Mag myelin-associated glycoprotein [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Magprovided by MGI
Official Full Name: myelin-associated glycoproteinprovided by MGI
Primary source: MGI:MGI:96912
See related: Ensembl:ENSMUSG00000036634 AllianceGenome:MGI:96912
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Gma; siglec-4a
Summary: This gene encodes a type I membrane protein and member of the immunoglobulin-like superfamily. It is expressed in myelinating glial cells, including oligodendrocytes of the central nervous system and Schwann cells of the peripheral nervous system. Mice lacking the encoded protein express abundant myelin, but suffer long-term axon degeneration, altered distribution of channels and adhesion molecules at nodes of Ranvier, and altered axon cytoskeletal structure. While not required for myelination, the encoded protein enhances axon-myelin stability, helps to structure nodes of Ranvier, and regulates the axon cytoskeleton. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
Expression: Biased expression in cerebellum adult (RPKM 107.5), cortex adult (RPKM 67.5) and 2 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 B1; 7 19.26 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (30598600..30614347, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (30899175..30914922, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Focused ion beam-scanning electron microscopy links pathological myelin outfoldings to axonal changes in mice lacking Plp1 or Mag.
Title: Focused ion beam-scanning electron microscopy links pathological myelin outfoldings to axonal changes in mice lacking Plp1 or Mag.
The combination of trans ganglioside binding and cis homodimerization explains how MAG maintains the myelin-axon spacing and provides a mechanism for MAG-mediated bi-directional signalling.
Title: Structural basis of myelin-associated glycoprotein adhesion and signalling.
We show that myelin-associated glycoprotein or CNS myelin, in general, inhibit rodent Schwann cell migration and induce their death via cleavage of the neurotrophin receptor p75.
Title: Myelin-Associated Glycoprotein Inhibits Schwann Cell Migration and Induces Their Death.
Results show that MAG is important for axon-glia contact in a model for Charcot-Marie-Tooth disease type 1A, and suggest that its increased expression in patients has a compensatory role in the disease pathology
Title: An essential role of MAG in mediating axon-myelin attachment in Charcot-Marie-Tooth 1A disease.
the apparent dependency on sulfatide for maintaining Nfasc155 and MAG associations is intriguing since the fatty acid composition of sulfatide is altered and paranodal ultrastructure is compromised in multiple sclerosis
Title: Nfasc155H and MAG are specifically susceptible to detergent extraction in the absence of the myelin sphingolipid sulfatide.
LRP1 assembles unique co-receptor systems to initiate cell signaling in response to tissue-type plasminogen activator and myelin-associated glycoprotein.
Title: LRP1 assembles unique co-receptor systems to initiate cell signaling in response to tissue-type plasminogen activator and myelin-associated glycoprotein.
Heteronuclear ribonucleoprotein A1 interacts with an element that overlaps the 5' splice site of Mag exon 12.
Title: hnRNP A1 and secondary structure coordinate alternative splicing of Mag.
Ablation of neuronal ceramide synthase 1 in mice decreases ganglioside levels and expression of myelin-associated glycoprotein in oligodendrocytes.
Title: Ablation of neuronal ceramide synthase 1 in mice decreases ganglioside levels and expression of myelin-associated glycoprotein in oligodendrocytes.
MAG-mediated axon protection against VNC is suppressed in CRMP4-/- DRG neurons.
Title: CRMP4 mediates MAG-induced inhibition of axonal outgrowth and protection against Vincristine-induced axonal degeneration.
myelin-associated glycoprotein (MAG) performs distinct actions on the formation, maturation, degeneration and regeneration of sciatic nerve myelin sheath.
Title: Age-related changes in myelin morphology, electrophysiological property and myelin-associated protein expression of mouse sciatic nerves.

Submit: New GeneRIF Correction See all GeneRIFs (24)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (5) 1 citation
Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Mag (e-PCR), detects polymorphism
- UniSTS:143816

UniSTS:143817 (e-PCR)
- UniSTS:143817

Mag (e-PCR), detects polymorphism
- UniSTS:143817

Mag (e-PCR), detects polymorphism
- UniSTS:495846

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables carbohydrate binding	IEA Inferred from Electronic Annotation more info
enables ganglioside GT1b binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables ganglioside GT1b binding	ISO Inferred from Sequence Orthology more info
enables lipid binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables sialic acid binding	IBA Inferred from Biological aspect of Ancestor more info
enables sialic acid binding	IDA Inferred from Direct Assay more info	PubMed
enables sialic acid binding	ISO Inferred from Sequence Orthology more info
enables signaling receptor binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within axon regeneration	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cell adhesion	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in cell-cell adhesion via plasma-membrane adhesion molecules	ISO Inferred from Sequence Orthology more info
involved_in central nervous system myelin formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in central nervous system myelination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in myelination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in myelination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of axon extension	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of neuron apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of neuron differentiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of neuron projection development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of neuron projection development	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of astrocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of myelination	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within transmission of nerve impulse	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Schmidt-Lanterman incisure	IDA Inferred from Direct Assay more info	PubMed
located_in Schmidt-Lanterman incisure	ISO Inferred from Sequence Orthology more info
located_in compact myelin	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mesaxon	ISO Inferred from Sequence Orthology more info
located_in myelin sheath	HDA more info	PubMed
located_in myelin sheath	IDA Inferred from Direct Assay more info	PubMed
located_in myelin sheath	ISO Inferred from Sequence Orthology more info
located_in myelin sheath adaxonal region	IDA Inferred from Direct Assay more info	PubMed
located_in myelin sheath adaxonal region	ISO Inferred from Sequence Orthology more info
located_in paranode region of axon	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: myelin-associated glycoprotein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001346084.2 → NP_001333013.1 myelin-associated glycoprotein isoform 1 precursor

Status: REVIEWED

Source sequence(s)

AC165340

Consensus CDS

CCDS90207.1

UniProtKB/Swiss-Prot

A0A087WPR1, P16880, P20917

UniProtKB/TrEMBL

Q3ZB61

Related

ENSMUSP00000139881.2, ENSMUST00000191081.7

Conserved Domains (5) summary

smart00409
Location:333 → 409

IG; Immunoglobulin

pfam13927
Location:239 → 309

Ig_3; Immunoglobulin domain

cd00096
Location:239 → 242

Ig; Ig strand A [structural motif]

cd05712
Location:22 → 137

IgV_CD33; Immunoglobulin Variable (IgV) domain at the N-terminus of CD33 and related Siglecs (sialic acid-binding Ig-like lectins)

cd20987
Location:141 → 235

IgC2_CD33_d2_like; Second immunoglobulin domain of Cluster of Differentiation (CD) 33 and related Siglecs; member of the C2-set of IgSF domains
NM_001346085.2 → NP_001333014.1 myelin-associated glycoprotein isoform 1 precursor

Status: REVIEWED

Source sequence(s)

AC165340

Consensus CDS

CCDS90207.1

UniProtKB/Swiss-Prot

A0A087WPR1, P16880, P20917

UniProtKB/TrEMBL

Q3ZB61

Conserved Domains (5) summary

smart00409
Location:333 → 409

IG; Immunoglobulin

pfam13927
Location:239 → 309

Ig_3; Immunoglobulin domain

cd00096
Location:239 → 242

Ig; Ig strand A [structural motif]

cd05712
Location:22 → 137

IgV_CD33; Immunoglobulin Variable (IgV) domain at the N-terminus of CD33 and related Siglecs (sialic acid-binding Ig-like lectins)

cd20987
Location:141 → 235

IgC2_CD33_d2_like; Second immunoglobulin domain of Cluster of Differentiation (CD) 33 and related Siglecs; member of the C2-set of IgSF domains
NM_001346086.2 → NP_001333015.1 myelin-associated glycoprotein isoform 3 precursor

Status: REVIEWED

Description

Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate exon in the 3' coding region resulting in novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (3) has a shorter and distinct C-terminus, compared to isoform 1. Variants 4, 5, 6, and 7 encode the same isoform (3).

Source sequence(s)

AC165340

Consensus CDS

CCDS21115.1

UniProtKB/TrEMBL

Q3T1F6

Conserved Domains (5) summary

smart00409
Location:333 → 409

IG; Immunoglobulin

pfam13927
Location:239 → 309

Ig_3; Immunoglobulin domain

cd00096
Location:239 → 242

Ig; Ig strand A [structural motif]

cd05712
Location:22 → 137

IgV_CD33; Immunoglobulin Variable (IgV) domain at the N-terminus of CD33 and related Siglecs (sialic acid-binding Ig-like lectins)

cd20987
Location:141 → 235

IgC2_CD33_d2_like; Second immunoglobulin domain of Cluster of Differentiation (CD) 33 and related Siglecs; member of the C2-set of IgSF domains
NM_001346087.2 → NP_001333016.1 myelin-associated glycoprotein isoform 3 precursor

Status: REVIEWED

Description

Transcript Variant: This variant (5) differs in the 5' UTR and uses an alternate exon in the 3' coding region resulting in novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (3) has a shorter and distinct C-terminus, compared to isoform 1. Variants 4, 5, 6, and 7 encode the same isoform (3).

Source sequence(s)

AC165340

Consensus CDS

CCDS21115.1

UniProtKB/TrEMBL

Q3T1F6

Conserved Domains (5) summary

smart00409
Location:333 → 409

IG; Immunoglobulin

pfam13927
Location:239 → 309

Ig_3; Immunoglobulin domain

cd00096
Location:239 → 242

Ig; Ig strand A [structural motif]

cd05712
Location:22 → 137

IgV_CD33; Immunoglobulin Variable (IgV) domain at the N-terminus of CD33 and related Siglecs (sialic acid-binding Ig-like lectins)

cd20987
Location:141 → 235

IgC2_CD33_d2_like; Second immunoglobulin domain of Cluster of Differentiation (CD) 33 and related Siglecs; member of the C2-set of IgSF domains
NM_001346088.2 → NP_001333017.1 myelin-associated glycoprotein isoform 3 precursor

Status: REVIEWED

Description

Transcript Variant: This variant (7) differs in the 5' UTR and uses an alternate exon in the 3' coding region resulting in novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (3) has a shorter and distinct C-terminus, compared to isoform 1. Variants 4, 5, 6, and 7 encode the same isoform (3).

Source sequence(s)

AC165340

Consensus CDS

CCDS21115.1

UniProtKB/TrEMBL

Q3T1F6

Conserved Domains (5) summary

smart00409
Location:333 → 409

IG; Immunoglobulin

pfam13927
Location:239 → 309

Ig_3; Immunoglobulin domain

cd00096
Location:239 → 242

Ig; Ig strand A [structural motif]

cd05712
Location:22 → 137

IgV_CD33; Immunoglobulin Variable (IgV) domain at the N-terminus of CD33 and related Siglecs (sialic acid-binding Ig-like lectins)

cd20987
Location:141 → 235

IgC2_CD33_d2_like; Second immunoglobulin domain of Cluster of Differentiation (CD) 33 and related Siglecs; member of the C2-set of IgSF domains
NM_001346089.2 → NP_001333018.1 myelin-associated glycoprotein isoform 2 precursor

Status: REVIEWED

Source sequence(s)

AC165340

UniProtKB/TrEMBL

Q3ZB60

Related

ENSMUSP00000140526.2, ENSMUST00000188569.7
NM_001369262.1 → NP_001356191.1 myelin-associated glycoprotein isoform 1 precursor

Status: REVIEWED

Description

Transcript Variant: This variant (8) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 8, and 9 all encode the same isoform (1).

Source sequence(s)

AC165340

Consensus CDS

CCDS90207.1

UniProtKB/Swiss-Prot

A0A087WPR1, P16880, P20917

UniProtKB/TrEMBL

Q3ZB61

Conserved Domains (5) summary

smart00409
Location:333 → 409

IG; Immunoglobulin

pfam13927
Location:239 → 309

Ig_3; Immunoglobulin domain

cd00096
Location:239 → 242

Ig; Ig strand A [structural motif]

cd05712
Location:22 → 137

IgV_CD33; Immunoglobulin Variable (IgV) domain at the N-terminus of CD33 and related Siglecs (sialic acid-binding Ig-like lectins)

cd20987
Location:141 → 235

IgC2_CD33_d2_like; Second immunoglobulin domain of Cluster of Differentiation (CD) 33 and related Siglecs; member of the C2-set of IgSF domains
NM_001416848.1 → NP_001403777.1 myelin-associated glycoprotein isoform 1 precursor

Status: REVIEWED

Source sequence(s)

AC165340

UniProtKB/Swiss-Prot

A0A087WPR1, P16880, P20917
NM_010758.4 → NP_034888.1 myelin-associated glycoprotein isoform 3 precursor

See identical proteins and their annotated locations for NP_034888.1

Status: REVIEWED

Description

Transcript Variant: This variant (6) uses an alternate exon in the 3' coding region resulting in novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (3) has a shorter and distinct C-terminus, compared to isoform 1. Variants 4, 5, 6, and 7 encode the same isoform (3).

Source sequence(s)

AC165340

Consensus CDS

CCDS21115.1

UniProtKB/TrEMBL

Q3T1F6

Related

ENSMUSP00000139564.2, ENSMUST00000187137.7

Conserved Domains (5) summary

smart00409
Location:333 → 409

IG; Immunoglobulin

pfam13927
Location:239 → 309

Ig_3; Immunoglobulin domain

cd00096
Location:239 → 242

Ig; Ig strand A [structural motif]

cd05712
Location:22 → 137

IgV_CD33; Immunoglobulin Variable (IgV) domain at the N-terminus of CD33 and related Siglecs (sialic acid-binding Ig-like lectins)

cd20987
Location:141 → 235

IgC2_CD33_d2_like; Second immunoglobulin domain of Cluster of Differentiation (CD) 33 and related Siglecs; member of the C2-set of IgSF domains

RNA

NR_184397.1 RNA Sequence

Status: REVIEWED

Source sequence(s)

AC165340

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

30598600..30614347 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011250440.3 → XP_011248742.1 myelin-associated glycoprotein isoform X1

See identical proteins and their annotated locations for XP_011248742.1

UniProtKB/Swiss-Prot

A0A087WPR1, P16880, P20917

UniProtKB/TrEMBL

Q3ZB61

Conserved Domains (5) summary

smart00409
Location:333 → 409

IG; Immunoglobulin

pfam13927
Location:239 → 309

Ig_3; Immunoglobulin domain

cd00096
Location:239 → 242

Ig; Ig strand A [structural motif]

cd05712
Location:22 → 137

IgV_CD33; Immunoglobulin Variable (IgV) domain at the N-terminus of CD33 and related Siglecs (sialic acid-binding Ig-like lectins)

cd20987
Location:141 → 235

IgC2_CD33_d2_like; Second immunoglobulin domain of Cluster of Differentiation (CD) 33 and related Siglecs; member of the C2-set of IgSF domains
XM_030242189.2 → XP_030098049.1 myelin-associated glycoprotein isoform X2

UniProtKB/TrEMBL

Q3T1F6

Conserved Domains (5) summary

smart00409
Location:333 → 409

IG; Immunoglobulin

pfam13927
Location:239 → 309

Ig_3; Immunoglobulin domain

cd00096
Location:239 → 242

Ig; Ig strand A [structural motif]

cd05712
Location:22 → 137

IgV_CD33; Immunoglobulin Variable (IgV) domain at the N-terminus of CD33 and related Siglecs (sialic acid-binding Ig-like lectins)

cd20987
Location:141 → 235

IgC2_CD33_d2_like; Second immunoglobulin domain of Cluster of Differentiation (CD) 33 and related Siglecs; member of the C2-set of IgSF domains
XM_011250439.3 → XP_011248741.1 myelin-associated glycoprotein isoform X1

See identical proteins and their annotated locations for XP_011248741.1

UniProtKB/Swiss-Prot

A0A087WPR1, P16880, P20917

UniProtKB/TrEMBL

Q3ZB61

Conserved Domains (5) summary

smart00409
Location:333 → 409

IG; Immunoglobulin

pfam13927
Location:239 → 309

Ig_3; Immunoglobulin domain

cd00096
Location:239 → 242

Ig; Ig strand A [structural motif]

cd05712
Location:22 → 137

IgV_CD33; Immunoglobulin Variable (IgV) domain at the N-terminus of CD33 and related Siglecs (sialic acid-binding Ig-like lectins)

cd20987
Location:141 → 235

IgC2_CD33_d2_like; Second immunoglobulin domain of Cluster of Differentiation (CD) 33 and related Siglecs; member of the C2-set of IgSF domains
XM_036152725.1 → XP_036008618.1 myelin-associated glycoprotein isoform X1

UniProtKB/Swiss-Prot

A0A087WPR1, P16880, P20917

UniProtKB/TrEMBL

Q3ZB61

Conserved Domains (5) summary

smart00409
Location:333 → 409

IG; Immunoglobulin

pfam13927
Location:239 → 309

Ig_3; Immunoglobulin domain

cd00096
Location:239 → 242

Ig; Ig strand A [structural motif]

cd05712
Location:22 → 137

IgV_CD33; Immunoglobulin Variable (IgV) domain at the N-terminus of CD33 and related Siglecs (sialic acid-binding Ig-like lectins)

cd20987
Location:141 → 235

IgC2_CD33_d2_like; Second immunoglobulin domain of Cluster of Differentiation (CD) 33 and related Siglecs; member of the C2-set of IgSF domains