Ephx1 epoxide hydrolase 1, microsomal [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ephx1provided by MGI
Official Full Name: epoxide hydrolase 1, microsomalprovided by MGI
Primary source: MGI:MGI:95405
See related: Ensembl:ENSMUSG00000038776 AllianceGenome:MGI:95405
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: EH; mEH; Eph1; Eph-1; Epxh1
Summary: Predicted to enable cis-stilbene-oxide hydrolase activity; enzyme binding activity; and epoxide hydrolase activity. Acts upstream of or within cellular aromatic compound metabolic process and response to organic cyclic compound. Predicted to be located in intracellular membrane-bounded organelle and plasma membrane. Is expressed in several structures, including brain ventricle and choroid plexus; cranial ganglion; integumental system; nose; and salivary gland. Human ortholog(s) of this gene implicated in several diseases, including Leber hereditary optic neuropathy; anemia (multiple); hematologic cancer (multiple); respiratory system disease (multiple); and toxic encephalopathy. Orthologous to human EPHX1 (epoxide hydrolase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in liver adult (RPKM 113.7), bladder adult (RPKM 62.6) and 19 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 H4; 1 84.48 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (180817121..180845134, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (180989556..181017569, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Soluble Epoxide Hydrolase in Aged Female Mice and Human Explanted Hearts Following Ischemic Injury.
Title: Soluble Epoxide Hydrolase in Aged Female Mice and Human Explanted Hearts Following Ischemic Injury.
Microsomal epoxide hydrolase forms complex with CYP2J5 in the endoplasmic reticulum.
Title: Evidence for a complex formation between CYP2J5 and mEH in living cells by FRET analysis of membrane protein interaction in the endoplasmic reticulum (FAMPIR).
High epoxide hydrolase expression is associated with colonic inflammation.
Title: Lipidomic profiling reveals soluble epoxide hydrolase as a therapeutic target of obesity-induced colonic inflammation.
MEH appears to play a leading role in the hydrolysis of 8,9-epoxyeicosatrienoic acid and also contributes to the hydrolysis of other fatty acid epoxides.
Title: Beyond detoxification: a role for mouse mEH in the hepatic metabolism of endogenous lipids.
These findings show the critical role of soluble epoxide hydrolase S-nitrosation in cardiac ischemia-reperfusion injury.
Title: Soluble epoxide hydrolase activation by S-nitrosation contributes to cardiac ischemia-reperfusion injury.
Data, including data from mutant strain of mice, suggest critical role of Ephx1 in regulation of vasodynamics (specifically regulation of cerebral blood flow) and suggest that deregulation of endogenous signaling pathways (such as eicosanoid metabolism) is undesirable gain of function associated with E404D variant of Ephx1.
Title: Genetic enhancement of microsomal epoxide hydrolase improves metabolic detoxification but impairs cerebral blood flow regulation.
Six proteins were regulated at both basal and inducible levels exhibiting the largest dynamic range of Nrf2 regulation: cytochrome CYP2A5, GSTM3, GSTM1, ENTPD5,UDPGDH, and EPHX1.
Title: Identification and quantification of the basal and inducible Nrf2-dependent proteomes in mouse liver: biochemical, pharmacological and toxicological implications.
Expression of HBSP significantly increased the formation of BPDE-DNA adduct in benzo[alpha]pyrene-treated Huh-7 hepatoma cells, and this enhancement was blocked by knockdown of microsomal epoxide hydrolase
Title: Hepatitis B spliced protein (HBSP) promotes the carcinogenic effects of benzo [alpha] pyrene by interacting with microsomal epoxide hydrolase and enhancing its hydrolysis activity.
In livers of HRN mice Cyp1a1, cytochrome b5 and mEH can effectively activate BaP to DNA binding species, even in the presence of very low amounts of P450 oxidoreductase.
Title: Cytochrome b5 and epoxide hydrolase contribute to benzo[a]pyrene-DNA adduct formation catalyzed by cytochrome P450 1A1 under low NADPH:P450 oxidoreductase conditions.
The results suggest that epoxide intermediates mediate methamphetamine drug dependence and that astrocytic reactions of microsomal epoxide hydrolase protein are important in the endogenous modulation in response to methamphetamine drug dependence.
Title: Role of microsomal epoxide hydrolase in methamphetamine-induced drug dependence in mice.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (4)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC99591P2 (e-PCR)
- UniSTS:273684

EPHX1 (e-PCR)
- UniSTS:278342

Ephx1 (e-PCR), detects polymorphism
- UniSTS:186694

AI195553 (e-PCR)
- UniSTS:185460

UniSTS:142997 (e-PCR)
- UniSTS:142997

D1Mit151 (e-PCR), detects polymorphism
- UniSTS:128489

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables cis-stilbene-oxide hydrolase activity	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables epoxide hydrolase activity	IBA Inferred from Biological aspect of Ancestor more info
enables epoxide hydrolase activity	ISO Inferred from Sequence Orthology more info
enables ether hydrolase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables oxysterol binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in arachidonic acid metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in arachidonic acid metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within aromatic compound catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular aromatic compound metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in diol biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in epoxide metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in epoxide metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to organic cyclic compound	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to toxic substance	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: epoxide hydrolase 1

Names: epoxide hydratase; microsomal epoxide hydrolase

NP_001299847.1

EC 3.3.2.9

NP_034275.1

EC 3.3.2.9

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001312918.1 → NP_001299847.1 epoxide hydrolase 1 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

Source sequence(s)

AC117673, AK018249, AW047588, BY312366

Consensus CDS

CCDS83653.1

UniProtKB/TrEMBL

E9PWK1, Q6PEV0

Related

ENSMUSP00000106697.2, ENSMUST00000111068.8

Conserved Domains (3) summary

COG0596
Location:121 → 434

MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]

pfam06441
Location:50 → 152

EHN; Epoxide hydrolase N terminus

cl21494
Location:142 → 231

Abhydrolase; alpha/beta hydrolases
NM_010145.3 → NP_034275.1 epoxide hydrolase 1 isoform 1

See identical proteins and their annotated locations for NP_034275.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC117673, AK018249, AW047588, BY312366

Consensus CDS

CCDS15579.1

UniProtKB/Swiss-Prot

P97869, Q9D379

UniProtKB/TrEMBL

Q8K2W5

Related

ENSMUSP00000047551.6, ENSMUST00000036928.12

Conserved Domains (3) summary

COG0596
Location:121 → 448

MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]

pfam06441
Location:50 → 152

EHN; Epoxide hydrolase N terminus

cl21494
Location:124 → 255

Abhydrolase; alpha/beta hydrolases