Pias2 protein inhibitor of activated STAT 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pias2provided by MGI
Official Full Name: protein inhibitor of activated STAT 2provided by MGI
Primary source: MGI:MGI:1096566
See related: Ensembl:ENSMUSG00000025423 AllianceGenome:MGI:1096566
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: DIP; Dib; Miz1; SIZ2; ARIP3; PIASxb; PIASxbeta; PIASxalpha; PIASxalpha6
Summary: Enables DNA binding activity; SUMO transferase activity; and ubiquitin protein ligase binding activity. Acts upstream of or within several processes, including protein sumoylation; regulation of osteoblast differentiation; and transcription, DNA-templated. Located in nuclear body. Is expressed in several structures, including branchial arch; central nervous system; early conceptus; embryo mesenchyme; and genitourinary system. Orthologous to human PIAS2 (protein inhibitor of activated STAT 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in testis adult (RPKM 36.6), CNS E14 (RPKM 8.7) and 22 other tissues See more
Orthologs: human all
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Genomic context

Location:: 18 E3; 18 52.07 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	18	NC_000084.7 (77152883..77243406)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	18	NC_000084.6 (77065176..77155710)

Chromosome 18 - NC_000084.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Regulation of BCR-mediated Ca[2+] mobilization by MIZ1-TMBIM4 safeguards IgG1[+] GC B cell-positive selection.
Title: Regulation of BCR-mediated Ca(2+) mobilization by MIZ1-TMBIM4 safeguards IgG1(+) GC B cell-positive selection.
Suppression of Allergic Asthma by Loss of Function of Miz1-mediated Th1 Skewing.
Title: Suppression of Allergic Asthma by Loss of Function of Miz1-mediated Th1 Skewing.
A direct interaction between Pold2 and PIAS2 was confirmed by direct yeast two-hybrid.
Title: Identification of Pold2 as a novel interaction partner of protein inhibitor of activated STAT2.
Interaction of protein inhibitor of activated STAT 2 (PIAS2) with receptor of activated C kinase 1, RACK1.
Title: Interaction of protein inhibitor of activated STAT 2 (PIAS2) with receptor of activated C kinase 1, RACK1.
regulates interleukin-7 receptor signaling at early commitment stages of B cell differentiation
Title: Transcription factor miz-1 is required to regulate interleukin-7 receptor signaling at early commitment stages of B cell differentiation.
study provides the first line of evidence that PIASxbeta is indispensable for osteoblast differentiation and matrix mineralization, and that this signaling molecule is located between Runx2 and osterix.
Title: PIASxbeta is a key regulator of osterix transcriptional activity and matrix mineralization in osteoblasts.
a transcriptional cofactor for TFII-I
Title: The SUMO ubiquitin-protein isopeptide ligase family member Miz1/PIASxbeta /Siz2 is a transcriptional cofactor for TFII-I.
Miz-1 trans-actiates Nramp1 in mice
Title: Characterization of the murine Nramp1 promoter: requirements for transactivation by Miz-1.
role in activating natural resistance-associated protein 1 promoter
Title: c-Myc represses and Miz-1 activates the murine natural resistance-associated protein 1 promoter.
Miz-1 has a role in inducing apoptosis along with MAGE-A4
Title: A cleaved form of MAGE-A4 binds to Miz-1 and induces apoptosis in human cells.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1) 1 citation
Targeted (1)
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Pias2 (e-PCR), detects polymorphism
- UniSTS:527489

AI462206 (e-PCR)
- UniSTS:181152

RH139541 (e-PCR)
- UniSTS:221626

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables SUMO ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables SUMO ligase activity	ISO Inferred from Sequence Orthology more info
enables SUMO transferase activity	EXP Inferred from Experiment more info	PubMed
enables SUMO transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables SUMO transferase activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nuclear androgen receptor binding	ISO Inferred from Sequence Orthology more info	PubMed
enables nuclear estrogen receptor binding	ISO Inferred from Sequence Orthology more info
enables nuclear glucocorticoid receptor binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables transcription coregulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in androgen receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of androgen receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of dendrite morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in protein sumoylation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within protein sumoylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein sumoylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of androgen receptor signaling pathway	ISO Inferred from Sequence Orthology more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
colocalizes_with PML body	ISO Inferred from Sequence Orthology more info
part_of chromatin	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info	PubMed

General protein information

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Preferred Names: E3 SUMO-protein ligase PIAS2

Names: DAB2-interacting protein; Msx-interacting-zinc finger; RING-type E3 ubiquitin transferase PIAS2; androgen receptor-interacting protein 3; msx-interacting zinc finger protein; protein inhibitor of activated STAT x

NP_001157639.1

EC 2.3.2.27

NP_001157640.1

EC 2.3.2.27

NP_001157641.1

EC 2.3.2.27

NP_001157642.1

EC 2.3.2.27

NP_032628.3

EC 2.3.2.27

XP_006526486.1

EC 2.3.2.27

XP_006526488.1

EC 2.3.2.27

XP_036016910.1

EC 2.3.2.27

XP_036016911.1

EC 2.3.2.27

XP_036016912.1

EC 2.3.2.27

XP_036016913.1

EC 2.3.2.27

XP_036016914.1

EC 2.3.2.27

XP_036016916.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001164167.1 → NP_001157639.1 E3 SUMO-protein ligase PIAS2 isoform 2

See identical proteins and their annotated locations for NP_001157639.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1. This results in a frameshift and distinct C-terminus in isoform 2, compared to isoform 1.

Source sequence(s)

AK029716, AK032463, AK086653

UniProtKB/Swiss-Prot

Q8C5D8

UniProtKB/TrEMBL

Q3V3U5

Conserved Domains (3) summary

smart00513
Location:11 → 45

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

pfam02891
Location:342 → 390

zf-MIZ; MIZ/SP-RING zinc finger

pfam14324
Location:145 → 282

PINIT; PINIT domain
NM_001164168.1 → NP_001157640.1 E3 SUMO-protein ligase PIAS2 isoform 3

See identical proteins and their annotated locations for NP_001157640.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) contains an alternate exon in the 5' coding region and uses a downstream start codon, compared to variant 1. Isoform 3 has a shorter and distinct N-terminus, compared to isoform 1.

Source sequence(s)

AK032463, AK086653, BU704371

Consensus CDS

CCDS50324.1

UniProtKB/TrEMBL

G3UWE3

Related

ENSMUSP00000131485.2, ENSMUST00000168882.9

Conserved Domains (3) summary

smart00513
Location:4 → 38

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

pfam02891
Location:335 → 383

zf-MIZ; MIZ/SP-RING zinc finger

pfam14324
Location:138 → 275

PINIT; PINIT domain
NM_001164169.1 → NP_001157641.1 E3 SUMO-protein ligase PIAS2 isoform 4

Status: VALIDATED

Description

Transcript Variant: This variant (4) contains an alternate exon in the 3' coding region, resulting in a translational frameshift, compared to variant 1. The resulting protein (isoform 4) has a distinct C-terminus and is shorter than isoform 1.

Source sequence(s)

AC157991, AK014871, AK029716

UniProtKB/Swiss-Prot

Q8C5D8

Conserved Domains (3) summary

smart00513
Location:11 → 45

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

pfam02891
Location:342 → 390

zf-MIZ; MIZ/SP-RING zinc finger

pfam14324
Location:145 → 282

PINIT; PINIT domain
NM_001164170.1 → NP_001157642.1 E3 SUMO-protein ligase PIAS2 isoform 5

Status: VALIDATED

Description

Transcript Variant: This variant (5) has multiple differences in the coding region, compared to variant 1, one of which results in a translational frameshift. The resulting protein (isoform 5) has a distinct N- and C-terminus and is shorter than isoform 1.

Source sequence(s)

AC157991, AK014871, AK086653, BU704371

Consensus CDS

CCDS50323.1

UniProtKB/TrEMBL

F8WHS8

Related

ENSMUSP00000110424.3, ENSMUST00000114776.4

Conserved Domains (3) summary

smart00513
Location:4 → 38

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

pfam02891
Location:335 → 383

zf-MIZ; MIZ/SP-RING zinc finger

pfam14324
Location:138 → 275

PINIT; PINIT domain
NM_008602.4 → NP_032628.3 E3 SUMO-protein ligase PIAS2 isoform 1

See identical proteins and their annotated locations for NP_032628.3

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

AC157991, AK029716, AK032463

Consensus CDS

CCDS37866.1

UniProtKB/Swiss-Prot

O54987, Q8C384, Q8C5D8, Q8CDQ8, Q8K208, Q99JX5, Q9D5W7, Q9QZ63

Related

ENSMUSP00000110425.3, ENSMUST00000114777.10

Conserved Domains (3) summary

smart00513
Location:11 → 45

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

pfam02891
Location:342 → 390

zf-MIZ; MIZ/SP-RING zinc finger

pfam14324
Location:145 → 282

PINIT; PINIT domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000084.7 Reference GRCm39 C57BL/6J

Range

77152883..77243406

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006526423.4 → XP_006526486.1 E3 SUMO-protein ligase PIAS2 isoform X1

Conserved Domains (3) summary

smart00513
Location:11 → 45

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

cd16790
Location:343 → 390

SP-RING_PIAS; SP-RING finger found in protein inhibitor of activated signal transducer and activator of transcription (PIAS) proteins

pfam14324
Location:147 → 291

PINIT; PINIT domain
XM_036161018.1 → XP_036016911.1 E3 SUMO-protein ligase PIAS2 isoform X4

Conserved Domains (3) summary

smart00513
Location:11 → 45

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

cd16790
Location:343 → 390

SP-RING_PIAS; SP-RING finger found in protein inhibitor of activated signal transducer and activator of transcription (PIAS) proteins

pfam14324
Location:147 → 296

PINIT; PINIT domain
XM_036161019.1 → XP_036016912.1 E3 SUMO-protein ligase PIAS2 isoform X4

Conserved Domains (3) summary

smart00513
Location:11 → 45

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

cd16790
Location:343 → 390

SP-RING_PIAS; SP-RING finger found in protein inhibitor of activated signal transducer and activator of transcription (PIAS) proteins

pfam14324
Location:147 → 296

PINIT; PINIT domain
XM_036161020.1 → XP_036016913.1 E3 SUMO-protein ligase PIAS2 isoform X4

Conserved Domains (3) summary

smart00513
Location:11 → 45

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

cd16790
Location:343 → 390

SP-RING_PIAS; SP-RING finger found in protein inhibitor of activated signal transducer and activator of transcription (PIAS) proteins

pfam14324
Location:147 → 296

PINIT; PINIT domain
XM_006526425.4 → XP_006526488.1 E3 SUMO-protein ligase PIAS2 isoform X2

Conserved Domains (3) summary

smart00513
Location:4 → 38

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

cd16790
Location:336 → 383

SP-RING_PIAS; SP-RING finger found in protein inhibitor of activated signal transducer and activator of transcription (PIAS) proteins

pfam14324
Location:140 → 284

PINIT; PINIT domain
XM_036161017.1 → XP_036016910.1 E3 SUMO-protein ligase PIAS2 isoform X3

Conserved Domains (3) summary

smart00513
Location:4 → 38

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

cd16790
Location:336 → 383

SP-RING_PIAS; SP-RING finger found in protein inhibitor of activated signal transducer and activator of transcription (PIAS) proteins

pfam14324
Location:140 → 289

PINIT; PINIT domain
XM_036161021.1 → XP_036016914.1 E3 SUMO-protein ligase PIAS2 isoform X5

Conserved Domains (3) summary

smart00513
Location:4 → 38

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

cd16790
Location:336 → 383

SP-RING_PIAS; SP-RING finger found in protein inhibitor of activated signal transducer and activator of transcription (PIAS) proteins

pfam14324
Location:140 → 289

PINIT; PINIT domain
XM_036161023.1 → XP_036016916.1 E3 SUMO-protein ligase PIAS2 isoform X5

Conserved Domains (3) summary

smart00513
Location:4 → 38

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

cd16790
Location:336 → 383

SP-RING_PIAS; SP-RING finger found in protein inhibitor of activated signal transducer and activator of transcription (PIAS) proteins

pfam14324
Location:140 → 289

PINIT; PINIT domain