Grb10 growth factor receptor bound protein 10 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Grb10provided by MGI
Official Full Name: growth factor receptor bound protein 10provided by MGI
Primary source: MGI:MGI:103232
See related: Ensembl:ENSMUSG00000020176 AllianceGenome:MGI:103232
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Meg1; mKIAA0207; 5730571D09Rik
Summary: Enables insulin receptor binding activity and signaling receptor complex adaptor activity. Involved in several processes, including negative regulation of glucose import; negative regulation of signal transduction; and positive regulation of cold-induced thermogenesis. Acts upstream of or within with a negative effect on ERK1 and ERK2 cascade and gene expression. Acts upstream of or within insulin-like growth factor receptor signaling pathway and vascular associated smooth muscle cell migration. Predicted to be located in cytosol. Predicted to be part of protein-containing complex. Is expressed in several structures, including branchial arch; extraembryonic component; genitourinary system; sensory organ; and ventricular layer. Orthologous to human GRB10 (growth factor receptor bound protein 10). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in limb E14.5 (RPKM 223.2), liver E18 (RPKM 212.5) and 13 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 A1; 11 7.15 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (11880499..11987428, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (11930499..12037428, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Hypothalamic Grb10 enhances leptin signalling and promotes weight loss.
Title: Hypothalamic Grb10 enhances leptin signalling and promotes weight loss.
GRB10 regulates beta-cell mass by inhibiting beta-cell proliferation and stimulating beta-cell dedifferentiation.
Title: GRB10 regulates β-cell mass by inhibiting β-cell proliferation and stimulating β-cell dedifferentiation.
Mice lacking paternal expression of imprinted Grb10 are risk-takers.
Title: Mice lacking paternal expression of imprinted Grb10 are risk-takers.
Detailed analysis of paternal knockout Grb10 mice suggests effects on stability of social behavior, rather than social dominance.
Title: Detailed analysis of paternal knockout Grb10 mice suggests effects on stability of social behavior, rather than social dominance.
Grb10 gene could be reactivated in adult mouse liver by acute endoplasmic reticulum stress.
Title: De-silencing Grb10 contributes to acute ER stress-induced steatosis in mouse liver.
ablation of growth factor receptor bound protein 10 (Grb10) in muscle is sufficient to affect muscle size and metabolism, supporting an important role for this protein in growth and metabolic pathways
Title: Ablation of Grb10 Specifically in Muscle Impacts Muscle Size and Glucose Metabolism in Mice.
DNA methylation at CpG island 1 (CGI1) of Grb10 in blastocysts were also significantly decreased after vitrification
Title: Effect of vitrification on in vitro development and imprinted gene Grb10 in mouse embryos.
This study reveals a function for the imprinted gene Grb10 in regulating hematopoietic stem cell self-renewal.
Title: Deletion of the Imprinted Gene Grb10 Promotes Hematopoietic Stem Cell Self-Renewal and Regeneration.
In tumors, Grb10 loss independently promotes Ras pathway hyperactivation, which promotes hyperproliferation, an early feature of tumor development.
Title: Monoallelic loss of the imprinted gene Grb10 promotes tumor formation in irradiated Nf1+/- mice.
This study identified Grb10 as a critical regulator of lipid metabolism, the programming of the browning phenotype, and thermogenesis in adipose tissues.
Title: Grb10 promotes lipolysis and thermogenesis by phosphorylation-dependent feedback inhibition of mTORC1.

Submit: New GeneRIF Correction See all GeneRIFs (41)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (2) 1 citation
Targeted (5) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Meg1 (e-PCR), detects polymorphism
- UniSTS:178187

RH11858 (e-PCR)
- UniSTS:76596

Grb10 (e-PCR), detects polymorphism
- UniSTS:277882

Grb10 (e-PCR), detects polymorphism
- UniSTS:277883

Grb10 (e-PCR), detects polymorphism
- UniSTS:305533

Grb10 (e-PCR), detects polymorphism
- UniSTS:305534

Grb10 (e-PCR), detects polymorphism
- UniSTS:143248

Grb10 (e-PCR), detects polymorphism
- UniSTS:143249

RH136095 (e-PCR)
- UniSTS:210090

AI325020 (e-PCR)
- UniSTS:180156

PMC166222P1 (e-PCR)
- UniSTS:271578

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables insulin receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables insulin receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables insulin receptor binding	ISO Inferred from Sequence Orthology more info
enables phosphotyrosine residue binding	TAS Traceable Author Statement more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-macromolecule adaptor activity	IDA Inferred from Direct Assay more info	PubMed
enables signaling receptor complex adaptor activity	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within_negative_effect ERK1 and ERK2 cascade	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within_negative_effect gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in insulin receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in insulin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within insulin-like growth factor receptor signaling pathway	IPI Inferred from Physical Interaction more info	PubMed
involved_in negative regulation of Wnt signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of Wnt signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of Wnt signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of glucose import	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of glycogen biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of glycogen biosynthetic process	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of insulin receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of insulin receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of cold-induced thermogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of vascular endothelial growth factor receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in response to insulin	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within signal transduction	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within vascular associated smooth muscle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	TAS Traceable Author Statement more info	PubMed
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: growth factor receptor-bound protein 10

Names: GRB10 adapter protein; maternally expressed gene 1 protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001177629.1 → NP_001171100.1 growth factor receptor-bound protein 10 isoform 2

See identical proteins and their annotated locations for NP_001171100.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) contains an alternate 5' non-coding exon, and is missing in-frame coding exons compared to variant 3. This results in a shorter isoform (2) lacking an internal protein segment compared to isoform 3.

Source sequence(s)

AL663087, BC053842, BY122902, CK635114

Consensus CDS

CCDS48754.1

UniProtKB/TrEMBL

Q5SUW3

Related

ENSMUSP00000105281.2, ENSMUST00000109654.8

Conserved Domains (4) summary

cd10415
Location:434 → 541

SH2_Grb10; Src homology 2 (SH2) domain found in the growth factor receptor bound, subclass 10 (Grb10) proteins

cd01259
Location:234 → 356

PH_APBB1IP; Amyloid beta (A4) Precursor protein-Binding, family B, member 1 Interacting Protein pleckstrin homology (PH) domain

cd16141
Location:114 → 205

RA_GRB10; Ras-associating (RA) domain found in growth factor receptor-bound (Grb) protein 10

pfam08947
Location:373 → 414

BPS; BPS (Between PH and SH2)
NM_001370603.1 → NP_001357532.1 growth factor receptor-bound protein 10 isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) represents the longest transcript and encodes isoform (3).

Source sequence(s)

AL645803, AL663087

UniProtKB/Swiss-Prot

O35352, Q3TQ71, Q60760, Q7TSA4, Q8BSH4, Q8BSS5, Q91WC5

UniProtKB/TrEMBL

Q5SUW3

Conserved Domains (4) summary

cd10415
Location:514 → 621

SH2_Grb10; Src homology 2 (SH2) domain found in the growth factor receptor bound, subclass 10 (Grb10) proteins

cd01259
Location:314 → 436

PH_APBB1IP; Amyloid beta (A4) Precursor protein-Binding, family B, member 1 Interacting Protein pleckstrin homology (PH) domain

pfam08947
Location:453 → 494

BPS; BPS (Between PH and SH2)

cd16141
Location:197 → 285

RA_GRB10; Ras-associating (RA) domain found in growth factor receptor-bound (Grb) protein 10
NM_010345.4 → NP_034475.2 growth factor receptor-bound protein 10 isoform 1

See identical proteins and their annotated locations for NP_034475.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) includes an alternate internal exon, compared to variant 3. It encodes isoform 1 which is shorter, compared to isoform 3.

Source sequence(s)

BC053842, CK635114, FJ425892

Consensus CDS

CCDS24440.1

UniProtKB/TrEMBL

Q5SUW3

Related

ENSMUSP00000091011.6, ENSMUST00000093321.12

Conserved Domains (5) summary

cd01787
Location:170 → 251

GRB7_RA; RA (RAS-associated like) domain of Grb7

cd10415
Location:489 → 596

SH2_Grb10; Src homology 2 (SH2) domain found in the growth factor receptor bound, subclass 10 (Grb10) proteins

cd01259
Location:289 → 411

PH_APBB1IP; Amyloid beta (A4) Precursor protein-Binding, family B, member 1 Interacting Protein pleckstrin homology (PH) domain

pfam00169
Location:294 → 402

PH; PH domain

pfam08947
Location:428 → 469

BPS; BPS (Between PH and SH2)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

11880499..11987428 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011243664.2 → XP_011241966.1 growth factor receptor-bound protein 10 isoform X1

See identical proteins and their annotated locations for XP_011241966.1

UniProtKB/TrEMBL

Q5SUW3

Conserved Domains (4) summary

cd10415
Location:434 → 541

SH2_Grb10; Src homology 2 (SH2) domain found in the growth factor receptor bound, subclass 10 (Grb10) proteins

cd01259
Location:234 → 356

PH_APBB1IP; Amyloid beta (A4) Precursor protein-Binding, family B, member 1 Interacting Protein pleckstrin homology (PH) domain

cd16141
Location:114 → 205

RA_GRB10; Ras-associating (RA) domain found in growth factor receptor-bound (Grb) protein 10

pfam08947
Location:373 → 414

BPS; BPS (Between PH and SH2)