Twf1 twinfilin actin binding protein 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Twf1provided by MGI
Official Full Name: twinfilin actin binding protein 1provided by MGI
Primary source: MGI:MGI:1100520
See related: Ensembl:ENSMUSG00000022451 AllianceGenome:MGI:1100520
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: A6; Ptk9; twinfilin
Summary: Enables actin monomer binding activity; phosphatidylinositol-4,5-bisphosphate binding activity; and protein tyrosine kinase activity. Involved in barbed-end actin filament capping; positive regulation of cardiac muscle hypertrophy; and sequestering of actin monomers. Located in several cellular components, including filopodium; myofibril; and perinuclear region of cytoplasm. Colocalizes with ruffle membrane. Is expressed in several structures, including alimentary system; genitourinary system; nervous system; respiratory system; and sensory organ. Orthologous to human TWF1 (twinfilin actin binding protein 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in bladder adult (RPKM 32.3), large intestine adult (RPKM 27.6) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 15 E3; 15 48.59 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (94475829..94487727, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (94577948..94589884, complement)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Twinfilin-1 is an essential regulator of myogenic differentiation through the modulation of YAP in C2C12 myoblasts.
Title: Twinfilin-1 is an essential regulator of myogenic differentiation through the modulation of YAP in C2C12 myoblasts.
Structural Insights into the Regulation of Actin Capping Protein by Twinfilin C-terminal Tail.
Title: Structural Insights into the Regulation of Actin Capping Protein by Twinfilin C-terminal Tail.
Actin/microtubule crosstalk during platelet biogenesis in mice is critically regulated by Twinfilin1 and Cofilin1.
Title: Actin/microtubule crosstalk during platelet biogenesis in mice is critically regulated by Twinfilin1 and Cofilin1.
Twinfilin bypasses assembly conditions and actin filament aging to drive barbed end depolymerization.
Title: Twinfilin bypasses assembly conditions and actin filament aging to drive barbed end depolymerization.
Twinfilin uncaps filament barbed ends to promote turnover of lamellipodial actin networks.
Title: Twinfilin uncaps filament barbed ends to promote turnover of lamellipodial actin networks.
The results demonstrate that the conserved C-terminal tail of twinfilin is a multifunctional binding motif, which is crucial for interaction with the heterodimeric capping protein and for tethering twinfilin to phosphoinositide-rich membranes.
Title: Molecular mechanism for inhibition of twinfilin by phosphoinositides.
Here, the authors discover that the C-terminal tail of Twinfilin harbors a capping protein-interacting (CPI) motif, identifying it as a novel CPI-motif protein. Twinfilin binds competitively with CARMIL to capping protein, protecting capping protein from barbed-end displacement by CARMIL.
Title: A novel mode of capping protein-regulation by twinfilin.
FGF signaling promotes the expansion of A6-expressing liver cells partly via AKT-dependent activation of beta-Catenin expansion of A6(+ive) periportal cells and possibly by reprogramming of centrolobular hepatocytes.
Title: Fibroblast growth factor signaling regulates the expansion of A6-expressing hepatocytes in association with AKT-dependent β-catenin activation.
like Twf1, mouse Twf2 is a filament barbed-end capping protein, and that two tissue-specific and biochemically distinct isoforms are generated from the Twf2 gene through alternative promoter usage
Title: Two biochemically distinct and tissue-specific twinfilin isoforms are generated from the mouse Twf2 gene by alternative promoter usage.
The crystal structure of twinfilin's C-terminal ADF-H domain in complex with an actin monomer, is presented.
Title: Structure of the actin-depolymerizing factor homology domain in complex with actin.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1)
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:237382

RH143291 (e-PCR)
- UniSTS:232538

Twf1 (e-PCR), detects polymorphism
- UniSTS:507295

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables actin binding	ISO Inferred from Sequence Orthology more info
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin monomer binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin monomer binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-4,5-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein tyrosine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in actin filament depolymerization	IBA Inferred from Biological aspect of Ancestor more info
involved_in barbed-end actin filament capping	IBA Inferred from Biological aspect of Ancestor more info
involved_in barbed-end actin filament capping	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of actin filament polymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of cardiac muscle hypertrophy	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of neuron projection development	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of actin phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of lamellipodium assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in sequestering of actin monomers	IBA Inferred from Biological aspect of Ancestor more info
involved_in sequestering of actin monomers	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
is_active_in actin filament	IBA Inferred from Biological aspect of Ancestor more info
located_in cell-cell junction	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
located_in filopodium	IDA Inferred from Direct Assay more info	PubMed
is_active_in myofibril	IBA Inferred from Biological aspect of Ancestor more info
located_in myofibril	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with ruffle membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: twinfilin-1

Names: PTK9 protein tyrosine kinase 9; actin monomer-binding protein; protein A6; protein tyrosine kinase 9; twinfilin, actin-binding protein, homolog 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001402809.1 → NP_001389738.1 twinfilin-1 isoform 2

Status: VALIDATED

Source sequence(s)

AC147160
NM_001402810.1 → NP_001389739.1 twinfilin-1 isoform 3

Status: VALIDATED

Source sequence(s)

AC147160
NM_008971.5 → NP_032997.3 twinfilin-1 isoform 1

See identical proteins and their annotated locations for NP_032997.3

Status: VALIDATED

Source sequence(s)

AC147160

Consensus CDS

CCDS27773.1

UniProtKB/Swiss-Prot

O09132, Q52L77, Q80X09, Q91YR1

Related

ENSMUSP00000023087.7, ENSMUST00000023087.13

Conserved Domains (2) summary

cd11284
Location:176 → 311

ADF_Twf-C_like; C-terminal ADF domain of twinfilin and related proteins

cd11285
Location:4 → 142

ADF_Twf-N_like; N-terminal ADF domain of twinfilin and related proteins