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Klf3 Kruppel-like transcription factor 3 (basic) [ Mus musculus (house mouse) ]

Gene ID: 16599, updated on 21-Apr-2024

Summary

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Official Symbol
Klf3provided by MGI
Official Full Name
Kruppel-like transcription factor 3 (basic)provided by MGI
Primary source
MGI:MGI:1342773
See related
Ensembl:ENSMUSG00000029178 AllianceGenome:MGI:1342773
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Bklf; Tef-2; 9930027G08Rik
Summary
Enables RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Involved in negative regulation of transcription by RNA polymerase II. Predicted to be located in nucleoplasm. Is expressed in several structures, including alimentary system; brain; early conceptus; genitourinary system; and integumental system. Orthologous to human KLF3 (Kruppel like factor 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in colon adult (RPKM 33.8), thymus adult (RPKM 20.9) and 27 other tissues See more
Orthologs
human all
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Genomic context

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Location:
5 C3.1; 5 33.49 cM
Exon count:
8
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 5 NC_000071.7 (64960864..64990246)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 5 NC_000071.6 (64803521..64832901)

Chromosome 5 - NC_000071.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_13223 Neighboring gene predicted gene, 54285 Neighboring gene STARR-positive B cell enhancer mm9_chr5:65053140-65053441 Neighboring gene STARR-positive B cell enhancer mm9_chr5:65053800-65054101 Neighboring gene STARR-seq mESC enhancer starr_13227 Neighboring gene STARR-seq mESC enhancer starr_13228 Neighboring gene uncharacterized LOC105244755 Neighboring gene STARR-seq mESC enhancer starr_13234 Neighboring gene STARR-seq mESC enhancer starr_13235 Neighboring gene CapStarr-seq enhancer MGSCv37_chr5:65155737-65155920 Neighboring gene STARR-positive B cell enhancer ABC_E10322 Neighboring gene CapStarr-seq enhancer MGSCv37_chr5:65197351-65197625 Neighboring gene predicted gene, 54286 Neighboring gene STARR-seq mESC enhancer starr_13242 Neighboring gene predicted gene, 31684 Neighboring gene STARR-seq mESC enhancer starr_13247 Neighboring gene predicted gene, 57736 Neighboring gene VISTA enhancer mm1725 Neighboring gene STARR-positive B cell enhancer ABC_E8057 Neighboring gene STARR-positive B cell enhancer ABC_E8058 Neighboring gene predicted gene, 31889

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells. Hao J, et al. Cell Prolif, 2020 Nov. PMID 32990380, Free PMC Article
  2. Eosinophil function in adipose tissue is regulated by Krüppel-like factor 3 (KLF3). Knights AJ, et al. Nat Commun, 2020 Jun 10. PMID 32523103, Free PMC Article
  3. Regions outside the DNA-binding domain are critical for proper in vivo specificity of an archetypal zinc finger transcription factor. Burdach J, et al. Nucleic Acids Res, 2014 Jan. PMID 24106088, Free PMC Article
  4. Proviral integration of an Abelson-murine leukemia virus deregulates BKLF-expression in the hypermutating pre-B cell line 18-81. Kirberg J, et al. Mol Immunol, 2005 Jun. PMID 15829312
  5. Isolation and characterization of the cDNA encoding BKLF/TEF-2, a major CACCC-box-binding protein in erythroid cells and selected other cells. Crossley M, et al. Mol Cell Biol, 1996 Apr. PMID 8657145, Free PMC Article

See all (64) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. KLF3 Transcription Activates WNT1 and Promotes the Growth and Metastasis of Gastric Cancer via Activation of the WNT/beta-Catenin Signaling Pathway.
    Title: KLF3 Transcription Activates WNT1 and Promotes the Growth and Metastasis of Gastric Cancer via Activation of the WNT/β-Catenin Signaling Pathway.
  2. miR-324-5p promotes adipocyte differentiation and lipid droplet accumulation by targeting Krueppel-like factor 3 (KLF3).
    Title: miR-324-5p promotes adipocyte differentiation and lipid droplet accumulation by targeting Krueppel-like factor 3 (KLF3).
  3. Kruppel-like factor 3 (KLF3) suppresses NF-kappaB-driven inflammation in mice.
    Title: Krüppel-like factor 3 (KLF3) suppresses NF-κB-driven inflammation in mice.
  4. KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.
    Title: KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.
  5. miR-20a-5p promotes adipocyte differentiation from bone marrow-derived stromal cells by targeting and negatively regulating Klf3 in the early phase during the procedure of adipogenesis.
    Title: miR-20a-5p promotes adipogenic differentiation of murine bone marrow stromal cells via targeting Kruppel-like factor 3.
  6. KLF1 and KLF3 bind common as well as unique sites within the erythroid cell genome.
    Title: Krüppel-like factors compete for promoters and enhancers to fine-tune transcription.
  7. mechanistic insights into the regulation of Lgals3, demonstrating that C-terminal binding protein (CtBP) is required to drive optimal KLF3-mediated silencing.
    Title: Krüppel-like Factor 3 (KLF3/BKLF) Is Required for Widespread Repression of the Inflammatory Modulator Galectin-3 (Lgals3).
  8. Cineromycin B inhibited adipocyte differentiation in 3T3-L1 cells mainly via upregulation of KLF2 and KLF3 mRNA expression at an early stage of the differentiation.
    Title: Cineromycin B isolated from Streptomyces cinerochromogenes inhibits adipocyte differentiation of 3T3-L1 cells via Krüppel-like factors 2 and 3.
  9. Phosphorylation of KLF3 and CtBP2 by HIPK2 strengthens the interaction between these two factors and increases transcriptional repression by KLF3.
    Title: Phosphorylation of Krüppel-like factor 3 (KLF3/BKLF) and C-terminal binding protein 2 (CtBP2) by homeodomain-interacting protein kinase 2 (HIPK2) modulates KLF3 DNA binding and activity.
  10. KLF3 acts as transcriptional repressor for the leukocyte-specific integrin beta7 (Itgb7, Ly69) by binding to the beta7 promoter, as revealed by chromatin immunoprecipitation.
    Title: Leukocyte β7 integrin targeted by Krüppel-like factors.
Submit: New GeneRIF Correction See all GeneRIFs (23)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Gene trapped (2) 
  • Targeted (2)  1 citation
  • Chemically induced (ENU) (1)  1 citation
  • Endonuclease-mediated (2) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC151315

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables DNA binding ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
enables DNA-binding transcription factor activity, RNA polymerase II-specific IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables sequence-specific double-stranded DNA binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in negative regulation of transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Krueppel-like factor 3
Names
CACCC-box-binding protein BKLF
Kruppel-like factor 3 (basic)
basic krueppel-like factor

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_008453.6 → NP_032479.1  Krueppel-like factor 3

    See identical proteins and their annotated locations for NP_032479.1

    Status: VALIDATED

    Source sequence(s)
    AC130535
    Consensus CDS
    CCDS19301.1
    UniProtKB/Swiss-Prot
    Q60980
    UniProtKB/TrEMBL
    Q545J5
    Related
    ENSMUSP00000129363.2, ENSMUST00000165536.8
    Conserved Domains (4) summary
    sd00017
    Location:261 → 283
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:259 → 283
    zf-C2H2; Zinc finger, C2H2 type
    pfam13465
    Location:305 → 330
    zf-H2C2_2; Zinc-finger double domain
    cd21577
    Location:49 → 259
    KLF3_N; N-terminal domain of Kruppel-like factor 3

RNA

  1. NR_165035.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC130535

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000071.7 Reference GRCm39 C57BL/6J

    Range
    64960864..64990246
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006503751.5 → XP_006503814.1  Krueppel-like factor 3 isoform X1

    See identical proteins and their annotated locations for XP_006503814.1

    UniProtKB/Swiss-Prot
    Q60980
    UniProtKB/TrEMBL
    Q545J5
    Conserved Domains (4) summary
    sd00017
    Location:261 → 283
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:259 → 283
    zf-C2H2; Zinc finger, C2H2 type
    pfam13465
    Location:305 → 330
    zf-H2C2_2; Zinc-finger double domain
    cd21577
    Location:49 → 259
    KLF3_N; N-terminal domain of Kruppel-like factor 3
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q60980.1 GenPept UniProtKB/Swiss-Prot:Q60980

Gene LinkOut

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