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Pcx pyruvate carboxylase [ Mus musculus (house mouse) ]

Gene ID: 18563, updated on 16-Apr-2024

Summary

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Official Symbol
Pcxprovided by MGI
Official Full Name
pyruvate carboxylaseprovided by MGI
Primary source
MGI:MGI:97520
See related
Ensembl:ENSMUSG00000024892 AllianceGenome:MGI:97520
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Pc; Pcb
Summary
Predicted to enable anion binding activity; identical protein binding activity; and pyruvate carboxylase activity. Predicted to be involved in several processes, including carboxylic acid metabolic process; gluconeogenesis; and viral RNA genome packaging. Predicted to act upstream of or within lipid metabolic process. Located in mitochondrion. Is expressed in several structures, including alimentary system; genitourinary system; integumental system; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in pyruvate carboxylase deficiency disease. Orthologous to human PC (pyruvate carboxylase). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in mammary gland adult (RPKM 255.0), adrenal adult (RPKM 223.4) and 13 other tissues See more
Orthologs
human all
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Genomic context

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See Pcx in Genome Data Viewer
Location:
19 A; 19 4.07 cM
Exon count:
22
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 19 NC_000085.7 (4560500..4671780)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 19 NC_000085.6 (4510472..4621752)

Chromosome 19 - NC_000085.7Genomic Context describing neighboring genes Neighboring gene RIKEN cDNA A930001C03 gene Neighboring gene synaptotagmin XII Neighboring gene RIKEN cDNA 2010003K11 gene Neighboring gene STARR-positive B cell enhancer ABC_E778 Neighboring gene STARR-seq mESC enhancer starr_45350 Neighboring gene leucine rich repeat and fibronectin type III domain containing 4 Neighboring gene microRNA 6986 Neighboring gene TOP6B like initiator of meiotic double strand breaks Neighboring gene Ras converting CAAX endopeptidase 1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Targeting pyruvate carboxylase reduces gluconeogenesis and adiposity and improves insulin resistance. Kumashiro N, et al. Diabetes, 2013 Jul. PMID 23423574, Free PMC Article
  2. Mild mitochondrial uncoupling does not affect mitochondrial biogenesis but downregulates pyruvate carboxylase in adipocytes: role for triglyceride content reduction. De Pauw A, et al. Am J Physiol Endocrinol Metab, 2012 May 15. PMID 22354779
  3. Reduction of islet pyruvate carboxylase activity might be related to the development of type 2 diabetes mellitus in Agouti-K mice. Han J, et al. J Endocrinol, 2010 Feb. PMID 19910451, Free PMC Article
  4. Structural and promoter regions of the murine pyruvate carboxylase gene. Jitrapakdee S, et al. Biochem Biophys Res Commun, 2001 Sep 21. PMID 11554743
  5. Adipose pyruvate carboxylase: amino acid sequence and domain structure deduced from cDNA sequencing. Zhang J, et al. Proc Natl Acad Sci U S A, 1993 Mar 1. PMID 8446588, Free PMC Article

See all (64) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Requirement of hepatic pyruvate carboxylase during fasting, high fat, and ketogenic diet.
    Title: Requirement of hepatic pyruvate carboxylase during fasting, high fat, and ketogenic diet.
  2. Pyruvate-Carboxylase-Mediated Anaplerosis Promotes Antioxidant Capacity by Sustaining TCA Cycle and Redox Metabolism in Liver.
    Title: Pyruvate-Carboxylase-Mediated Anaplerosis Promotes Antioxidant Capacity by Sustaining TCA Cycle and Redox Metabolism in Liver.
  3. the MDM2-p53-PC signalling axis links mitochondrial metabolism to insulin secretion and glucose homeostasis, and could represent a therapeutic target in diabetes.
    Title: The MDM2-p53-pyruvate carboxylase signalling axis couples mitochondrial metabolism to glucose-stimulated insulin secretion in pancreatic β-cells.
  4. These findings indicate that PC-mediated anaplerosis in early-stage NSCLC is required for tumor survival and proliferation.
    Title: Pyruvate carboxylase is critical for non-small-cell lung cancer proliferation.
  5. TR4-mediated enhancement of fatty acid synthesis in adipocytes requires increased expression of PC gene
    Title: TR4 promotes fatty acid synthesis in 3T3-L1 adipocytes by activation of pyruvate carboxylase expression.
  6. Repression of pyruvate carboxylase could be interpreted as a survival tactic of repressing anabolic pathways to support a redirection of limited carbon units to catabolism rather than storage
    Title: Mild mitochondrial uncoupling does not affect mitochondrial biogenesis but downregulates pyruvate carboxylase in adipocytes: role for triglyceride content reduction.
  7. Three GC boxes within the proximal promoter of the murine pyruvate carboxylase gene bind Sp1/Sp3
    Title: Functional characterization of the proximal promoter of the murine pyruvate carboxylase gene in hepatocytes: role of multiple gc boxes.
  8. CREB bind to -1639/-1631 cyclic AMP responsive element of mouse PC gene in vitro and in vivo, respectively.
    Title: Identification of the cyclic AMP responsive element (CRE) that mediates transcriptional regulation of the pyruvate carboxylase gene in HepG2 cells.
  9. Islet PC might play a role in the development of type 2 diabetes mellitus where reduction of PC activity might be a consequence of mild hyperglycemia and a cause for severe hyperglycemia.
    Title: Reduction of islet pyruvate carboxylase activity might be related to the development of type 2 diabetes mellitus in Agouti-K mice.
  10. Focuses on cloning of human pyruvate carboxylase but also studies pyruvate carboxylase levels in mouse preadipocyte differentiation.
    Title: Molecular cloning of a cDNA for human pyruvate carboxylase. Structural relationship to other biotin-containing carboxylases and regulation of mRNA content in differentiating preadipocytes.
Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (3)  1 citation
  • Endonuclease-mediated (3) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding ISO
Inferred from Sequence Orthology
more info
  
enables biotin binding ISO
Inferred from Sequence Orthology
more info
  
enables carboxylic acid binding ISO
Inferred from Sequence Orthology
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables pyruvate carboxylase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables pyruvate carboxylase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables pyruvate carboxylase activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in NADH metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in NADP metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in gluconeogenesis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in gluconeogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in gluconeogenesis ISO
Inferred from Sequence Orthology
more info
  
involved_in glyceraldehyde-3-phosphate biosynthetic process ISO
Inferred from Sequence Orthology
more info
 PubMed 
acts_upstream_of_or_within lipid metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of gene expression ISO
Inferred from Sequence Orthology
more info
  
involved_in oxaloacetate metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation by host of viral process ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of phospholipid biosynthetic process ISO
Inferred from Sequence Orthology
more info
  
involved_in pyruvate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in pyruvate metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in viral RNA genome packaging ISO
Inferred from Sequence Orthology
more info
  
involved_in viral release from host cell ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrial inner membrane HDA PubMed 
is_active_in mitochondrial matrix IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mitochondrion HDA PubMed 
located_in mitochondrion IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mitochondrion ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
pyruvate carboxylase, mitochondrial
Names
pyruvate decarboxylase
pyruvic carboxylase
NP_001156418.1
NP_032823.2
XP_006531741.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001162946.1 → NP_001156418.1  pyruvate carboxylase, mitochondrial isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AA619324, AK077665, AK148328, BG087228, BY230152, BY293312
    Consensus CDS
    CCDS50352.1
    UniProtKB/TrEMBL
    E9QPD7, Q3T9S7, Q3TCQ3
    Related
    ENSMUSP00000063825.7, ENSMUST00000068004.13
    Conserved Domains (1) summary
    PRK12999
    Location:34 → 1179
    PRK12999; pyruvate carboxylase; Reviewed

  2. NM_008797.3 → NP_032823.2  pyruvate carboxylase, mitochondrial isoform 2

    See identical proteins and their annotated locations for NP_032823.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
    Source sequence(s)
    AA619324, AK077665, AK148328, BG087228, BY059816, BY139237
    Consensus CDS
    CCDS29430.1
    UniProtKB/Swiss-Prot
    Q05920
    UniProtKB/TrEMBL
    G5E8R3
    Related
    ENSMUSP00000109456.3, ENSMUST00000113825.4
    Conserved Domains (1) summary
    PRK12999
    Location:33 → 1178
    PRK12999; pyruvate carboxylase; Reviewed

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000085.7 Reference GRCm39 C57BL/6J

    Range
    4560500..4671780
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006531678.5 → XP_006531741.1  pyruvate carboxylase, mitochondrial isoform X1

    See identical proteins and their annotated locations for XP_006531741.1

    UniProtKB/Swiss-Prot
    Q05920
    UniProtKB/TrEMBL
    G5E8R3
    Related
    ENSMUSP00000153479.2, ENSMUST00000224726.3
    Conserved Domains (1) summary
    PRK12999
    Location:33 → 1178
    PRK12999; pyruvate carboxylase; Reviewed
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q05920.1 GenPept UniProtKB/Swiss-Prot:Q05920

Gene LinkOut

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