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Cpa3 carboxypeptidase A3, mast cell [ Mus musculus (house mouse) ]

Gene ID: 12873, updated on 22-Apr-2024

Summary

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Official Symbol
Cpa3provided by MGI
Official Full Name
carboxypeptidase A3, mast cellprovided by MGI
Primary source
MGI:MGI:88479
See related
Ensembl:ENSMUSG00000001865 AllianceGenome:MGI:88479
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
MC-CPA
Summary
This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases and preproprotein that is proteolytically processed to generate a mature protein product. This product is released by mast cells and may be involved in the degradation of endogenous proteins and the inactivation of venom-associated peptides. Homozygous knockout mice for this gene exhibit impaired mast cell development. [provided by RefSeq, Aug 2015]
Expression
Biased expression in limb E14.5 (RPKM 23.7), mammary gland adult (RPKM 5.5) and 4 other tissues See more
Orthologs
human all
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Genomic context

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Location:
3 A2; 3 6.25 cM
Exon count:
11
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 3 NC_000069.7 (20269780..20296373, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 3 NC_000069.6 (20215616..20242209, complement)

Chromosome 3 - NC_000069.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E1018 Neighboring gene helicase-like transcription factor Neighboring gene STARR-seq mESC enhancer starr_07108 Neighboring gene VISTA enhancer mm86 Neighboring gene glycogenin 1 Neighboring gene STARR-seq mESC enhancer starr_07112 Neighboring gene STARR-seq mESC enhancer starr_07113 Neighboring gene predicted gene, 31320 Neighboring gene microRNA 7007 Neighboring gene ribosomal protein S2 pseudogene Neighboring gene STARR-seq mESC enhancer starr_07114 Neighboring gene carboxypeptidase B1 Neighboring gene angiotensin II receptor, type 1b

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Duodenal acidification induces gastric relaxation and alters epithelial barrier function by a mast cell independent mechanism. Vanheel H, et al. Sci Rep, 2020 Oct 15. PMID 33060783, Free PMC Article
  2. Defective bone repair in mast cell-deficient Cpa3Cre/+ mice. Ramirez-GarciaLuna JL, et al. PLoS One, 2017. PMID 28350850, Free PMC Article
  3. Inhibition of inflammatory arthritis using fullerene nanomaterials. Dellinger AL, et al. PLoS One, 2015. PMID 25879437, Free PMC Article
  4. Loss of histochemical identity in mast cells lacking carboxypeptidase A. Feyerabend TB, et al. Mol Cell Biol, 2005 Jul. PMID 15988029, Free PMC Article
  5. Isolation and molecular cloning of mast cell carboxypeptidase A. A novel member of the carboxypeptidase gene family. Reynolds DS, et al. J Biol Chem, 1989 Nov 25. PMID 2584208

See all (64) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Disruption of the mast cell carboxypeptidase A3 gene does not attenuate airway inflammation and hyperresponsiveness in two mouse models of asthma.
    Title: Disruption of the mast cell carboxypeptidase A3 gene does not attenuate airway inflammation and hyperresponsiveness in two mouse models of asthma.
  2. The goal of the current study was to compare bone repair in normal wild type and Cpa3Cre/+ mice, which lack mast cells in the absence of any other hematopoietic or non- hematopoietic deficiencies. Mast cells appear in healing bone of wild type mice but not Cpa3Cre/+ mice, beginning 14 days after surgery. Re-vascularization of repair tissue and deposition of mineralized bone was delayed and dis-organised in Cpa3Cre/+ mice
    Title: Defective bone repair in mast cell-deficient Cpa3Cre/+ mice.
  3. Mast cells in experimental arthritis contained high levels of mMC-CPA mRNA, and also lacked the exopeptidase.
    Title: Experimental Arthritis Is Dependent on Mouse Mast Cell Protease-5.
  4. Data suggest that carboxypeptidase A3 (Cpa3)-Cre; myeloid cell leukemia sequence 1 (Mcl-1)(fl/fl) mice may be useful in analyzing the roles of mast cells and basophils in health and disease.
    Title: Reduced mast cell and basophil numbers and function in Cpa3-Cre; Mcl-1fl/fl mice.
  5. Mc-cpa plays a crucial role for the generation of phenotypically mature mast cells.
    Title: Loss of histochemical identity in mast cells lacking carboxypeptidase A.
  6. cathepsin E is located in mast-cell secretory granules in complex with heparin proteoglycans, and has a role in the processing of procarboxypeptidase A into active protease
    Title: A role for cathepsin E in the processing of mast-cell carboxypeptidase A.
  7. mast cell chymase and CPA have key roles in both the generation and degradation of Ang II
    Title: Cooperation between mast cell carboxypeptidase A and the chymase mouse mast cell protease 4 in the formation and degradation of angiotensin II.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables carboxypeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metallocarboxypeptidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables metallocarboxypeptidase activity ISO
Inferred from Sequence Orthology
more info
  
enables metallopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables peptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in proteolysis IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within regulation of angiotensin levels in blood IGI
Inferred from Genetic Interaction
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasmic vesicle IEA
Inferred from Electronic Annotation
more info
  
is_active_in extracellular space IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
mast cell carboxypeptidase A
NP_031779.1
XP_006535445.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_007753.2 → NP_031779.1  mast cell carboxypeptidase A preproprotein

    See identical proteins and their annotated locations for NP_031779.1

    Status: REVIEWED

    Source sequence(s)
    BY113342, BY584193, J05118
    Consensus CDS
    CCDS17262.1
    UniProtKB/Swiss-Prot
    P15089
    UniProtKB/TrEMBL
    Q542E3
    Related
    ENSMUSP00000001921.2, ENSMUST00000001921.3
    Conserved Domains (2) summary
    pfam02244
    Location:28 → 102
    Propep_M14; Carboxypeptidase activation peptide
    cl11393
    Location:114 → 413
    Peptidase_M14_like; M14 family of metallocarboxypeptidases and related proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000069.7 Reference GRCm39 C57BL/6J

    Range
    20269780..20296373 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006535382.4 → XP_006535445.1  mast cell carboxypeptidase A isoform X1

    Conserved Domains (2) summary
    pfam02244
    Location:32 → 103
    Propep_M14; Carboxypeptidase activation peptide
    cl11393
    Location:114 → 330
    Peptidase_M14_like; M14 family of metallocarboxypeptidases and related proteins
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P15089.1 GenPept UniProtKB/Swiss-Prot:P15089

Gene LinkOut

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