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Dnaja1 DnaJ heat shock protein family (Hsp40) member A1 [ Mus musculus (house mouse) ]

Gene ID: 15502, updated on 11-Apr-2024

Summary

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Official Symbol
Dnaja1provided by MGI
Official Full Name
DnaJ heat shock protein family (Hsp40) member A1provided by MGI
Primary source
MGI:MGI:1270129
See related
Ensembl:ENSMUSG00000028410 AllianceGenome:MGI:1270129
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Hsj2; HSJ-2; Nedd7
Summary
The protein encoded by this gene is a member of the DnaJ family, whose members act as cochaperones of heat shock protein 70. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. Mice deficient for this gene display reduced levels of activation‐induced deaminase, an enzyme that deaminates deoxycytidine at the immunoglobulin genes during immune responses. In addition, mice lacking this gene exhibit severe defects in spermatogenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Expression
Broad expression in CNS E11.5 (RPKM 48.9), CNS E14 (RPKM 41.5) and 19 other tissues See more
Orthologs
human all
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Genomic context

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See Dnaja1 in Genome Data Viewer
Location:
4 A5; 4 20.46 cM
Exon count:
10
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 4 NC_000070.7 (40722468..40734965)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 4 NC_000070.6 (40722468..40734965)

Chromosome 4 - NC_000070.7Genomic Context describing neighboring genes Neighboring gene RIKEN cDNA 4933428C19 gene Neighboring gene STARR-seq mESC enhancer starr_09986 Neighboring gene STARR-seq mESC enhancer starr_09987 Neighboring gene STARR-positive B cell enhancer ABC_E2688 Neighboring gene aprataxin Neighboring gene STARR-positive B cell enhancer ABC_E1272 Neighboring gene predicted gene 6297 Neighboring gene microRNA 207 Neighboring gene STARR-positive B cell enhancer ABC_E7962 Neighboring gene smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans) Neighboring gene STARR-positive B cell enhancer ABC_E4617 Neighboring gene presenilin enhancer gamma secretase subunit, pseudogene Neighboring gene predicted gene, 34213

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Modulation of chaperone activities of Hsp70 and Hsp70-2 by a mammalian DnaJ/Hsp40 homolog, DjA4. Hafizur RM, et al. J Biochem, 2004 Feb. PMID 15047721
  2. hsp70-DnaJ chaperone pairs prevent nitric oxide-mediated apoptosis in RAW 264.7 macrophages. Gotoh T, et al. Cell Death Differ, 2001 Apr. PMID 11550087
  3. The human DnaJ homologue dj2 facilitates mitochondrial protein import and luciferase refolding. Terada K, et al. J Cell Biol, 1997 Dec 1. PMID 9382858, Free PMC Article
  4. Multiple molecules of Hsc70 and a dimer of DjA1 independently bind to an unfolded protein. Terada K, et al. J Biol Chem, 2010 May 28. PMID 20363747, Free PMC Article
  5. Early defect in the expression of mouse sperm DNAJ 1, a member of the DNAJ/heat shock protein 40 chaperone protein family, in the spinal cord of the wobbler mouse, a murine model of motoneuronal degeneration. Boillée S, et al. Neuroscience, 2002. PMID 12182889

See all (66) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Cereblon Regulates the Proteotoxicity of Tau by Tuning the Chaperone Activity of DNAJA1.
    Title: Cereblon Regulates the Proteotoxicity of Tau by Tuning the Chaperone Activity of DNAJA1.
  2. Identification of a druggable protein-protein interaction site between mutant p53 and its stabilizing chaperone DNAJA1.
    Title: Identification of a druggable protein-protein interaction site between mutant p53 and its stabilizing chaperone DNAJA1.
  3. Our results show that farnesylated DNAJA1 is a crucial chaperone in maintaining mutant p53 stabilization and targeting farnesylated DNAJA1 by atorvastatin will be critical for inhibiting p53 mutant cancer.
    Title: Inhibition of mutant Kras and p53-driven pancreatic carcinogenesis by atorvastatin: Mainly via targeting of the farnesylated DNAJA1 in chaperoning mutant p53.
  4. DnaJa1 farnesylation is required to maintain, and farnesyltransferase inhibition reduces, AID protein levels in B cells. Thus, DnaJa1 is a limiting factor that plays a non-redundant role in the functional stabilization of AID
    Title: Optimal functional levels of activation-induced deaminase specifically require the Hsp40 DnaJa1.
  5. Hsc70 and a dimer of DjA1 independently bind to an unfolded protein
    Title: Multiple molecules of Hsc70 and a dimer of DjA1 independently bind to an unfolded protein.
  6. HSP40 binding is the first step in the HSP90 chaperoning pathway for the progesterone receptor
    Title: HSP40 binding is the first step in the HSP90 chaperoning pathway for the progesterone receptor.
  7. Results indicate that DjA1 and DjB4 of subfamilies A and B of human Hsp40 have different quaternary structures and chaperone functions.
    Title: Low resolution structural study of two human HSP40 chaperones in solution. DJA1 from subfamily A and DJB4 from subfamily B have different quaternary structures.
  8. DnaJ 1 protein expression is down regulated in the spinal cord and testis of wobbler mouse and precedes motor neuron degeneration.
    Title: Early defect in the expression of mouse sperm DNAJ 1, a member of the DNAJ/heat shock protein 40 chaperone protein family, in the spinal cord of the wobbler mouse, a murine model of motoneuronal degeneration.
  9. loss of DjA1 in led to severe defects in spermatogenesis that involve aberrant androgen signaling
    Title: A type I DnaJ homolog, DjA1, regulates androgen receptor signaling and spermatogenesis.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (3)  1 citation
  • Endonuclease-mediated (2) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATPase activator activity ISO
Inferred from Sequence Orthology
more info
  
enables C3HC4-type RING finger domain binding ISO
Inferred from Sequence Orthology
more info
  
enables G protein-coupled receptor binding ISO
Inferred from Sequence Orthology
more info
  
enables Hsp70 protein binding ISO
Inferred from Sequence Orthology
more info
  
enables Tat protein binding ISO
Inferred from Sequence Orthology
more info
  
enables heat shock protein binding IEA
Inferred from Electronic Annotation
more info
  
enables low-density lipoprotein particle receptor binding ISO
Inferred from Sequence Orthology
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein-folding chaperone binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein-folding chaperone binding ISO
Inferred from Sequence Orthology
more info
  
enables ubiquitin protein ligase binding ISO
Inferred from Sequence Orthology
more info
  
enables unfolded protein binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within androgen receptor signaling pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within flagellated sperm motility IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of JUN kinase activity ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of apoptotic process ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of establishment of protein localization to mitochondrion ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of protein ubiquitination ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of apoptotic process ISO
Inferred from Sequence Orthology
more info
  
involved_in protein folding IEA
Inferred from Electronic Annotation
more info
  
involved_in protein localization to mitochondrion ISO
Inferred from Sequence Orthology
more info
  
involved_in protein refolding IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of protein transport ISO
Inferred from Sequence Orthology
more info
  
involved_in response to heat IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within spermatogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in endoplasmic reticulum IEA
Inferred from Electronic Annotation
more info
  
located_in intracellular membrane-bounded organelle IEA
Inferred from Electronic Annotation
more info
  
located_in membrane ISO
Inferred from Sequence Orthology
more info
  
located_in microtubule cytoskeleton ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrion IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
dnaJ homolog subfamily A member 1
Names
DnaJ (Hsp40) homolog, subfamily A, member 1
dnaJ protein homolog 2
heat shock 40 kDa protein 4
heat shock protein J2
heat shock protein, Dnaj-like 2
neural precursor cell expressed, developmentally down-regulated gene 7

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001164671.2 → NP_001158143.1  dnaJ homolog subfamily A member 1

    See identical proteins and their annotated locations for NP_001158143.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.
    Source sequence(s)
    AK083046, AK145963, BP766105, BY245852, CN669546
    Consensus CDS
    CCDS18049.1
    UniProtKB/Swiss-Prot
    P54102, P63037
    UniProtKB/TrEMBL
    Q3TK61, Q5NTY0
    Related
    ENSMUSP00000129730.2, ENSMUST00000164233.8
    Conserved Domains (1) summary
    PTZ00037
    Location:2 → 394
    PTZ00037; DnaJ_C chaperone protein; Provisional

  2. NM_001164672.2 → NP_001158144.1  dnaJ homolog subfamily A member 1

    See identical proteins and their annotated locations for NP_001158144.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.
    Source sequence(s)
    AK083046, AK145963, BP766105, CK617936
    Consensus CDS
    CCDS18049.1
    UniProtKB/Swiss-Prot
    P54102, P63037
    UniProtKB/TrEMBL
    Q3TK61, Q5NTY0
    Conserved Domains (1) summary
    PTZ00037
    Location:2 → 394
    PTZ00037; DnaJ_C chaperone protein; Provisional

  3. NM_008298.6 → NP_032324.1  dnaJ homolog subfamily A member 1

    See identical proteins and their annotated locations for NP_032324.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.
    Source sequence(s)
    AK083046, AK145963, BB574002, BP766105
    Consensus CDS
    CCDS18049.1
    UniProtKB/Swiss-Prot
    P54102, P63037
    UniProtKB/TrEMBL
    Q3TK61, Q5NTY0
    Related
    ENSMUSP00000030118.4, ENSMUST00000030118.10
    Conserved Domains (1) summary
    PTZ00037
    Location:2 → 394
    PTZ00037; DnaJ_C chaperone protein; Provisional

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000070.7 Reference GRCm39 C57BL/6J

    Range
    40722468..40734965
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P63037.1 GenPept UniProtKB/Swiss-Prot:P63037

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