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Prima1 proline rich membrane anchor 1 [ Mus musculus (house mouse) ]

Gene ID: 170952, updated on 5-Mar-2024

Summary

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Official Symbol
Prima1provided by MGI
Official Full Name
proline rich membrane anchor 1provided by MGI
Primary source
MGI:MGI:1926097
See related
Ensembl:ENSMUSG00000041669 AllianceGenome:MGI:1926097
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
PRiMA; B230212M13Rik
Summary
Enables enzyme binding activity and protein-membrane adaptor activity. Acts upstream of or within establishment of localization in cell. Is intrinsic component of plasma membrane. Orthologous to human PRIMA1 (proline rich membrane anchor 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in stomach adult (RPKM 7.2), bladder adult (RPKM 4.3) and 19 other tissues See more
Orthologs
human all
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Genomic context

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See Prima1 in Genome Data Viewer
Location:
12 E; 12 52.9 cM
Exon count:
7
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 12 NC_000078.7 (103160712..103208967, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 12 NC_000078.6 (103194453..103242719, complement)

Chromosome 12 - NC_000078.7Genomic Context describing neighboring genes Neighboring gene cytochrome c oxidase subunit 8C Neighboring gene unc-79 homolog Neighboring gene STARR-seq mESC enhancer starr_33121 Neighboring gene STARR-positive B cell enhancer ABC_E7173 Neighboring gene CapStarr-seq enhancer MGSCv37_chr12:104569739-104569961 Neighboring gene STARR-positive B cell enhancer ABC_E4038 Neighboring gene family with sequence similarity 181, member A Neighboring gene STARR-positive B cell enhancer ABC_E1863 Neighboring gene predicted gene 29508 Neighboring gene predicted gene 15523 Neighboring gene ankyrin repeat and SOCS box-containing 2

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Schwann cells sense and control acetylcholine spillover at the neuromuscular junction by α7 nicotinic receptors and butyrylcholinesterase. Petrov KA, et al. J Neurosci, 2014 Sep 3. PMID 25186736, Free PMC Article
  2. Developmental adaptation of central nervous system to extremely high acetylcholine levels. Farar V, et al. PLoS One, 2013. PMID 23861875, Free PMC Article
  3. The AChE membrane-binding tail PRiMA is down-regulated in muscle and nerve of mice with muscular dystrophy by merosin deficiency. Vidal CJ, et al. Chem Biol Interact, 2013 Mar 25. PMID 22906800
  4. PRIMA-1MET inhibits growth of mouse tumors carrying mutant p53. Zache N, et al. Cell Oncol, 2008. PMID 18791272, Free PMC Article
  5. Assembly of acetylcholinesterase tetramers by peptidic motifs from the proline-rich membrane anchor, PRiMA: competition between degradation and secretion pathways of heteromeric complexes. Noureddine H, et al. J Biol Chem, 2007 Feb 9. PMID 17158452

See all (31) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. It interacts with PS1 and undergoes proteolytic processing.
    Title: Presenilin-1 influences processing of the acetylcholinesterase membrane anchor PRiMA.
  2. Elevated expression of muscarinic receptors is responsible for physiological adaptation of PRiMA-deficient developing mice.
    Title: Developmental adaptation of central nervous system to extremely high acetylcholine levels.
  3. the relative content of AChE, BuChE and PRiMA mRNAs were compared in normal and Lama2dy muscle and sciatic nerve
    Title: The AChE membrane-binding tail PRiMA is down-regulated in muscle and nerve of mice with muscular dystrophy by merosin deficiency.
  4. The N-linked glycosylation of AChE(T) plays a major role for acquisition of AChE full enzymatic activity but does not affect its oligomerization.
    Title: The assembly of proline-rich membrane anchor (PRiMA)-linked acetylcholinesterase enzyme: glycosylation is required for enzymatic activity but not for oligomerization.
  5. Data show that along the nerve terminus the vast majority of acetylcholinesterase is anchored by collagen Q that is only produced by the muscle, whereas very minor amounts of AChE are anchored by PRiMA that is produced by motoneurons.
    Title: Distinct localization of collagen Q and PRiMA forms of acetylcholinesterase at the neuromuscular junction.
  6. Proline-rich membrane anchor protein is necessary to target and/or to stabilize nascent acetylcholinesterase in neurons.
    Title: Targeting of acetylcholinesterase in neurons in vivo: a dual processing function for the proline-rich membrane anchor subunit and the attachment domain on the catalytic subunit.
  7. PRIMA-1MET targets mouse tumors carrying mutant p53
    Title: PRIMA-1MET inhibits growth of mouse tumors carrying mutant p53.
  8. Acetylcholinesterase associates differently with its anchoring proteins ColQ and PRiMA
    Title: Acetylcholinesterase associates differently with its anchoring proteins ColQ and PRiMA.
  9. PRiMA expression is predominantly to the cholinergic system and that anchoring of cholinesterases to cell membranes by PRiMA represents a limiting factor for production of the AChE tailed splice variant (AChET)-PRiMA complex.
    Title: Expression of PRiMA in the mouse brain: membrane anchoring and accumulation of 'tailed' acetylcholinesterase.
  10. expression of PRiMA, as well as PRiMA-associated G(4) AChE, in muscle is suppressed by muscle regulatory factors, muscular activity, and nerve-derived trophic factors
    Title: Regulation of a transcript encoding the proline-rich membrane anchor of globular muscle acetylcholinesterase. The suppressive roles of myogenesis and innervating nerves.
Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (1)  1 citation
  • Endonuclease-mediated (1) 

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables enzyme binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein-membrane adaptor activity IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within establishment of localization in cell IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in anchoring junction IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in synapse IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
proline-rich membrane anchor 1
Names
acetylcholinesterase membrane anchor

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_133364.2 → NP_579942.2  proline-rich membrane anchor 1 precursor

    See identical proteins and their annotated locations for NP_579942.2

    Status: VALIDATED

    Source sequence(s)
    AK039705, AK140034, AY043275
    Consensus CDS
    CCDS26127.1
    UniProtKB/Swiss-Prot
    Q810F0, Q8VHC4
    UniProtKB/TrEMBL
    F8VQJ2
    Related
    ENSMUSP00000074017.4, ENSMUST00000074416.10
    Conserved Domains (1) summary
    pfam16101
    Location:37 → 153
    PRIMA1; Proline-rich membrane anchor 1

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000078.7 Reference GRCm39 C57BL/6J

    Range
    103160712..103208967 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006515507.5 → XP_006515570.1  proline-rich membrane anchor 1 isoform X1

    See identical proteins and their annotated locations for XP_006515570.1

    UniProtKB/Swiss-Prot
    Q810F0, Q8VHC4
    UniProtKB/TrEMBL
    F8VQJ2
    Conserved Domains (1) summary
    pfam16101
    Location:37 → 153
    PRIMA1; Proline-rich membrane anchor 1

  2. XM_006515506.3 → XP_006515569.1  proline-rich membrane anchor 1 isoform X1

    See identical proteins and their annotated locations for XP_006515569.1

    UniProtKB/Swiss-Prot
    Q810F0, Q8VHC4
    UniProtKB/TrEMBL
    F8VQJ2
    Conserved Domains (1) summary
    pfam16101
    Location:37 → 153
    PRIMA1; Proline-rich membrane anchor 1

RNA

  1. XR_381482.2 RNA Sequence

  2. XR_381481.2 RNA Sequence

    Related
    ENSMUST00000127843.8

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_178023.2: Suppressed sequence

    Description
    NM_178023.2: This RefSeq was permanently suppressed because it is a nonsense-mediated mRNA decay (NMD) candidate.
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q810F0.2 GenPept UniProtKB/Swiss-Prot:Q810F0

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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