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Nek8 NIMA (never in mitosis gene a)-related expressed kinase 8 [ Mus musculus (house mouse) ]

Gene ID: 140859, updated on 21-Apr-2024

Summary

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Official Symbol
Nek8provided by MGI
Official Full Name
NIMA (never in mitosis gene a)-related expressed kinase 8provided by MGI
Primary source
MGI:MGI:1890646
See related
Ensembl:ENSMUSG00000017405 AllianceGenome:MGI:1890646
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
jck; b2b1449Clo; 4632401F23Rik
Summary
This gene encodes a NIMA-related kinase. Members of this serine/threonine protein kinase family are structurally-related to NIMA (never in mitosis, gene A) which controls mitotic signaling in Aspergillus nidulans. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in ovary adult (RPKM 10.2), adrenal adult (RPKM 9.9) and 28 other tissues See more
Orthologs
human all
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Genomic context

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Location:
11 B5; 11 46.74 cM
Exon count:
15
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 11 NC_000077.7 (78056932..78067501, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 11 NC_000077.6 (78166106..78176695, complement)

Chromosome 11 - NC_000077.7Genomic Context describing neighboring genes Neighboring gene family with sequence similarity 222, member B Neighboring gene U6 small nuclear RNA associated LSM6 pseudogene Neighboring gene STARR-positive B cell enhancer ABC_E10694 Neighboring gene STARR-seq mESC enhancer starr_30088 Neighboring gene TNF receptor associated factor 4 Neighboring gene STARR-positive B cell enhancer ABC_E8429 Neighboring gene STARR-positive B cell enhancer ABC_E9831 Neighboring gene STARR-positive B cell enhancer ABC_E7023 Neighboring gene microRNA 7653 Neighboring gene small nucleolar RNA, C/D box 42A Neighboring gene TLC domain containing 1 Neighboring gene ribosomal protein L23A

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Defective interplay between mTORC1 activity and endoplasmic reticulum stress-unfolded protein response in uremic vascular calcification. Panda DK, et al. Am J Physiol Renal Physiol, 2018 Jun 1. PMID 29357413
  2. CaMKII as a pathological mediator of ER stress, oxidative stress, and mitochondrial dysfunction in a murine model of nephronophthisis. Bracken C, et al. Am J Physiol Renal Physiol, 2016 Jun 1. PMID 27076647
  3. Development of polycystic kidney disease in juvenile cystic kidney mice: insights into pathogenesis, ciliary abnormalities, and common features with human disease. Smith LA, et al. J Am Soc Nephrol, 2006 Oct. PMID 16928806
  4. Ultrasound detection and characterization of polycystic kidney disease in a mouse model. Pollard R, et al. Comp Med, 2006 Jun. PMID 16774131
  5. Genetic localization of interacting modifiers affecting severity in a murine model of polycystic kidney disease. Kuida S, et al. Genome Res, 2000 Jan. PMID 10645949, Free PMC Article

See all (58) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Certain heterozygous variants in the kinase domain of the serine/threonine kinase NEK8 can cause an autosomal dominant form of polycystic kidney disease.
    Title: Certain heterozygous variants in the kinase domain of the serine/threonine kinase NEK8 can cause an autosomal dominant form of polycystic kidney disease.
  2. We constructed sub-congenic lines to more specifically localize the modifier and have determined it resides in a 3.2 Mb interval containing 28 genes. These include Invs and Anks6, which are both excellent candidates for the modifier as mutations in these genes result in PKD and both genes are known to genetically and physically interact with Nek8.
    Title: High-resolution genetic localization of a modifying locus affecting disease severity in the juvenile cystic kidneys (jck) mouse model of polycystic kidney disease.
  3. NEK8 plays a critical role in replication fork stability through its regulation of the DNA repair and replication fork protection protein RAD51.
    Title: NEK8 regulates DNA damage-induced RAD51 foci formation and replication fork protection.
  4. NEK8-depleted inner medullary collecting duct cells exhibited a defective response to fluid shear.
    Title: Loss of the ciliary kinase Nek8 causes left-right asymmetry defects.
  5. Nek8 acts downstream of Inv function
    Title: The ciliary protein Nek8/Nphp9 acts downstream of Inv/Nphp2 during pronephros morphogenesis and left-right establishment in zebrafish.
  6. Inv acts as a molecular anchor for Nphp3 and Nek8 in the proximal segment of primary cilia.
    Title: Inv acts as a molecular anchor for Nphp3 and Nek8 in the proximal segment of primary cilia.
  7. Defects in ciliary localization of Nek8 is associated with polycystic kidney diseases
    Title: Defects in ciliary localization of Nek8 is associated with cystogenesis.
  8. interacts with the signal transduction pathways of the polycystins and may lead to cystogenesis by altering the structure and function of cilia in the distal nephron
    Title: Nek8 regulates the expression and localization of polycystin-1 and polycystin-2.
  9. Nek8 and Pkd1 genes function in a common pathway to regulate cystogenesis. Moreover, compound Pkd1 and Nek8 heterozygous adult mice develop a more aggressive cystic disease than animals with a mutation in either gene alone.
    Title: Pkd1 and Nek8 mutations affect cell-cell adhesion and cilia in cysts formed in kidney organ cultures.
  10. cross-species analysis between zebrafish and mouse demonstrates taht NEK8 has a role in development of pronephric cysts
    Title: A defect in a novel Nek-family kinase causes cystic kidney disease in the mouse and in zebrafish.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Spontaneous (1)  1 citation
  • Targeted (1)  1 citation
  • Chemically induced (ENU) (2) 
  • Endonuclease-mediated (1) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein serine/threonine kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in animal organ morphogenesis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in animal organ morphogenesis ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within determination of left/right symmetry IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within heart development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within phosphorylation IEA
Inferred from Electronic Annotation
more info
  
involved_in protein phosphorylation IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of hippo signaling ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
located_in cell projection IEA
Inferred from Electronic Annotation
more info
  
located_in ciliary base IDA
Inferred from Direct Assay
more info
 PubMed 
located_in ciliary inversin compartment IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cilium IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cilium IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cilium ISO
Inferred from Sequence Orthology
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
serine/threonine-protein kinase Nek8
Names
NIMA-related expressed kinase 8
juvenile cystic kidney
never in mitosis A-related kinase 8
nimA-related protein kinase 8
NP_543125.1
XP_006532246.1
XP_006532247.1
XP_006532248.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_080849.3 → NP_543125.1  serine/threonine-protein kinase Nek8

    See identical proteins and their annotated locations for NP_543125.1

    Status: REVIEWED

    Source sequence(s)
    AF407579, BB646522, BC070457
    Consensus CDS
    CCDS25091.1
    UniProtKB/Swiss-Prot
    Q3U498, Q91ZR4, Q9D685
    Related
    ENSMUSP00000017549.7, ENSMUST00000017549.13
    Conserved Domains (4) summary
    smart00220
    Location:4 → 256
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    COG5184
    Location:318 → 663
    ATS1; Alpha-tubulin suppressor and related RCC1 domain-containing proteins [Cell cycle control, cell division, chromosome partitioning, Cytoskeleton]
    cd08220
    Location:3 → 258
    STKc_Nek8; Catalytic domain of the Protein Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase 8
    pfam00415
    Location:585 → 634
    RCC1; Regulator of chromosome condensation (RCC1) repeat

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000077.7 Reference GRCm39 C57BL/6J

    Range
    78056932..78067501 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006532184.5 → XP_006532247.1  serine/threonine-protein kinase Nek8 isoform X2

    Conserved Domains (2) summary
    COG5184
    Location:318 → 663
    ATS1; Alpha-tubulin suppressor and related RCC1 domain-containing proteins [Cell cycle control, cell division, chromosome partitioning, Cytoskeleton]
    cd08220
    Location:3 → 258
    STKc_Nek8; Catalytic domain of the Protein Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase 8

  2. XM_006532185.5 → XP_006532248.1  serine/threonine-protein kinase Nek8 isoform X3

    Conserved Domains (2) summary
    COG5184
    Location:274 → 619
    ATS1; Alpha-tubulin suppressor and related RCC1 domain-containing proteins [Cell cycle control, cell division, chromosome partitioning, Cytoskeleton]
    cl21453
    Location:3 → 214
    PKc_like; Protein Kinases, catalytic domain

  3. XM_006532183.5 → XP_006532246.1  serine/threonine-protein kinase Nek8 isoform X1

    Conserved Domains (2) summary
    COG5184
    Location:318 → 663
    ATS1; Alpha-tubulin suppressor and related RCC1 domain-containing proteins [Cell cycle control, cell division, chromosome partitioning, Cytoskeleton]
    cd08220
    Location:3 → 258
    STKc_Nek8; Catalytic domain of the Protein Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase 8
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q91ZR4.1 GenPept UniProtKB/Swiss-Prot:Q91ZR4

Gene LinkOut

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