Cttn cortactin [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cttnprovided by MGI
Official Full Name: cortactinprovided by MGI
Primary source: MGI:MGI:99695
See related: Ensembl:ENSMUSG00000031078 AllianceGenome:MGI:99695
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ems1; 1110020L01Rik
Summary: Predicted to enable several functions, including Arp2/3 complex binding activity; actin filament binding activity; and profilin binding activity. Involved in intracellular protein transport and regulation of autophagy of mitochondrion. Acts upstream of or within dendritic spine maintenance; negative regulation of extrinsic apoptotic signaling pathway; and substrate-dependent cell migration, cell extension. Located in several cellular components, including cytoskeleton; lamellipodium; and ruffle. Part of voltage-gated potassium channel complex. Is expressed in several structures, including 1-cell stage conceptus; central nervous system; genitourinary system; gut; and sensory organ. Orthologous to human CTTN (cortactin). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in bladder adult (RPKM 56.4), colon adult (RPKM 53.9) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 F5; 7 88.84 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (143989468..144024743, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (144435724..144471560, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Requirement of Site-Specific Tyrosine Phosphorylation of Cortactin in Retinal Neovascularization and Vascular Leakage.
Title: Requirement of Site-Specific Tyrosine Phosphorylation of Cortactin in Retinal Neovascularization and Vascular Leakage.
ERbeta promoted invadopodia formation-mediated non-small cell lung cancer metastasis via the ICAM1/p-Src/p-Cortactin signaling pathway.
Title: ERβ promoted invadopodia formation-mediated non-small cell lung cancer metastasis via the ICAM1/p-Src/p-Cortactin signaling pathway.
Cortactin Contributes to Activity-Dependent Modulation of Spine Actin Dynamics and Spatial Memory Formation.
Title: Cortactin Contributes to Activity-Dependent Modulation of Spine Actin Dynamics and Spatial Memory Formation.
Abl2:Cortactin Interactions Regulate Dendritic Spine Stability via Control of a Stable Filamentous Actin Pool.
Title: Abl2:Cortactin Interactions Regulate Dendritic Spine Stability via Control of a Stable Filamentous Actin Pool.
Actin-Binding Protein Cortactin Promotes Pathogenesis of Experimental Autoimmune Encephalomyelitis by Supporting Leukocyte Infiltration into the Central Nervous System.
Title: Actin-Binding Protein Cortactin Promotes Pathogenesis of Experimental Autoimmune Encephalomyelitis by Supporting Leukocyte Infiltration into the Central Nervous System.
Autism candidate gene DIP2A regulates spine morphogenesis via acetylation of cortactin.
Title: Autism candidate gene DIP2A regulates spine morphogenesis via acetylation of cortactin.
this paper shows that cortactin deficiency causes increased RhoA/ROCK1-dependent actomyosin contractility, intestinal epithelial barrier dysfunction, and disproportionately severe dextran sulfate sodium-induced colitis
Title: Cortactin deficiency causes increased RhoA/ROCK1-dependent actomyosin contractility, intestinal epithelial barrier dysfunction, and disproportionately severe DSS-induced colitis.
Secretion of the barrier-stabilising hormone adrenomedullin, which activates Rap1 and counteracts actomyosin contractility, was reduced in plasma from cortactin-deficient mice. Cortactin plays a role in controlling actomyosin contractility with consequences for endothelial barrier integrity.
Title: Loss of cortactin causes endothelial barrier dysfunction via disturbed adrenomedullin secretion and actomyosin contractility.
These findings suggest that the patterning of podosomes into a sealing zone involves the dynamic interaction between cofilin, CTTN, and the microtubule + ends.
Title: The Actin-Binding Protein Cofilin and Its Interaction With Cortactin Are Required for Podosome Patterning in Osteoclasts and Bone Resorption In Vivo and In Vitro.
AMPK phosphorylation of cortactin followed by SIRT1 deacetylation modulates the interaction of cortactin and cortical-actin in response to shear stress. Functionally, this AMPK/SIRT1 coregulated cortactin-F-actin dynamics is required for endothelial nitric oxide synthase subcellular translocation/activation and is atheroprotective.
Title: AMP-Activated Protein Kinase and Sirtuin 1 Coregulation of Cortactin Contributes to Endothelial Function.

Submit: New GeneRIF Correction See all GeneRIFs (51)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (6) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH126596 (e-PCR)
- UniSTS:209712

RH124771 (e-PCR)
- UniSTS:161593

RH136052 (e-PCR)
- UniSTS:210047

NoName (e-PCR)
- UniSTS:493492

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables Arp2/3 complex binding	ISO Inferred from Sequence Orthology more info
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables profilin binding	ISO Inferred from Sequence Orthology more info
enables proline-rich region binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in cell migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell motility	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within dendritic spine maintenance	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within endocytosis	IEA Inferred from Electronic Annotation more info
acts_upstream_of extrinsic apoptotic signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in focal adhesion assembly	ISO Inferred from Sequence Orthology more info
involved_in intracellular protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular protein transport	ISO Inferred from Sequence Orthology more info
involved_in lamellipodium organization	ISO Inferred from Sequence Orthology more info
involved_in modification of postsynaptic actin cytoskeleton	ISO Inferred from Sequence Orthology more info
involved_in modification of postsynaptic structure	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of extrinsic apoptotic signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in neuron projection morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of actin filament polymerization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of smooth muscle contraction	ISO Inferred from Sequence Orthology more info
involved_in receptor-mediated endocytosis	ISO Inferred from Sequence Orthology more info
involved_in regulation of actin filament polymerization	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of axon extension	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell projection assembly	ISO Inferred from Sequence Orthology more info
involved_in regulation of mitophagy	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within substrate-dependent cell migration, cell extension	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
colocalizes_with actin filament	IBA Inferred from Biological aspect of Ancestor more info
colocalizes_with actin filament	ISO Inferred from Sequence Orthology more info
located_in anchoring junction	IEA Inferred from Electronic Annotation more info
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	ISO Inferred from Sequence Orthology more info
located_in cell junction	ISO Inferred from Sequence Orthology more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in clathrin-coated pit	ISO Inferred from Sequence Orthology more info
is_active_in cortical actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in cortical cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
colocalizes_with focal adhesion	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
colocalizes_with growth cone	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
is_active_in lamellipodium	IBA Inferred from Biological aspect of Ancestor more info
located_in lamellipodium	IDA Inferred from Direct Assay more info	PubMed
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitotic spindle midzone	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in podosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynapse	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic actin cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in postsynaptic actin cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in ruffle	IDA Inferred from Direct Assay more info	PubMed
is_active_in site of polarized growth	IBA Inferred from Biological aspect of Ancestor more info
located_in synapse	IEA Inferred from Electronic Annotation more info
part_of voltage-gated potassium channel complex	IDA Inferred from Direct Assay more info	PubMed
part_of voltage-gated potassium channel complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: src substrate cortactin

Names: mammary tumor and squamous cell carcinoma associated (p80/85 src substrate)

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001252572.2 → NP_001239501.1 src substrate cortactin isoform 2

See identical proteins and their annotated locations for NP_001239501.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate exon in the coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1.

Source sequence(s)

AC140268

Consensus CDS

CCDS57599.1

UniProtKB/TrEMBL

Q8BNA5, Q921L6

Related

ENSMUSP00000033407.7, ENSMUST00000033407.13

Conserved Domains (3) summary

cd11959
Location:455 → 507

SH3_Cortactin; Src homology 3 domain of Cortactin

pfam02218
Location:231 → 266

HS1_rep; Repeat in HS1/Cortactin

cl05005
Location:299 → 362

TAF4; TATA Binding Protein (TBP) Associated Factor 4 (TAF4) is one of several TAFs that bind TBP and is involved in forming Transcription Factor IID (TFIID) complex
NM_001357116.2 → NP_001344045.1 src substrate cortactin isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks two alternate exons in the coding region, compared to variant 1. The resulting protein (isoform 3) is shorter when it is compared to isoform 1.

Source sequence(s)

AC140268

UniProtKB/TrEMBL

Q8BNA5

Conserved Domains (4) summary

cd11959
Location:418 → 470

SH3_Cortactin; Src homology 3 domain of Cortactin

pfam02218
Location:157 → 192

HS1_rep; Repeat in HS1/Cortactin

pfam15927
Location:285 → 326

Casc1_N; Cancer susceptibility candidate 1 N-terminus

cl00489
Location:272 → 368

60KD_IMP; 60Kd inner membrane protein
NM_007803.6 → NP_031829.2 src substrate cortactin isoform 1

See identical proteins and their annotated locations for NP_031829.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1).

Source sequence(s)

AC140268

Consensus CDS

CCDS22049.1

UniProtKB/Swiss-Prot

Q3UGC2, Q60598

UniProtKB/TrEMBL

Q8BNA5

Related

ENSMUSP00000099368.4, ENSMUST00000103079.4

Conserved Domains (3) summary

cd11959
Location:492 → 544

SH3_Cortactin; Src homology 3 domain of Cortactin

pfam02218
Location:157 → 192

HS1_rep; Repeat in HS1/Cortactin

cl05005
Location:336 → 399

TAF4; TATA Binding Protein (TBP) Associated Factor 4 (TAF4) is one of several TAFs that bind TBP and is involved in forming Transcription Factor IID (TFIID) complex