Ddx3x DEAD box helicase 3, X-linked [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ddx3xprovided by MGI
Official Full Name: DEAD box helicase 3, X-linkedprovided by MGI
Primary source: MGI:MGI:103064
See related: Ensembl:ENSMUSG00000000787 AllianceGenome:MGI:103064
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ddx3; Fin14; D1Pas1-rs2
Summary: Enables primary miRNA binding activity. Involved in positive regulation of NLRP3 inflammasome complex assembly and stress granule assembly. Acts upstream of or within with a negative effect on primary miRNA processing. Located in cytoplasmic stress granule. Part of NLRP3 inflammasome complex. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in syndromic X-linked intellectual disability. Orthologous to human DDX3X (DEAD-box helicase 3 X-linked). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in CNS E11.5 (RPKM 64.5), placenta adult (RPKM 53.4) and 23 other tissues See more
Orthologs: human all
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Genomic context

Location:: X A1.1; X 8.17 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	X	NC_000086.8 (13147261..13160222)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	X	NC_000086.7 (13280496..13293988)

Chromosome X - NC_000086.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A ubiquitous GC content signature underlies multimodal mRNA regulation by DDX3X.
Title: A ubiquitous GC content signature underlies multimodal mRNA regulation by DDX3X.
Cell-type-specific need of Ddx3 and PACT for interferon induction by RNA viruses.
Title: Cell-type-specific need of Ddx3 and PACT for interferon induction by RNA viruses.
DDX3X Deficiency Attenuates Pyroptosis Induced by Oxygen-glucose Deprivation/Reoxygenation in N2a Cells.
Title: DDX3X Deficiency Attenuates Pyroptosis Induced by Oxygen-glucose Deprivation/Reoxygenation in N2a Cells.
miR-181a-5p targets DDX3X to inhibit the progression of osteoarthritis via NF-KappaB signaling pathway.
Title: miR-181a-5p targets DDX3X to inhibit the progression of osteoarthritis via NF-ΚB signaling pathway.
Hepatocyte DDX3X protects against drug-induced acute liver injury via controlling stress granule formation and oxidative stress.
Title: Hepatocyte DDX3X protects against drug-induced acute liver injury via controlling stress granule formation and oxidative stress.
Overexpressed DDX3x promotes abdominal aortic aneurysm formation and activates AKT in ApoE knockout mice.
Title: Overexpressed DDX3x promotes abdominal aortic aneurysm formation and activates AKT in ApoE knockout mice.
The X-Linked Helicase DDX3X Is Required for Lymphoid Differentiation and MYC-Driven Lymphomagenesis.
Title: The X-Linked Helicase DDX3X Is Required for Lymphoid Differentiation and MYC-Driven Lymphomagenesis.
The DEAD-box helicase DDX3x ameliorates non-alcoholic fatty liver disease via mTORC1 signalling pathway.
Title: The DEAD-box helicase DDX3x ameliorates non-alcoholic fatty liver disease via mTORC1 signalling pathway.
Aberrant cortical development is driven by impaired cell cycle and translational control in a DDX3X syndrome model.
Title: Aberrant cortical development is driven by impaired cell cycle and translational control in a DDX3X syndrome model.
AKT controls NLRP3 inflammasome activation by inducing DDX3X phosphorylation.
Title: AKT controls NLRP3 inflammasome activation by inducing DDX3X phosphorylation.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (5) 1 citation
Endonuclease-mediated (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

DDX3 (e-PCR)
- UniSTS:278276

Fin14 (e-PCR), detects polymorphism
- UniSTS:143110

Ddx3 (e-PCR), detects polymorphism
- UniSTS:255281

U42386 (e-PCR)
- UniSTS:125746

Ddx3 (e-PCR), detects polymorphism
- UniSTS:256392

DBX (e-PCR)
- UniSTS:266902

L25126 (e-PCR)
- UniSTS:124886

Ddx3x (e-PCR), detects polymorphism
- UniSTS:506840

RH127853 (e-PCR)
- UniSTS:211163

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	ISO Inferred from Sequence Orthology more info
enables CTPase activity	ISO Inferred from Sequence Orthology more info
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables DNA helicase activity	ISO Inferred from Sequence Orthology more info
enables GTPase activity	ISO Inferred from Sequence Orthology more info
enables RNA binding	ISO Inferred from Sequence Orthology more info
enables RNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA helicase activity	ISO Inferred from Sequence Orthology more info
enables RNA stem-loop binding	ISO Inferred from Sequence Orthology more info
enables RNA strand annealing activity	ISO Inferred from Sequence Orthology more info
enables eukaryotic initiation factor 4E binding	ISO Inferred from Sequence Orthology more info
enables gamma-tubulin binding	ISO Inferred from Sequence Orthology more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables mRNA 5'-UTR binding	ISO Inferred from Sequence Orthology more info
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	ISO Inferred from Sequence Orthology more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables poly(A) binding	ISO Inferred from Sequence Orthology more info
enables primary miRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine/threonine kinase activator activity	ISO Inferred from Sequence Orthology more info
enables ribonucleoside triphosphate phosphatase activity	ISO Inferred from Sequence Orthology more info
enables ribosomal small subunit binding	ISO Inferred from Sequence Orthology more info
enables signaling adaptor activity	ISO Inferred from Sequence Orthology more info
enables transcription factor binding	ISO Inferred from Sequence Orthology more info
enables translation initiation factor binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in RNA secondary structure unwinding	ISO Inferred from Sequence Orthology more info
involved_in Wnt signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to arsenic-containing substance	ISO Inferred from Sequence Orthology more info
involved_in cellular response to osmotic stress	ISO Inferred from Sequence Orthology more info
involved_in cellular response to virus	ISO Inferred from Sequence Orthology more info
involved_in chromosome segregation	ISO Inferred from Sequence Orthology more info
involved_in cytosolic ribosome assembly	ISO Inferred from Sequence Orthology more info
involved_in extrinsic apoptotic signaling pathway via death domain receptors	ISO Inferred from Sequence Orthology more info
involved_in gamete generation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within immune system process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within inflammatory response	IEA Inferred from Electronic Annotation more info
involved_in innate immune response	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	ISO Inferred from Sequence Orthology more info
involved_in intrinsic apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in lipid homeostasis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell growth	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cysteine-type endopeptidase activity involved in apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of extrinsic apoptotic signaling pathway via death domain receptors	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of gene expression	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of intrinsic apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of non-canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein-containing complex assembly	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of translation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of G1/S transition of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of NLRP3 inflammasome complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical Wnt signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell growth	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cysteine-type endopeptidase activity involved in apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interferon-alpha production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interferon-beta production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of non-canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein K63-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein acetylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein autophosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of toll-like receptor 7 signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of toll-like receptor 8 signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of translation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of translation in response to endoplasmic reticulum stress	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of translational initiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of type I interferon production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of viral genome replication	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within_negative_effect primary miRNA processing	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within_negative_effect primary miRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to cytoplasmic stress granule	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in response to virus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within ribosome biogenesis	IEA Inferred from Electronic Annotation more info
involved_in stress granule assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in stress granule assembly	ISO Inferred from Sequence Orthology more info
involved_in translational initiation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of NLRP3 inflammasome complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in P granule	IBA Inferred from Biological aspect of Ancestor more info
part_of canonical inflammasome complex	IEA Inferred from Electronic Annotation more info
located_in cell leading edge	ISO Inferred from Sequence Orthology more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic stress granule	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic stress granule	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
part_of cytosolic small ribosomal subunit	ISO Inferred from Sequence Orthology more info
part_of eukaryotic translation initiation factor 3 complex	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: ATP-dependent RNA helicase DDX3X

Names: D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 3; D1Pas1-related sequence 2; DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 3; DEAD box RNA helicase DEAD3; DEAD box protein 3, X-chromosomal; DEAD-box protein 3 (DEAD-box RNA helicase DEAD3) (mDEAD3) (Embryonic RNA helicase) (D1PAS1 related sequence 2); DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked; embryonic RNA helicase; fibroblast growth factor inducible 14; mDEAD3

NP_034158.1

EC 3.6.4.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010028.3 → NP_034158.1 ATP-dependent RNA helicase DDX3X

See identical proteins and their annotated locations for NP_034158.1

Status: VALIDATED

Source sequence(s)

AK163250, AL833805, BB854842

Consensus CDS

CCDS30027.1

UniProtKB/Swiss-Prot

O09060, O09143, Q62167

UniProtKB/TrEMBL

B9EKE9, Q3TQX5

Related

ENSMUSP00000000804.7, ENSMUST00000000804.7

Conserved Domains (4) summary

cd00268
Location:182 → 404

DEADc; DEAD-box helicases. A diverse family of proteins involved in ATP-dependent RNA unwinding, needed in a variety of cellular processes including splicing, ribosome biogenesis and RNA degradation. The name derives from the sequence of the Walker B motif ...

PTZ00110
Location:57 → 580

PTZ00110; helicase; Provisional

pfam00271
Location:426 → 536

Helicase_C; Helicase conserved C-terminal domain

pfam06273
Location:80 → 172

eIF-4B; Plant specific eukaryotic initiation factor 4B

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000086.8 Reference GRCm39 C57BL/6J

Range

13147261..13160222

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_008015.1: Suppressed sequence

Description

NM_008015.1: This RefSeq record was removed by NCBI staff. Contact info@ncbi.nlm.nih.gov for further information.