Pik3r2 phosphoinositide-3-kinase regulatory subunit 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pik3r2provided by MGI
Official Full Name: phosphoinositide-3-kinase regulatory subunit 2provided by MGI
Primary source: MGI:MGI:1098772
See related: Ensembl:ENSMUSG00000031834 AllianceGenome:MGI:1098772
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: p85beta
Summary: Enables phosphatidylinositol 3-kinase regulatory subunit binding activity and protein heterodimerization activity. Involved in several processes, including cellular glucose homeostasis; insulin receptor signaling pathway; and positive regulation of protein import into nucleus. Acts upstream of or within positive regulation of cell adhesion; regulation of actin filament organization; and regulation of protein localization to plasma membrane. Located in focal adhesion and nucleus. Is expressed in several structures, including early embryo; genitourinary system; liver; musculature; and spleen. Human ortholog(s) of this gene implicated in pancreatic ductal adenocarcinoma. Orthologous to human PIK3R2 (phosphoinositide-3-kinase regulatory subunit 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in ovary adult (RPKM 65.5), duodenum adult (RPKM 57.1) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 B3.3; 8 34.15 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (71220820..71229356, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (70768176..70776712, complement)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Anthrax lethal factor cleaves regulatory subunits of phosphoinositide-3 kinase to contribute to toxin lethality.
Title: Anthrax lethal factor cleaves regulatory subunits of phosphoinositide-3 kinase to contribute to toxin lethality.
p85beta deficiency does not alter NK cell differentiation and maturation in spleen or bone marrow. NK cells from p85beta(-/-) mice nonetheless produced more IFN-gamma and degranulated more effectively when stimulated with anti-NKG2D antibody. p85beta deficiency impaired NKG2D internalization, which could contribute to the activated phenotype.
Title: PI3K p85 β regulatory subunit deficiency does not affect NK cell differentiation and increases NKG2D-mediated activation.
Activation of PI3K (p110beta and p85beta), and phospho-AKT (Ser473), participated in the regulation of activating macrophages by wear particles, ultimately resulting in the secretion of TNF-alpha.
Title: Inhibition of the PI3K/AKT pathway reduces tumor necrosis factor-alpha production in the cellular response to wear particles in vitro.
miR-126-mediated phosphoinositide-3-kinase regulation, not only fine-tunes VEGF-signaling, but it strongly enhances the activities of Ang-1 on vessel stabilization and maturation.
Title: The miR-126 regulates angiopoietin-1 signaling and vessel maturation by targeting p85β.
p85beta phosphoinositide 3-kinase subunit regulates tumor progression
Title: p85β phosphoinositide 3-kinase subunit regulates tumor progression.
study shows that p110beta nuclear localization signal and p85beta nuclear export sequence regulate p85beta/p110beta nuclear localization, supporting the idea that nuclear, but not cytoplasmic, p110beta controls cell survival
Title: Nuclear but not cytosolic phosphoinositide 3-kinase beta has an essential function in cell survival.
heterodimerization disrupted by insulin, increasing nuclear translocation of XBP-1
Title: The regulatory subunits of PI3K, p85alpha and p85beta, interact with XBP-1 and increase its nuclear translocation.
These results indicate that the p85beta regulatory isoform has partially overlapping functions with p85alpha in B cells as well as a unique role in opposing BCR responses.
Title: The p85beta regulatory subunit of phosphoinositide 3-kinase has unique and redundant functions in B cells.
Data show that mesothelin is a potential target in reducing resistance to cytotoxic drugs, and mesothelin-treated cells revealed rapid tyrosine phosphorylation of the p85 subunit of PI3K.
Title: Mesothelin inhibits paclitaxel-induced apoptosis through the PI3K pathway.
Involvement of p85beta in CD28-mediated activation and differentiation of antigen-stimulated T cells.
Title: p85beta phosphoinositide 3-kinase regulates CD28 coreceptor function.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 1-phosphatidylinositol-3-kinase regulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylinositol 3-kinase regulatory subunit binding	IPI Inferred from Physical Interaction more info	PubMed
enables phosphotyrosine residue binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein heterodimerization activity	IPI Inferred from Physical Interaction more info	PubMed
enables protein phosphatase binding	ISO Inferred from Sequence Orthology more info
enables receptor tyrosine kinase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cellular response to insulin stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in insulin receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in insulin receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular glucose homeostasis	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of MAPK cascade	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell migration involved in sprouting angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylinositol 3-kinase/protein kinase B signal transduction	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylinositol phosphate biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of cell adhesion	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein import into nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of actin filament polymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of autophagy	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of protein localization to plasma membrane	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of stress fiber assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to endoplasmic reticulum stress	IDA Inferred from Direct Assay more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in focal adhesion	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of phosphatidylinositol 3-kinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of phosphatidylinositol 3-kinase complex, class IA	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: phosphatidylinositol 3-kinase regulatory subunit beta

Names: PI3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta; PI3K regulatory subunit beta; phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta); ptdIns-3-kinase regulatory subunit p85-beta

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001424805.1 → NP_001411734.1 phosphatidylinositol 3-kinase regulatory subunit beta isoform 2

Status: VALIDATED

Source sequence(s)

AC162446
NM_001424806.1 → NP_001411735.1 phosphatidylinositol 3-kinase regulatory subunit beta isoform 3

Status: VALIDATED

Source sequence(s)

AC162446
NM_008841.3 → NP_032867.2 phosphatidylinositol 3-kinase regulatory subunit beta isoform 1

See identical proteins and their annotated locations for NP_032867.2

Status: VALIDATED

Source sequence(s)

AC162446

Consensus CDS

CCDS22381.1

UniProtKB/Swiss-Prot

O08908, Q5U3K7

UniProtKB/TrEMBL

A0A2X0SZ21

Related

ENSMUSP00000034296.9, ENSMUST00000034296.15

Conserved Domains (6) summary

cd09930
Location:609 → 712

SH2_cSH2_p85_like; C-terminal Src homology 2 (cSH2) domain found in p85

cd09942
Location:316 → 426

SH2_nSH2_p85_like; N-terminal Src homology 2 (nSH2) domain found in p85

cd11909
Location:7 → 80

SH3_PI3K_p85beta; Src Homology 3 domain of the p85beta regulatory subunit of Class IA Phosphatidylinositol 3-kinases

cd12926
Location:431 → 591

iSH2_PIK3R2; Inter-Src homology 2 (iSH2) helical domain of Class IA Phosphoinositide 3-kinase Regulatory subunit 2, PIK3R2, also called p85beta

pfam16454
Location:430 → 589

PI3K_P85_iSH2; Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain

cl02570
Location:111 → 289

RhoGAP; RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when ...