ADORA3 adenosine A3 receptor [Homo sapiens (human)] - Gene

Summary

Official Symbol: ADORA3provided by HGNC
Official Full Name: adenosine A3 receptorprovided by HGNC
Primary source: HGNC:HGNC:268
See related: Ensembl:ENSG00000282608 MIM:600445; AllianceGenome:HGNC:268
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: A3AR
Summary: This gene encodes a protein that belongs to the family of adenosine receptors, which are G-protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. The receptor encoded by this gene mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it has been implicated in both neuroprotective and neurodegenerative effects, and it may also mediate both cell proliferation and cell death. Alternative splicing results in multiple transcript variants. This gene shares its 5' terminal exon with some transcripts from overlapping GeneID:57413, which encodes an immunoglobulin domain-containing protein. [provided by RefSeq, Nov 2014]
Expression: Broad expression in adrenal (RPKM 4.4), small intestine (RPKM 2.9) and 20 other tissues See more
Orthologs: all
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Genomic context

Location:: 1p13.2

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	1	NC_000001.11 (111499429..111503633, complement)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	1	NC_060925.1 (111514110..111518318, complement)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	1	NC_000001.10 (112042051..112046255, complement)

Chromosome 1 - NC_000001.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Increased expression of adenosine A3 receptor in tumor-infiltrating natural killer cells.
Title: Increased expression of adenosine A3 receptor in tumor-infiltrating natural killer cells.
Uncovering the Mechanisms of Adenosine Receptor-Mediated Pain Control: Focus on the A3 Receptor Subtype.
Title: Uncovering the Mechanisms of Adenosine Receptor-Mediated Pain Control: Focus on the A(3) Receptor Subtype.
Analysis of association of ADORA2A and ADORA3 polymorphisms genotypes/haplotypes with efficacy and toxicity of methotrexate in patients with Rheumatoid arthritis.
Title: Analysis of association of ADORA(2)A and ADORA(3) polymorphisms genotypes/haplotypes with efficacy and toxicity of methotrexate in patients with Rheumatoid arthritis.
Systematic deletion of adenosine receptors reveals novel roles in inflammation and pyroptosis in THP-1 macrophages.
Title: Systematic deletion of adenosine receptors reveals novel roles in inflammation and pyroptosis in THP-1 macrophages.
N(6)-methyladenosine (m(6)A) is an endogenous A3 adenosine receptor ligand.
Title: N(6)-methyladenosine (m(6)A) is an endogenous A3 adenosine receptor ligand.
Activation of Adenosine A3 Receptor Inhibits Microglia Reactivity Elicited by Elevated Pressure.
Title: Activation of Adenosine A(3) Receptor Inhibits Microglia Reactivity Elicited by Elevated Pressure.
Adenosine A2A and A3 Receptors Are Able to Interact with Each Other. A Further Piece in the Puzzle of Adenosine Receptor-Mediated Signaling.
Title: Adenosine A(2A) and A(3) Receptors Are Able to Interact with Each Other. A Further Piece in the Puzzle of Adenosine Receptor-Mediated Signaling.
A3 adenosine receptor activation mechanisms: molecular dynamics analysis of inactive, active, and fully active states.
Title: A(3) adenosine receptor activation mechanisms: molecular dynamics analysis of inactive, active, and fully active states.
Results showed that such modification diminished the A2A activity and instead conferred hA3AR agonistic activity. Among the new mIPP derivatives (3-6), compound 4 showed potential as a hA3AR partial agonist, with an Emax of 30% and EC50 of 2.89 +/- 0.55 muM.
Title: Design, Synthesis and Evaluation of New Indolylpyrimidylpiperazines for Gastrointestinal Cancer Therapy.
TLR-Induced IL-12 and CCL2 Production by Myeloid Cells Is Dependent on Adenosine A3 Receptor-Mediated Signaling.
Title: TLR-Induced IL-12 and CCL2 Production by Myeloid Cells Is Dependent on Adenosine A(3) Receptor-Mediated Signaling.

Submit: New GeneRIF Correction See all GeneRIFs (67)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Envelope transmembrane glycoprotein gp41	env	A synthetic peptide corresponding to the immunosuppressive domain (amino acids 574-592) of HIV-1 gp41 downregulates the expression of adenosine A3 receptor (ADORA3) in peptide-treated PBMCs	PubMed
Tat	tat	HIV-1 Tat upregulates adenosine A3 receptor in HEK 293T cells	PubMed

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

GDB:632846 (e-PCR)
- UniSTS:158496

ADORA3 (e-PCR)
- UniSTS:266362

SHGC-12531 (e-PCR)
- UniSTS:40363

RH69986 (e-PCR)
- UniSTS:5106

ADORA3_1557 (e-PCR)
- UniSTS:276978

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables G protein-coupled adenosine receptor activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in G protein-coupled adenosine receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in activation of adenylate cyclase activity	TAS Traceable Author Statement more info	PubMed
involved_in inflammatory response	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of NF-kappaB transcription factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in presynaptic modulation of chemical synaptic transmission	IEA Inferred from Electronic Annotation more info
involved_in regulation of heart contraction	TAS Traceable Author Statement more info	PubMed
involved_in regulation of norepinephrine secretion	IEA Inferred from Electronic Annotation more info
involved_in response to wounding	TAS Traceable Author Statement more info	PubMed
involved_in signal transduction	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in Schaffer collateral - CA1 synapse	IEA Inferred from Electronic Annotation more info
is_active_in dendrite	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	TAS Traceable Author Statement more info
located_in presynaptic membrane	IEA Inferred from Electronic Annotation more info
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: adenosine receptor A3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_032119.1 RefSeqGene

Range

5001..85628

Download

GenBank, FASTA, Sequence Viewer (Graphics), LRG_424

mRNA and Protein(s)

NM_000677.4 → NP_000668.1 adenosine receptor A3 isoform A

See identical proteins and their annotated locations for NP_000668.1

Status: REVIEWED

Description

Transcript Variant: This variant (A) encodes the longest isoform (A).

Source sequence(s)

AL390195

Consensus CDS

CCDS839.1

UniProtKB/Swiss-Prot

A2A3P4, P0DMS8, P33765, Q6UWU0, Q9BYZ1

UniProtKB/TrEMBL

B2R7S2, H6VQ59

Related

ENSP00000241356.4, ENST00000241356.5

Conserved Domains (2) summary

cd14964
Location:15 → 39

7tm_GPCRs; TM helix 1 [structural motif]

cl28897
Location:13 → 293

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily
NM_001302678.2 → NP_001289607.1 adenosine receptor A3 isoform B

Status: REVIEWED

Description

Transcript Variant: This variant (B) uses an alternate splice site in the 3' terminal exon, resulting in an alternate 3' coding region and 3' UTR, compared to variant A. The encoded isoform (B) has a distinct C-terminus and is shorter than isoform A.

Source sequence(s)

AL390195, BF432324, BM675096, CD013875, JN855714

Consensus CDS

CCDS81358.1

UniProtKB/TrEMBL

A0A0J9YWR0

Related

ENSP00000488073.1, ENST00000632535.1

Conserved Domains (2) summary

cd14964
Location:14 → 40

7tm_GPCRs; TM helix 1 [structural motif]

cl28897
Location:13 → 116

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily
NM_001302679.2 → NP_001289608.1 adenosine receptor A3 isoform C

Status: REVIEWED

Description

Transcript Variant: This variant (C) uses an alternate splice site in the 5' terminal exon, and it thus differs in its 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant A. The encoded isoform (C) is shorter at the N-terminus, compared to isoform A.

Source sequence(s)

AL390195, BF432324, BM675096, BX283401, JN855714

UniProtKB/TrEMBL

H6VQ59

Conserved Domains (2) summary

cd14964
Location:29 → 59

7tm_GPCRs; TM helix 5 [structural motif]

cl28897
Location:1 → 148

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily