Nid2 nidogen 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nid2provided by MGI
Official Full Name: nidogen 2provided by MGI
Primary source: MGI:MGI:1298229
See related: Ensembl:ENSMUSG00000021806 AllianceGenome:MGI:1298229
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ly111; NID-2; nidogen-2; entactin-2
Summary: Predicted to enable calcium ion binding activity. Predicted to be an extracellular matrix structural constituent. Acts upstream of or within cell-matrix adhesion. Located in basement membrane. Is expressed in several structures, including alimentary system; extraembryonic component; genitourinary system; nervous system; and respiratory system. Orthologous to human NID2 (nidogen 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in limb E14.5 (RPKM 30.8), subcutaneous fat pad adult (RPKM 23.2) and 24 other tissues See more
Orthologs: human all
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Genomic context

Location:: 14 A3; 14 10.73 cM

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (19801325..19861855)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (19751257..19811787)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nidogen-2 is a Novel Endogenous Ligand of LGR4 to Inhibit Vascular Calcification.
Title: Nidogen-2 is a Novel Endogenous Ligand of LGR4 to Inhibit Vascular Calcification.
Nidogen-2, a protein shown to be expressed intracellularly and for secretion by pre-adipocytes, was shown to interact, through its globular G3 domain, with TRAP 5a in vitro.
Title: The growth factor-like adipokine tartrate-resistant acid phosphatase 5a interacts with the rod G3 domain of adipocyte-produced nidogen-2.
finding demonstrates that nidogens, although homologous proteins, exert their effect on BM assembly through different binding domains, which may in turn result in alterations of BM structure and functions
Title: Different domains in nidogen-1 and nidogen-2 drive basement membrane formation in skin organotypic cocultures.
show that loss of nidogen 2, but not of nidogen 1, promotes lung metastasis of melanoma cells. Histological and ultrastructural analysis of nidogen 1- and 2-deficient lungs did not reveal differences in morphology and ultrastructure of basement membrane
Title: Absence of the basement membrane component nidogen 2, but not of nidogen 1, results in increased lung metastasis in mice.
Basement membrane deposition of nidogen 1 but not nidogen 2 requires the nidogen binding module of the laminin gamma1 chain.
Title: Basement membrane deposition of nidogen 1 but not nidogen 2 requires the nidogen binding module of the laminin gamma1 chain.
Neuromuscular junctions in mutant mice lacking nidogen-2 appear normal at birth, but become topologically abnormal as they mature
Title: A synaptic nidogen: developmental regulation and role of nidogen-2 at the neuromuscular junction.
In capillaries, basement membrane formation is severely impaired in the absence of nidogen-1 and -2, showing an irregular, patchy distribution and a dramatically reduced deposition of collagen IV, perlecan, and particularly laminin-411.
Title: Basement membranes in skin are differently affected by lack of nidogen 1 and 2.
nidogen-2 is equivalent to nidogen-1 in skin-organotypic coculture, and both can promote the development of a functional basement membrane zone
Title: Lack of nidogen-1 and -2 prevents basement membrane assembly in skin-organotypic coculture.
Nid2 is essential for late stages of lung development and for maintenance and/or integrity of cardiac tissue
Title: Compound genetic ablation of nidogen 1 and 2 causes basement membrane defects and perinatal lethality in mice.
nidogen-1 and -2 have roles in development of the ectodermal basement membrane of the limb bud
Title: Loss of nidogen-1 and -2 results in syndactyly and changes in limb development.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1) 1 citation
Targeted (1)
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Nid2 (e-PCR), detects polymorphism
- UniSTS:472867

NoName (e-PCR)
- UniSTS:237133

AB017202 (e-PCR)
- UniSTS:209002

AW547149 (e-PCR)
- UniSTS:180617

PMC135501P1 (e-PCR)
- UniSTS:270756

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables extracellular matrix structural constituent	RCA inferred from Reviewed Computational Analysis more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in cell-matrix adhesion	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cell-matrix adhesion	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cell-matrix adhesion	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in basement membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in basement membrane	IDA Inferred from Direct Assay more info	PubMed
located_in basement membrane	IPI Inferred from Physical Interaction more info	PubMed
located_in basement membrane	ISO Inferred from Sequence Orthology more info
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in collagen-containing extracellular matrix	HDA more info	PubMed
located_in extracellular matrix	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in extracellular region	TAS Traceable Author Statement more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: nidogen-2

Names: entactin 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008695.2 → NP_032721.2 nidogen-2 precursor

See identical proteins and their annotated locations for NP_032721.2

Status: VALIDATED

Source sequence(s)

AK052839, AK164258, AY074820, BY143483

Consensus CDS

CCDS36813.1

UniProtKB/Swiss-Prot

O88322, Q7TQF0

UniProtKB/TrEMBL

Q3TPN0, Q8CFA3

Related

ENSMUSP00000022340.4, ENSMUST00000022340.5

Conserved Domains (6) summary

smart00135
Location:1206 → 1248

LY; Low-density lipoprotein-receptor YWTD domain

smart00539
Location:108 → 275

NIDO; Extracellular domain of unknown function in nidogen (entactin) and hypothetical proteins

cd00191
Location:1047 → 1112

TY; Thyroglobulin type I repeats.; The N-terminal region of human thyroglobulin contains 11 type-1 repeats TY repeats are proposed to be inhibitors of cysteine proteases

pfam07645
Location:824 → 865

EGF_CA; Calcium-binding EGF domain

pfam12947
Location:919 → 952

EGF_3; EGF domain

cl00186
Location:548 → 780

nidG2; Nidogen, G2 domain; Nidogen is an important component of the basement membrane, an extracellular sheet-like matrix. Nidogen is a multifunctional protein that interacts with many other basement membrane proteins, like collagen, perlecan, lamin, and has a ...