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Me1 malic enzyme 1, NADP(+)-dependent, cytosolic [ Mus musculus (house mouse) ]

Gene ID: 17436, updated on 11-Apr-2024

Summary

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Official Symbol
Me1provided by MGI
Official Full Name
malic enzyme 1, NADP(+)-dependent, cytosolicprovided by MGI
Primary source
MGI:MGI:97043
See related
Ensembl:ENSMUSG00000032418 AllianceGenome:MGI:97043
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Mod1; Mdh-1; Mod-1; D9Ertd267e
Summary
Enables malate dehydrogenase (decarboxylating) (NADP+) activity. Predicted to be involved in carboxylic acid metabolic process; regulation of NADP metabolic process; and response to hormone. Located in mitochondrion. Is expressed in several structures, including cardiovascular system; central nervous system; cranium; eye; and genitourinary system. Orthologous to human ME1 (malic enzyme 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in subcutaneous fat pad adult (RPKM 140.3), mammary gland adult (RPKM 130.2) and 16 other tissues See more
Orthologs
human all
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Genomic context

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Location:
9 E3.1; 9 46.58 cM
Exon count:
16
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 9 NC_000075.7 (86463416..86577967, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 9 NC_000075.6 (86581363..86695914, complement)

Chromosome 9 - NC_000075.7Genomic Context describing neighboring genes Neighboring gene DOP1 leucine zipper like protein A Neighboring gene STARR-positive B cell enhancer ABC_E9332 Neighboring gene phosphoglucomutase 3 Neighboring gene STARR-positive B cell enhancer ABC_E10572 Neighboring gene RWD domain containing 2A Neighboring gene STARR-seq mESC enhancer starr_24835 Neighboring gene RIKEN cDNA A330041J22 gene Neighboring gene predicted gene, 22138 Neighboring gene serine protease 35

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Malic Enzyme 1 (ME1) Promotes Adiposity and Hepatic Steatosis and Induces Circulating Insulin and Leptin in Obese Female Mice. Simmen FA, et al. Int J Mol Sci, 2023 Apr 1. PMID 37047583, Free PMC Article
  2. Malic enzyme 1 is important for uterine decidualization in response to progesterone/cAMP/PKA/HB-EGF pathway. Yu HF, et al. FASEB J, 2020 Mar. PMID 31944402
  3. Malic enzyme tracers reveal hypoxia-induced switch in adipocyte NADPH pathway usage. Liu L, et al. Nat Chem Biol, 2016 May. PMID 26999781, Free PMC Article
  4. Enhanced gastrointestinal expression of cytosolic malic enzyme (ME1) induces intestinal and liver lipogenic gene expression and intestinal cell proliferation in mice. Al-Dwairi A, et al. PLoS One, 2014. PMID 25402228, Free PMC Article
  5. Cytosolic malic enzyme 1 (ME1) mediates high fat diet-induced adiposity, endocrine profile, and gastrointestinal tract proliferation-associated biomarkers in male mice. Al-Dwairi A, et al. PLoS One, 2012. PMID 23056418, Free PMC Article

See all (131) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Malic Enzyme 1 (ME1) Promotes Adiposity and Hepatic Steatosis and Induces Circulating Insulin and Leptin in Obese Female Mice.
    Title: Malic Enzyme 1 (ME1) Promotes Adiposity and Hepatic Steatosis and Induces Circulating Insulin and Leptin in Obese Female Mice.
  2. Malic enzyme 1 is important for uterine decidualization in response to progesterone/cAMP/PKA/HB-EGF pathway.
    Title: Malic enzyme 1 is important for uterine decidualization in response to progesterone/cAMP/PKA/HB-EGF pathway.
  3. Data suggest that Ad4BP plays role in regulation of intracellular NADPH concentration via transcription of Me1 and Mthfd2 genes in adrenocortical cells. (Ad4BP = nuclear receptor subfamily 5 group A member 1; Me1 = malic enzyme 1; Mthfd2 = bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase)
    Title: Role of Ad4-binding protein/steroidogenic factor 1 in regulating NADPH production in adrenocortical Y-1 cells.
  4. This study showed that (2)H-labeled tracers enable dissection of NADPH production routes across cell types and environmental conditions.
    Title: Malic enzyme tracers reveal hypoxia-induced switch in adipocyte NADPH pathway usage.
  5. ME1 expression was found to be mutant-KRAS-associated in an NSCLC mouse model.
    Title: Mutant KRAS associated malic enzyme 1 expression is a predictive marker for radiation therapy response in non-small cell lung cancer.
  6. p53 represses the expression of the tricarboxylic-acid-cycle-associated malic enzymes ME1 and ME2 in human and mouse cells
    Title: Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence.
  7. although ME1 overexpression augments anaplerosis and glucose stimulated insulin secretion in INS-1 832/13 cells, it is not likely involved in methyl succinate and glucose stimulated insulin secretion in pancreatic islets
    Title: Role for malic enzyme, pyruvate carboxylation, and mitochondrial malate import in glucose-stimulated insulin secretion.
  8. cytosolic or mitochondrial isoforms of malic enzyme do not affect glucose-stimulated insulin secretion from rodent islets
    Title: Silencing of cytosolic or mitochondrial isoforms of malic enzyme has no effect on glucose-stimulated insulin secretion from rodent islets.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Spontaneous (1)  1 citation
  • Endonuclease-mediated (4) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC91072

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables NAD binding IEA
Inferred from Electronic Annotation
more info
  
enables NADP binding ISO
Inferred from Sequence Orthology
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables magnesium ion binding ISO
Inferred from Sequence Orthology
more info
  
enables malate dehydrogenase (decarboxylating) (NAD+) activity IEA
Inferred from Electronic Annotation
more info
  
enables malate dehydrogenase (decarboxylating) (NADP+) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables malate dehydrogenase (decarboxylating) (NADP+) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables malate dehydrogenase (decarboxylating) (NADP+) activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables malate dehydrogenase (decarboxylating) (NADP+) activity ISO
Inferred from Sequence Orthology
more info
  
enables malate dehydrogenase (decarboxylating) (NADP+) activity ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
enables malic enzyme activity ISO
Inferred from Sequence Orthology
more info
  
enables malic enzyme activity ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
enables manganese ion binding ISO
Inferred from Sequence Orthology
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables oxaloacetate decarboxylase activity ISO
Inferred from Sequence Orthology
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in NADH metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in NADP metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in malate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in malate metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in malate metabolic process ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
involved_in protein homotetramerization ISO
Inferred from Sequence Orthology
more info
  
involved_in pyruvate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of NADP metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in response to hormone ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrion HDA PubMed 
is_active_in mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in mitochondrion ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
NADP-dependent malic enzyme
Names
NADP-ME
malic enzyme, supernatant
NP_001185862.1
NP_032641.2
XP_011240968.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001198933.1 → NP_001185862.1  NADP-dependent malic enzyme isoform 2

    See identical proteins and their annotated locations for NP_001185862.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) has an alternate exon at its 5' UTR and 5' coding region, compared to variant 1, which results in a protein (isoform 2) with a shorter and distinct N-terminus, compared to isoform 1.
    Source sequence(s)
    AK077968, AK163403, AV363687
    Consensus CDS
    CCDS81047.1
    UniProtKB/TrEMBL
    Q3TQP6, Q921S3
    Related
    ENSMUSP00000140887.2, ENSMUST00000185374.2
    Conserved Domains (3) summary
    cd05312
    Location:250 → 527
    NAD_bind_1_malic_enz; NAD(P) binding domain of malic enzyme (ME), subgroup 1
    PLN03129
    Location:7 → 531
    PLN03129; NADP-dependent malic enzyme; Provisional
    pfam00390
    Location:59 → 240
    malic; Malic enzyme, N-terminal domain

  2. NM_008615.2 → NP_032641.2  NADP-dependent malic enzyme isoform 1

    See identical proteins and their annotated locations for NP_032641.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the longer isoform (1).
    Source sequence(s)
    AK077968, AV363687, CJ070715
    Consensus CDS
    CCDS23383.1
    UniProtKB/Swiss-Prot
    P06801, Q9DBF9
    UniProtKB/TrEMBL
    Q921S3
    Related
    ENSMUSP00000034989.9, ENSMUST00000034989.15
    Conserved Domains (3) summary
    cd05312
    Location:270 → 547
    NAD_bind_1_malic_enz; NAD(P) binding domain of malic enzyme (ME), subgroup 1
    PLN03129
    Location:2 → 551
    PLN03129; NADP-dependent malic enzyme; Provisional
    pfam00390
    Location:79 → 260
    malic; Malic enzyme, N-terminal domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000075.7 Reference GRCm39 C57BL/6J

    Range
    86463416..86577967 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011242666.2 → XP_011240968.1  NADP-dependent malic enzyme isoform X1

    UniProtKB/TrEMBL
    Q921S3
    Conserved Domains (1) summary
    PLN03129
    Location:1 → 476
    PLN03129; NADP-dependent malic enzyme; Provisional
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P06801.2 GenPept UniProtKB/Swiss-Prot:P06801

Gene LinkOut

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