depends exclusively on the polysialyltransferase ST8SiaII in vivo.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Siat8b (e-PCR), detects polymorphism
- UniSTS:261921

STS-L29556 (e-PCR)
- UniSTS:73508

AI323367 (e-PCR)
- UniSTS:179588

St8sia2 (e-PCR), detects polymorphism
- UniSTS:496777

D7Dcr30 (e-PCR), detects polymorphism
- UniSTS:507788

D7Mit318 (e-PCR), detects polymorphism
- UniSTS:131219

D1Bda26 (e-PCR)
- UniSTS:239773

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity	ISO Inferred from Sequence Orthology more info
enables glycosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sialyltransferase activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in N-glycan processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in N-glycan processing	ISO Inferred from Sequence Orthology more info
involved_in ganglioside biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in neuron projection extension	ISO Inferred from Sequence Orthology more info
involved_in oligosaccharide metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in oligosaccharide metabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neuron apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of synapse assembly	ISO Inferred from Sequence Orthology more info
involved_in protein glycosylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein glycosylation	ISO Inferred from Sequence Orthology more info
involved_in sialylation	IDA Inferred from Direct Assay more info	PubMed
involved_in sialylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sialylation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in early endosome	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in recycling endosome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: alpha-2,8-sialyltransferase 8B

Names: SIAT8-B; polysialic acid synthase; sialyltransferase 8 (alpha-2, 8-sialyltransferase) B; sialyltransferase 8B; sialyltransferase St8Sia II; sialyltransferase X; sialytransferase St8Sia II

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001417262.1 → NP_001404191.1 alpha-2,8-sialyltransferase 8B isoform 2 precursor

Status: VALIDATED

Source sequence(s)

AC101658, AC162361
NM_009181.3 → NP_033207.2 alpha-2,8-sialyltransferase 8B isoform 1 precursor

See identical proteins and their annotated locations for NP_033207.2

Status: VALIDATED

Source sequence(s)

AC101658, AC162361

Consensus CDS

CCDS21365.1

UniProtKB/Swiss-Prot

O35696, Q4VA47

Related

ENSMUSP00000026896.5, ENSMUST00000026896.10

Conserved Domains (1) summary

pfam00777
Location:109 → 368

Glyco_transf_29; Glycosyltransferase family 29 (sialyltransferase)