Drosha drosha, ribonuclease type III [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Droshaprovided by MGI
Official Full Name: drosha, ribonuclease type IIIprovided by MGI
Primary source: MGI:MGI:1261425
See related: Ensembl:ENSMUSG00000022191 AllianceGenome:MGI:1261425
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Rn3; Etohi2; Rnasen; 1110013A17Rik
Summary: Enables endoribonuclease activity and primary miRNA binding activity. Involved in positive regulation of gene expression. Acts upstream of or within several processes, including ncRNA metabolic process; regulation of miRNA metabolic process; and regulation of regulatory T cell differentiation. Located in postsynaptic density. Is expressed in several structures, including central nervous system; early embryo; genitourinary system; hemolymphoid system gland; and tooth. Orthologous to human DROSHA (drosha ribonuclease III). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 18.2), CNS E14 (RPKM 14.7) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 15 A1; 15 5.99 cM

Exon count:: 36

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (12824893..12935377)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (12824804..12935291)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Global miRNA dosage control of embryonic germ layer specification.
Title: Global miRNA dosage control of embryonic germ layer specification.
Comparison of the miRNA profiles between wildtype and SUN1 null myotubes identified a cluster of miRNAs encoded by a non-translated retrotransposon-like one antisense (Rtl1as) transcript that are decreased in the WT myoblasts due to SUN1 inhibition of Drosha
Title: The mammalian LINC complex component SUN1 regulates muscle regeneration by modulating drosha activity.
Drosha plays a critical role in the survival of dopaminergic neurons.
Title: Loss of Drosha underlies dopaminergic neuron toxicity in models of Parkinson's disease.
Administration of bovine milk exosomes failed to rescue Drosha homozygous knockout mice, presumably due to low bioavailability or lack of essential microRNAs
Title: Milk exosomes are bioavailable and distinct microRNA cargos have unique tissue distribution patterns.
Study in mouse lines with either a targeted deletion of Drosha or an inducible expression of human DROSHA carrying a Wilms tumor (WT)-specific E1147K mutation that acts in a dominant negative manner underscores the importance of a viable self-renewing progenitor pool for kidney development, but there was no evidence of WT formation through impaired DROSHA function.
Title: Loss or oncogenic mutation of DROSHA impairs kidney development and function, but is not sufficient for Wilms tumor formation.
Mice lacking Drosha in the vascular endothelium developed a vascular phenotype resembling hereditary hemorrhagic telangiectasia that included dilated and disorganized vasculature, arteriovenous fistulae, and hemorrhages.
Title: Inactivating mutations in Drosha mediate vascular abnormalities similar to hereditary hemorrhagic telangiectasia.
Drosha knockout indicated a role for let-7 miRNAs in developmental hematopoiesis.
Title: Let-7 microRNA-dependent control of leukotriene signaling regulates the transition of hematopoietic niche in mice.
Identification of Microprocessor component DROSHA as a novel DNMT1-interactor.
Title: A novel requirement for DROSHA in maintenance of mammalian CG methylation.
Results show that Mammalian DROSHA genes have evolved a conserved hairpin structure spanning a specific exon-intron junction serving as a substrate for the microprocessor in human but not in murine cells.
Title: DROSHA targets its own transcript to modulate alternative splicing.
Knockdown of NFIB in Drosha-deficient hippocampal neural stem cells restores neurogenesis, suggesting that the Drosha/NFIB mechanism robustly prevents oligodendrocyte fate acquisition in vivo.
Title: Multipotency of Adult Hippocampal NSCs In Vivo Is Restricted by Drosha/NFIB.

Submit: New GeneRIF Correction See all GeneRIFs (29)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (6) 1 citation
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Etohi2 (e-PCR), detects polymorphism
- UniSTS:461893

D15Mit199 (e-PCR), detects polymorphism
- UniSTS:127565

Rnasen (e-PCR), detects polymorphism
- UniSTS:527519

NoName (e-PCR)
- UniSTS:237270

AI874853 (e-PCR)
- UniSTS:164574

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DEAD/H-box RNA helicase binding	ISO Inferred from Sequence Orthology more info
enables R-SMAD binding	ISO Inferred from Sequence Orthology more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables SMAD binding	ISO Inferred from Sequence Orthology more info
enables endonuclease activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables lipopolysaccharide binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nuclease activity	IEA Inferred from Electronic Annotation more info
enables primary miRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables primary miRNA binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables ribonuclease III activity	IBA Inferred from Biological aspect of Ancestor more info
enables ribonuclease III activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in RNA processing	IEA Inferred from Electronic Annotation more info
involved_in defense response to Gram-negative bacterium	ISO Inferred from Sequence Orthology more info
involved_in defense response to Gram-positive bacterium	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within miRNA metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pre-miRNA processing	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within pre-miRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in primary miRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in primary miRNA processing	ISO Inferred from Sequence Orthology more info
involved_in rRNA processing	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of miRNA metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of regulatory T cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within ribosome biogenesis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IEP Inferred from Expression Pattern more info	PubMed
part_of microprocessor complex	IBA Inferred from Biological aspect of Ancestor more info
part_of microprocessor complex	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
located_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic density	IEP Inferred from Expression Pattern more info	PubMed

General protein information

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Preferred Names: ribonuclease 3

Names: RNase III; ethanol induced 2; protein Drosha; ribonuclease III, nuclear

NP_001123621.1

EC 3.1.26.3

NP_081075.3

EC 3.1.26.3

XP_006520084.1

EC 3.1.26.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001130149.1 → NP_001123621.1 ribonuclease 3

See identical proteins and their annotated locations for NP_001123621.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.

Source sequence(s)

AK144147, BC088999, CA328055, U94841

Consensus CDS

CCDS49583.1

UniProtKB/Swiss-Prot

Q5HZJ0

UniProtKB/TrEMBL

Q6PAJ6

Related

ENSMUSP00000129279.2, ENSMUST00000169061.8

Conserved Domains (5) summary

smart00358
Location:1260 → 1331

DSRM; Double-stranded RNA binding motif

PRK00102
Location:1104 → 1332

rnc; ribonuclease III; Reviewed

cd00593
Location:1121 → 1253

RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...

pfam08648
Location:217 → 327

DUF1777; Protein of unknown function (DUF1777)

pfam12871
Location:209 → 289

PRP38_assoc; Pre-mRNA-splicing factor 38-associated hydrophilic C-term
NM_026799.3 → NP_081075.3 ribonuclease 3

See identical proteins and their annotated locations for NP_081075.3

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.

Source sequence(s)

BC088999, CA328055, U94841

Consensus CDS

CCDS49583.1

UniProtKB/Swiss-Prot

Q5HZJ0

UniProtKB/TrEMBL

Q6PAJ6

Related

ENSMUSP00000087762.6, ENSMUST00000090292.13

Conserved Domains (5) summary

smart00358
Location:1260 → 1331

DSRM; Double-stranded RNA binding motif

PRK00102
Location:1104 → 1332

rnc; ribonuclease III; Reviewed

cd00593
Location:1121 → 1253

RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...

pfam08648
Location:217 → 327

DUF1777; Protein of unknown function (DUF1777)

pfam12871
Location:209 → 289

PRP38_assoc; Pre-mRNA-splicing factor 38-associated hydrophilic C-term

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000081.7 Reference GRCm39 C57BL/6J

Range

12824893..12935377

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006520021.4 → XP_006520084.1 ribonuclease 3 isoform X1

UniProtKB/TrEMBL

Q6PAJ6

Conserved Domains (3) summary

PRK00102
Location:1124 → 1352

rnc; ribonuclease III; Reviewed

cd00593
Location:978 → 1098

RIBOc; Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in post-transcriptional ...

pfam12871
Location:231 → 300

PRP38_assoc; Pre-mRNA-splicing factor 38-associated hydrophilic C-term