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Gapdhs glyceraldehyde-3-phosphate dehydrogenase, spermatogenic [ Mus musculus (house mouse) ]

Gene ID: 14447, updated on 5-Mar-2024

Summary

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Official Symbol
Gapdhsprovided by MGI
Official Full Name
glyceraldehyde-3-phosphate dehydrogenase, spermatogenicprovided by MGI
Primary source
MGI:MGI:95653
See related
Ensembl:ENSMUSG00000061099 AllianceGenome:MGI:95653
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Gapds; Gapd-s
Summary
Enables glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity. Involved in flagellated sperm motility. Acts upstream of or within glycolytic process. Located in sperm fibrous sheath and sperm principal piece. Human ortholog(s) of this gene implicated in Alzheimer's disease. Orthologous to human GAPDHS (glyceraldehyde-3-phosphate dehydrogenase, spermatogenic). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Restricted expression toward testis adult (RPKM 682.7) See more
Orthologs
human all
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Genomic context

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See Gapdhs in Genome Data Viewer
Location:
7 B1; 7 19.23 cM
Exon count:
12
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 7 NC_000073.7 (30429204..30443106, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 7 NC_000073.6 (30729779..30744791, complement)

Chromosome 7 - NC_000073.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E9108 Neighboring gene ATPase, H+/K+ exchanging, gastric, alpha polypeptide Neighboring gene STARR-positive B cell enhancer ABC_E11349 Neighboring gene transmembrane protein 147 Neighboring gene suprabasin Neighboring gene dermokine Neighboring gene keratinocyte differentiation associated protein

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Genomic organization of a mouse glyceraldehyde 3-phosphate dehydrogenase gene (Gapd-s) expressed in post-meiotic spermatogenic cells. Welch JE, et al. Dev Genet, 1995. PMID 7736666
  2. Sperm function, protein phosphorylation, and metabolism differ in mice lacking successive sperm-specific glycolytic enzymes. Huang Z, et al. Biol Reprod, 2017 Oct 1. PMID 29025010
  3. Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility. Miki K, et al. Proc Natl Acad Sci U S A, 2004 Nov 23. PMID 15546993, Free PMC Article
  4. Mouse testis brain RNA-binding protein/translin selectively binds to the messenger RNA of the fibrous sheath protein glyceraldehyde 3-phosphate dehydrogenase-S and suppresses its translation in vitro. Yang J, et al. Biol Reprod, 2003 Mar. PMID 12604635
  5. Glyceraldehyde 3-phosphate dehydrogenase-S protein distribution during mouse spermatogenesis. Bunch DO, et al. Biol Reprod, 1998 Mar. PMID 9510974

See all (32) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. These results identify multiple signaling and metabolic defects that are likely contributors to male infertility and the absence of progressive sperm motility seen in mice lacking GAPDHS.
    Title: Sperm function, protein phosphorylation, and metabolism differ in mice lacking successive sperm-specific glycolytic enzymes.
  2. Gapds(-/-) males were infertile and had profound defects in sperm motility, exhibiting sluggish movement without forward progression.
    Title: Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility.
  3. TB-RBP is involved in the posttranscriptional regulation of Gapds gene expression during spermiogenesis.
    Title: Mouse testis brain RNA-binding protein/translin selectively binds to the messenger RNA of the fibrous sheath protein glyceraldehyde 3-phosphate dehydrogenase-S and suppresses its translation in vitro.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables NAD binding IEA
Inferred from Electronic Annotation
more info
  
enables NADP binding IEA
Inferred from Electronic Annotation
more info
  
enables glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity TAS
Traceable Author Statement
more info
 PubMed 
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in flagellated sperm motility IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within flagellated sperm motility IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in glucose metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in glycolytic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within glycolytic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of glycolytic process TAS
Traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
located_in cilium IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in motile cilium IDA
Inferred from Direct Assay
more info
 PubMed 
located_in motile cilium ISO
Inferred from Sequence Orthology
more info
  
located_in sperm fibrous sheath IDA
Inferred from Direct Assay
more info
 PubMed 
located_in sperm principal piece IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
glyceraldehyde-3-phosphate dehydrogenase, testis-specific
Names
GAPDH-2
spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2
spermatogenic glyceraldehyde-3-phosphate dehydrogenase
NP_001277560.1
NP_032111.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001290631.1 → NP_001277560.1  glyceraldehyde-3-phosphate dehydrogenase, testis-specific isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the longer isoform (1).
    Source sequence(s)
    AC167978
    Consensus CDS
    CCDS71933.1
    UniProtKB/Swiss-Prot
    Q60650, Q64467
    UniProtKB/TrEMBL
    S4R2G5
    Related
    ENSMUSP00000138634.2, ENSMUST00000182634.8
    Conserved Domains (3) summary
    COG0057
    Location:110 → 440
    GapA; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase [Carbohydrate transport and metabolism]
    pfam00044
    Location:110 → 256
    Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain
    pfam02800
    Location:261 → 418
    Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain

  2. NM_008085.2 → NP_032111.1  glyceraldehyde-3-phosphate dehydrogenase, testis-specific isoform 2

    See identical proteins and their annotated locations for NP_032111.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) contains an alternate first exon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.
    Source sequence(s)
    AC167978
    Consensus CDS
    CCDS21109.1
    UniProtKB/Swiss-Prot
    Q60650, Q64467
    UniProtKB/TrEMBL
    A0A0R4J0X7
    Related
    ENSMUSP00000074317.5, ENSMUST00000074758.6
    Conserved Domains (3) summary
    COG0057
    Location:108 → 438
    GapA; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase [Carbohydrate transport and metabolism]
    pfam00044
    Location:108 → 254
    Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain
    pfam02800
    Location:259 → 416
    Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000073.7 Reference GRCm39 C57BL/6J

    Range
    30429204..30443106 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q64467.1 GenPept UniProtKB/Swiss-Prot:Q64467

Gene LinkOut

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