Oprl1 opioid receptor-like 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Oprl1provided by MGI
Official Full Name: opioid receptor-like 1provided by MGI
Primary source: MGI:MGI:97440
See related: Ensembl:ENSMUSG00000027584 AllianceGenome:MGI:97440
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: NOP; KOR3; ORGC; ORL1; Oprl; XOR1; morc; KOR-3; LC132; MOR-C
Summary: The protein encoded by this gene is a member of the 7 transmembrane-spanning G protein-coupled receptor family, and functions as a receptor for the endogenous, opioid-related neuropeptide, nociceptin/orphanin FQ. This receptor-ligand system modulates a variety of biological functions and neurobehavior, including stress responses and anxiety behavior, learning and memory, locomotor activity, and inflammatory and immune responses. Alternatively spliced transcript variants have been described for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017]
Expression: Biased expression in whole brain E14.5 (RPKM 16.2), CNS E18 (RPKM 14.7) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 H4; 2 103.74 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (181356585..181362778)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (181715000..181720985)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Adverse maternal environment alters Oprl1 variant expression in mouse hippocampus.
Title: Adverse maternal environment alters Oprl1 variant expression in mouse hippocampus.
Behavioral and neurochemical effects of nociceptin/orphanin FQ receptor activation in the social defeat protocol.
Title: Behavioral and neurochemical effects of nociceptin/orphanin FQ receptor activation in the social defeat protocol.
Peripheral ablation of type III adenylyl cyclase induces hyperalgesia and eliminates KOR-mediated analgesia in mice.
Title: Peripheral ablation of type III adenylyl cyclase induces hyperalgesia and eliminates KOR-mediated analgesia in mice.
nociceptin opioid receptor agonists are promising drugs for treating pain in sickle cell anemia.
Title: Small-molecule nociceptin receptor agonist ameliorates mast cell activation and pain in sickle mice.
These knock-in mice have NOP receptors that function both in vitro and in vivo and have provided a detailed characterization of NOP receptors in brain, spinal cord, and DRG neurons.
Title: Knock-In Mice with NOP-eGFP Receptors Identify Receptor Cellular and Regional Localization.
Oprl1 is associated with amygdala function, fear processing, and posttraumatic stress disorder symptoms.
Title: Amygdala-dependent fear is regulated by Oprl1 in mice and humans with PTSD.
the present results suggest that the endogenous OFQ/N/NOP receptor system may modulate the development of cocaine-induced locomotor sensitization.
Title: The role of NOP receptors in psychomotor stimulation and locomotor sensitization induced by cocaine and amphetamine in mice.
Data suggest that NOPR and MOPR (mu-type opioid receptor) in spinal cord independently drive antinociception; NOPR/MOPR ligands attenuate neuropathic/inflammatory pain and have high antinociceptive potency with low tolerance development.
Title: Effects of spinally administered bifunctional nociceptin/orphanin FQ peptide receptor/μ-opioid receptor ligands in mouse models of neuropathic and inflammatory pain.
Data suggest that nociceptin receptor/nociceptin signaling plays a role in the disease pathogenesis of inflammatory bowel diseases.
Title: Nociceptin/orphanin FQ inhibition with SB612111 ameliorates dextran sodium sulfate-induced colitis.
The results of this study demonstrated the contribution of the NOP receptor in controlling food intake and meal pattern, as well as glutamate release and GIRK1 channel activity at POMC synapses.
Title: The role of the NOP receptor in regulating food intake, meal pattern, and the excitability of proopiomelanocortin neurons.

Submit: New GeneRIF Correction See all GeneRIFs (23)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (4) 1 citation
Endonuclease-mediated (3)

General gene information

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Markers

RH140403 (e-PCR)
- UniSTS:222478

24.MMHAP91FLH3.seq (e-PCR)
- UniSTS:123663

Oprl1 (e-PCR), detects polymorphism
- UniSTS:496032

Oprl1 (e-PCR), detects polymorphism
- UniSTS:499104

AF043276 (e-PCR)
- UniSTS:185523

Oprl (e-PCR), detects polymorphism
- UniSTS:144087

Oprl (e-PCR), detects polymorphism
- UniSTS:144088

U09421 (e-PCR)
- UniSTS:125660

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables G protein-coupled opioid receptor activity	IEA Inferred from Electronic Annotation more info
enables G protein-coupled receptor activity	ISO Inferred from Sequence Orthology more info
enables neuropeptide binding	IBA Inferred from Biological aspect of Ancestor more info
enables nociceptin receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables nociceptin receptor activity	IGI Inferred from Genetic Interaction more info	PubMed
enables nociceptin receptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables nociceptin receptor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within G protein-coupled opioid receptor signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within behavior	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within_positive_effect calcium-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in conditioned place preference	ISO Inferred from Sequence Orthology more info
involved_in eating behavior	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of blood pressure	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cAMP-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in neuron-neuron synaptic transmission	IGI Inferred from Genetic Interaction more info	PubMed
involved_in neuropeptide signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of urine volume	ISO Inferred from Sequence Orthology more info
involved_in regulation of locomotor rhythm	ISO Inferred from Sequence Orthology more info
involved_in sensory perception of pain	IBA Inferred from Biological aspect of Ancestor more info
involved_in sensory perception of pain	IGI Inferred from Genetic Interaction more info	PubMed
involved_in sensory perception of pain	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in synaptic membrane	IGI Inferred from Genetic Interaction more info	PubMed

General protein information

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Preferred Names: nociceptin receptor

Names: K3 opiate receptor; N/OFQ receptor; kappa-type 3 opioid receptor; nociceptin/ orphaninFQ receptor

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001252565.2 → NP_001239494.1 nociceptin receptor isoform 1

See identical proteins and their annotated locations for NP_001239494.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) uses an alternate 5' non-coding exon; therefore, differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1).

Source sequence(s)

AL845173

Consensus CDS

CCDS17222.1

UniProtKB/Swiss-Prot

P35377, Q60645, Q8VI74, Q9QUT5, Q9Z2M8, Q9Z2M9, Q9Z2N0

UniProtKB/TrEMBL

Q542U1, Q80WU7

Related

ENSMUSP00000104399.2, ENSMUST00000108768.8

Conserved Domains (1) summary

cd15092
Location:49 → 327

7tmA_NOFQ_opioid_R; nociceptin/orphanin FQ peptide receptor, member of the class A family of seven-transmembrane G protein-coupled receptors
NM_001318919.1 → NP_001305848.1 nociceptin receptor isoform 1x

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon), while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the longer, C-terminally extended isoform (1x). As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), the location of the upstream UGA stop codon in the longer isoform is denoted by an 'X'.

Source sequence(s)

AL845173, BC050885, BY267034

Consensus CDS

CCDS89611.1

UniProtKB/TrEMBL

A0A5F8MPZ9, Q80WU7

Related

ENSMUSP00000159197.2, ENSMUST00000238942.2

Conserved Domains (1) summary

cd15092
Location:49 → 327

7tmA_NOFQ_opioid_R; nociceptin/orphanin FQ peptide receptor, member of the class A family of seven-transmembrane G protein-coupled receptors
NM_001318920.2 → NP_001305849.1 nociceptin receptor isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (3), also known as KOR-3d, uses an alternate, in-frame donor splice site at the first coding exon compared to variant 1. The resulting isoform (2) is shorter, missing a 5 aa protein segment compared to isoform 1. Variants 3 and 9 both encode the same isoform (2).

Source sequence(s)

AL845173

UniProtKB/TrEMBL

Q80WU7

Conserved Domains (1) summary

cd15092
Location:49 → 322

7tmA_NOFQ_opioid_R; nociceptin/orphanin FQ peptide receptor, member of the class A family of seven-transmembrane G protein-coupled receptors
NM_001318922.2 → NP_001305851.1 nociceptin receptor isoform 3

Status: REVIEWED

Description

Transcript Variant: This variant (4), also known as KOR-3a, contains an additional internal exon, which results in translation initiation from an alternate start codon compared to variant 1. The encoded isoform (3) is shorter with a distinct N-terminus compared to isoform 1. Variants 4 and 10 both encode the same isoform (3).

Source sequence(s)

AL845173

Consensus CDS

CCDS84606.1

UniProtKB/TrEMBL

B0R0C0

Related

ENSMUSP00000104394.2, ENSMUST00000108763.8

Conserved Domains (2) summary

cd14964
Location:70 → 91

7tm_GPCRs; TM helix 2 [structural motif]

cl28897
Location:61 → 313

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily
NM_001318923.2 → NP_001305852.1 nociceptin receptor isoform 4

Status: REVIEWED

Description

Transcript Variant: This variant (5) uses an alternate donor splice site and contains an additional internal exon, which results in translation initiation from an alternate start codon compared to variant 1. The encoded isoform (4) is shorter with a distinct N-terminus compared to isoform 1.

Source sequence(s)

AL845173

Conserved Domains (2) summary

cd14964
Location:65 → 86

7tm_GPCRs; TM helix 2 [structural motif]

cl28897
Location:56 → 308

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily
NM_001318924.2 → NP_001305853.1 nociceptin receptor isoform 5

Status: REVIEWED

Description

Transcript Variant: This variant (6), also known as KOR-3b, contains an additional internal exon, which results in translation initiation from an in-frame, downstream start codon compared to variant 1. The encoded isoform (5) has a shorter N-terminus compared to isoform 1. Variants 6, 7, and 11-13 all encode the same isoform (5).

Source sequence(s)

AL845173

Conserved Domains (2) summary

cd14964
Location:5 → 31

7tm_GPCRs; TM helix 2 [structural motif]

cl28897
Location:1 → 249

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily
NM_001318925.2 → NP_001305854.1 nociceptin receptor isoform 5

Status: REVIEWED

Description

Transcript Variant: This variant (7), also known as KOR-3c, contains an additional internal exon, which results in translation initiation from an in-frame, downstream start codon compared to variant 1. The encoded isoform (5) has a shorter N-terminus compared to isoform 1. Variants 6, 7, and 11-13 all encode the same isoform (5).

Source sequence(s)

AL845173

Conserved Domains (2) summary

cd14964
Location:5 → 31

7tm_GPCRs; TM helix 2 [structural motif]

cl28897
Location:1 → 249

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily
NM_001420497.1 → NP_001407426.1 nociceptin receptor isoform 2

Status: REVIEWED

Source sequence(s)

AL845173
NM_001420498.1 → NP_001407427.1 nociceptin receptor isoform 3

Status: REVIEWED

Source sequence(s)

AL845173

UniProtKB/TrEMBL

B0R0C0
NM_001420499.1 → NP_001407428.1 nociceptin receptor isoform 5

Status: REVIEWED

Source sequence(s)

AL845173
NM_001420500.1 → NP_001407429.1 nociceptin receptor isoform 5

Status: REVIEWED

Source sequence(s)

AL845173
NM_001420501.1 → NP_001407430.1 nociceptin receptor isoform 5

Status: REVIEWED

Source sequence(s)

AL845173
NM_011012.6 → NP_035142.1 nociceptin receptor isoform 1

See identical proteins and their annotated locations for NP_035142.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (1). Variants 1 and 2 encode the same isoform.

Source sequence(s)

AL845173

Consensus CDS

CCDS17222.1

UniProtKB/Swiss-Prot

P35377, Q60645, Q8VI74, Q9QUT5, Q9Z2M8, Q9Z2M9, Q9Z2N0

UniProtKB/TrEMBL

Q542U1, Q80WU7

Related

ENSMUSP00000071513.4, ENSMUST00000071585.10

Conserved Domains (1) summary

cd15092
Location:49 → 327

7tmA_NOFQ_opioid_R; nociceptin/orphanin FQ peptide receptor, member of the class A family of seven-transmembrane G protein-coupled receptors

RNA

NR_134926.2 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (8) lacks two consecutive internal exons compared to variant 1. It is represented as non-coding because it lacks a large portion of the coding region, including the translational start codon, found in variant 1.

Source sequence(s)

AL845173

Related

ENSMUST00000126835.2

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

181356585..181362778

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030248361.2 → XP_030104221.1 nociceptin receptor isoform X3

UniProtKB/TrEMBL

B0R0C0

Conserved Domains (2) summary

cd14964
Location:70 → 91

7tm_GPCRs; TM helix 2 [structural motif]

cl28897
Location:61 → 313

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily
XM_017316325.2 → XP_017171814.1 nociceptin receptor isoform X1

UniProtKB/Swiss-Prot

P35377, Q60645, Q8VI74, Q9QUT5, Q9Z2M8, Q9Z2M9, Q9Z2N0

UniProtKB/TrEMBL

Q542U1, Q80WU7

Conserved Domains (1) summary

cd15092
Location:49 → 327

7tmA_NOFQ_opioid_R; nociceptin/orphanin FQ peptide receptor, member of the class A family of seven-transmembrane G protein-coupled receptors
XM_036159470.1 → XP_036015363.1 nociceptin receptor isoform X2

UniProtKB/TrEMBL

Q80WU7

Conserved Domains (1) summary

cd15092
Location:49 → 322

7tmA_NOFQ_opioid_R; nociceptin/orphanin FQ peptide receptor, member of the class A family of seven-transmembrane G protein-coupled receptors
XM_036159475.1 → XP_036015368.1 nociceptin receptor isoform X4

Conserved Domains (2) summary

cd14964
Location:5 → 31

7tm_GPCRs; TM helix 2 [structural motif]

cl28897
Location:1 → 249

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily
XM_030248365.2 → XP_030104225.1 nociceptin receptor isoform X4

Conserved Domains (2) summary

cd14964
Location:5 → 31

7tm_GPCRs; TM helix 2 [structural motif]

cl28897
Location:1 → 249

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily
XM_036159476.1 → XP_036015369.1 nociceptin receptor isoform X4

Conserved Domains (2) summary

cd14964
Location:5 → 31

7tm_GPCRs; TM helix 2 [structural motif]

cl28897
Location:1 → 249

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily
XM_017316330.1 → XP_017171819.1 nociceptin receptor isoform X4

Conserved Domains (2) summary

cd14964
Location:5 → 31

7tm_GPCRs; TM helix 2 [structural motif]

cl28897
Location:1 → 249

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily
XM_030248366.1 → XP_030104226.1 nociceptin receptor isoform X4

Conserved Domains (2) summary

cd14964
Location:5 → 31

7tm_GPCRs; TM helix 2 [structural motif]

cl28897
Location:1 → 249

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily