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Ebf2 early B cell factor 2 [ Mus musculus (house mouse) ]

Gene ID: 13592, updated on 21-Apr-2024

Summary

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Official Symbol
Ebf2provided by MGI
Official Full Name
early B cell factor 2provided by MGI
Primary source
MGI:MGI:894332
See related
Ensembl:ENSMUSG00000022053 AllianceGenome:MGI:894332
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
OE-3; EBF-2; Mmot1; O/E-3; D14Ggc1e
Summary
Enables DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and chromatin binding activity. Involved in positive regulation of cold-induced thermogenesis and positive regulation of transcription by RNA polymerase II. Acts upstream of or within several processes, including adipose tissue development; brown fat cell differentiation; and positive regulation of chromatin binding activity. Predicted to be located in nucleus. Is expressed in several structures, including branchial arch; central nervous system; genitourinary system; limb mesenchyme; and sensory organ. Orthologous to human EBF2 (EBF transcription factor 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in limb E14.5 (RPKM 7.4), CNS E11.5 (RPKM 7.0) and 10 other tissues See more
Orthologs
human all
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Genomic context

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Location:
14 D1; 14 34.63 cM
Exon count:
17
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 14 NC_000080.7 (67470741..67668401)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 14 NC_000080.6 (67233292..67430952)

Chromosome 14 - NC_000080.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 30806 Neighboring gene STARR-seq mESC enhancer starr_37010 Neighboring gene predicted gene 20675 Neighboring gene predicted gene, 52149 Neighboring gene RIKEN cDNA 4930438E09 gene Neighboring gene predicted gene 6878 Neighboring gene STARR-seq mESC enhancer starr_37011 Neighboring gene predicted gene 10860 Neighboring gene STARR-seq mESC enhancer starr_37012 Neighboring gene predicted gene, 38409 Neighboring gene predicted gene, 24981

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Motor dysfunction and cerebellar Purkinje cell firing impairment in Ebf2 null mice. Hoxha E, et al. Mol Cell Neurosci, 2013 Jan. PMID 23000673
  2. Ebf2 marks early cortical neurogenesis and regulates the generation of cajal-retzius neurons in the developing cerebral cortex. Chuang SM, et al. Dev Neurosci, 2011. PMID 22042145
  3. Aspects of the narcolepsy-cataplexy syndrome in O/E3-null mutant mice. De La Herrán-Arita AK, et al. Neuroscience, 2011 Jun 2. PMID 21435382
  4. Both Schwann cell and axonal defects cause motor peripheral neuropathy in Ebf2-/- mice. Giacomini C, et al. Neurobiol Dis, 2011 Apr. PMID 21220016
  5. A t(2;8) balanced translocation with breakpoints near the human HOXD complex causes mesomelic dysplasia and vertebral defects. Spitz F, et al. Genomics, 2002 Apr. PMID 11944980

See all (101) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Age-Progressive and Gender-Dependent Bone Phenotype in Mice Lacking Both Ebf1 and Ebf2 in Prrx1-Expressing Mesenchymal Cells.
    Title: Age-Progressive and Gender-Dependent Bone Phenotype in Mice Lacking Both Ebf1 and Ebf2 in Prrx1-Expressing Mesenchymal Cells.
  2. ZFP423 controls EBF2 coactivator recruitment and PPARgamma occupancy to determine the thermogenic plasticity of adipocytes.
    Title: ZFP423 controls EBF2 coactivator recruitment and PPARγ occupancy to determine the thermogenic plasticity of adipocytes.
  3. Early B Cell Factor Activity Controls Developmental and Adaptive Thermogenic Gene Programming in Adipocytes.
    Title: Early B Cell Factor Activity Controls Developmental and Adaptive Thermogenic Gene Programming in Adipocytes.
  4. Using genetic fate mapping, it was determined that Ebf2 mRNA is initially found in all Purkinje cell progenitors during cerebellar development.
    Title: Dynamic Expression and New Functions of Early B Cell Factor 2 in Cerebellar Development.
  5. Down-regulation of EBF2 (and EBF1) genes leads to severe impairment of myelin formation in Schwann cells.
    Title: The Transcription Factors EBF1 and EBF2 Are Positive Regulators of Myelination in Schwann Cells.
  6. we show that SIX1 binds to adipogenic and brown marker genes and interacts with C/EBPa, C/EBPb and EBF2, suggesting their functional cooperation during adipogenesis.
    Title: Comparative Transcriptomic and Epigenomic Analyses Reveal New Regulators of Murine Brown Adipogenesis.
  7. Data indicate that Contactin-1 expression is dependent upon EBF factors; Cntn1 gene belongs to the expanding regulatory cascade driven by these transcriptional regulators so that changes in its activation may contribute to the Ebf2 null mutant phenotype
    Title: The gene encoding the mouse contactin-1 axonal glycoprotein is regulated by the collier/Olf1/EBF family early B-Cell factor 2 transcription factor.
  8. Study identified Ebf2 as a novel and important regulator selectively required for midbrain periaqueductal gray matter dopamine neuron development
    Title: Ebf2 is required for development of dopamine neurons in the midbrain periaqueductal gray matter of mouse.
  9. Results indicate that early B cell factor 2 (Ebf2) specifically marks and regulates the molecular profile of brown preadipose cells.
    Title: Ebf2 is a selective marker of brown and beige adipogenic precursor cells.
  10. Blnc1 forms a ribonucleoprotein complex with transcription factor EBF2 to stimulate the thermogenic gene program. Further, Blnc1 itself is a target of EBF2, thereby forming a feedforward regulatory loop to drive adipogenesis toward thermogenic phenotype.
    Title: A long noncoding RNA transcriptional regulatory circuit drives thermogenic adipocyte differentiation.
Submit: New GeneRIF Correction See all GeneRIFs (21)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (9)  1 citation
  • Endonuclease-mediated (1) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA-binding transcription activator activity, RNA polymerase II-specific IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA-binding transcription factor activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription factor activity, RNA polymerase II-specific IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables chromatin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within adipose tissue development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within brown fat cell differentiation IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within cell fate determination IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within multicellular organism development IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within positive regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within positive regulation of chromatin binding IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of cold-induced thermogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
transcription factor COE2
Names
MET-mesencephalon-olfactory TF1
MET-mesencephalon-olfactory transcription factor 1
Olf-1/EBF-like 3
metencephalon-mesencephalon-olfactory transcription factor 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001276387.1 → NP_001263316.1  transcription factor COE2

    See identical proteins and their annotated locations for NP_001263316.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.
    Source sequence(s)
    AK053013, AK139262, BB669324, CT010493
    Consensus CDS
    CCDS27228.1
    UniProtKB/Swiss-Prot
    O08792, Q543D5
    Related
    ENSMUSP00000135500.2, ENSMUST00000176161.8
    Conserved Domains (3) summary
    cd01175
    Location:253 → 337
    IPT_COE; IPT domain of the COE family (Col/Olf-1/EBF) of non-basic, helix-loop-helix (HLH)-containing transcription factors. COE family proteins are all transcription factors and play an important role in variety of developmental processes. Mouse EBF is involved ...
    pfam16422
    Location:20 → 246
    COE1_DBD; Transcription factor COE1 DNA-binding domain
    pfam16423
    Location:338 → 381
    COE1_HLH; Transcription factor COE1 helix-loop-helix domain

  2. NM_001360202.1 → NP_001347131.1  transcription factor COE2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) differs in 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein.
    Source sequence(s)
    BC050922, BM932499, CT010493
    Consensus CDS
    CCDS27228.1
    UniProtKB/Swiss-Prot
    O08792, Q543D5
    Related
    ENSMUSP00000135782.2, ENSMUST00000176029.2
    Conserved Domains (3) summary
    cd01175
    Location:253 → 337
    IPT_COE; IPT domain of the COE family (Col/Olf-1/EBF) of non-basic, helix-loop-helix (HLH)-containing transcription factors. COE family proteins are all transcription factors and play an important role in variety of developmental processes. Mouse EBF is involved ...
    pfam16422
    Location:20 → 246
    COE1_DBD; Transcription factor COE1 DNA-binding domain
    pfam16423
    Location:338 → 381
    COE1_HLH; Transcription factor COE1 helix-loop-helix domain

  3. NM_010095.6 → NP_034225.1  transcription factor COE2

    See identical proteins and their annotated locations for NP_034225.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) uses an alternate splice site in 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein.
    Source sequence(s)
    AK053013, AK139262, BB669324, CT010493
    Consensus CDS
    CCDS27228.1
    UniProtKB/Swiss-Prot
    O08792, Q543D5
    Related
    ENSMUSP00000022637.8, ENSMUST00000022637.14
    Conserved Domains (3) summary
    cd01175
    Location:253 → 337
    IPT_COE; IPT domain of the COE family (Col/Olf-1/EBF) of non-basic, helix-loop-helix (HLH)-containing transcription factors. COE family proteins are all transcription factors and play an important role in variety of developmental processes. Mouse EBF is involved ...
    pfam16422
    Location:20 → 246
    COE1_DBD; Transcription factor COE1 DNA-binding domain
    pfam16423
    Location:338 → 381
    COE1_HLH; Transcription factor COE1 helix-loop-helix domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000080.7 Reference GRCm39 C57BL/6J

    Range
    67470741..67668401
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O08792.4 GenPept UniProtKB/Swiss-Prot:O08792

Gene LinkOut

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