Chrm3 cholinergic receptor, muscarinic 3, cardiac [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Chrm3provided by MGI
Official Full Name: cholinergic receptor, muscarinic 3, cardiacprovided by MGI
Primary source: MGI:MGI:88398
See related: Ensembl:ENSMUSG00000046159 AllianceGenome:MGI:88398
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: M3; M3R; Chrm-3
Summary: Predicted to enable several functions, including G protein-coupled acetylcholine receptor binding activity; G protein-coupled amine receptor activity; and acetylcholine binding activity. Acts upstream of or within several processes, including cholinergic synaptic transmission; positive regulation of smooth muscle contraction; and saliva secretion. Predicted to be located in asymmetric synapse; axon terminus; and basal plasma membrane. Predicted to be active in dendrite and synapse. Predicted to be integral component of plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Used to study megacystis-microcolon-intestinal hypoperistalsis syndrome. Human ortholog(s) of this gene implicated in bladder disease; chronic obstructive pulmonary disease; prune belly syndrome; and pulmonary fibrosis. Orthologous to human CHRM3 (cholinergic receptor muscarinic 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in cortex adult (RPKM 8.4), bladder adult (RPKM 7.2) and 17 other tissues See more
Orthologs: human all
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Genomic context

Location:: 13 A1; 13 3.72 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	13	NC_000079.7 (9925522..10411131, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	13	NC_000079.6 (9875486..10361062, complement)

Chromosome 13 - NC_000079.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The M3 Muscarinic Acetylcholine Receptor Promotes Epidermal Differentiation.
Title: The M3 Muscarinic Acetylcholine Receptor Promotes Epidermal Differentiation.
Pancreas-specific CHRM3 activation causes pancreatitis in mice.
Title: Pancreas-specific CHRM3 activation causes pancreatitis in mice.
Muscarinic receptor M3 contributes to intestinal stem cell maintenance via EphB/ephrin-B signaling.
Title: Muscarinic receptor M3 contributes to intestinal stem cell maintenance via EphB/ephrin-B signaling.
Inhibition of CHRM3 Alleviates Necrosis Via the MAPK-p38/miR-31-5p/RIP3 Axis in L-Arginine-Induced Severe Acute Pancreatitis.
Title: Inhibition of CHRM3 Alleviates Necrosis Via the MAPK-p38/miR-31-5p/RIP3 Axis in L-Arginine-Induced Severe Acute Pancreatitis.
Functions of Muscarinic Receptor Subtypes in Gastrointestinal Smooth Muscle: A Review of Studies with Receptor-Knockout Mice.
Title: Functions of Muscarinic Receptor Subtypes in Gastrointestinal Smooth Muscle: A Review of Studies with Receptor-Knockout Mice.
Aged Mice Devoid of the M3 Muscarinic Acetylcholine Receptor Develop Mild Dry Eye Disease.
Title: Aged Mice Devoid of the M(3) Muscarinic Acetylcholine Receptor Develop Mild Dry Eye Disease.
Deletion of muscarinic acetylcholine receptor 3 in microglia impacts brain ischemic injury.
Title: Deletion of muscarinic acetylcholine receptor 3 in microglia impacts brain ischemic injury.
A Chrm3 missense T1667C mutation in the last intracellular domain functionally correlated with a rise in BP increased the M3R signalling and resensitization, and adrenal epinephrogenesis. Gene targeting that abolished the M3R function without affecting any of noncoding Chrm3 variants caused a decrease in BP, indicating that the M3R-mediated signalling promotes hypertension.
Title: Functional Dosage of Muscarinic Cholinergic Receptor 3 Signalling, Not the Gene Dose, Determines Its Hypertension Pathogenesis.
Chrm1 and Chrm3 double knockout chronically diminishes REM sleep to an almost undetectable level. These results suggest that muscarinic acetylcholine receptors, Chrm1 and Chrm3, are essential for REM sleep.
Title: Muscarinic Acetylcholine Receptors Chrm1 and Chrm3 Are Essential for REM Sleep.
Genetic M3R ablation in mouse cells results in improved remyelination and is mediated by acceleration of oligodendrocyte commitment from oligodendrocyte progenitor cells.
Title: Muscarinic Receptor M(3)R Signaling Prevents Efficient Remyelination by Human and Mouse Oligodendrocyte Progenitor Cells.

Submit: New GeneRIF Correction See all GeneRIFs (81)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (5) 1 citation
Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D13Mit172 (e-PCR), detects polymorphism
- UniSTS:126988

03.MMHAP32FLA3.seq (e-PCR)
- UniSTS:160270

D13Mit215 (e-PCR), detects polymorphism
- UniSTS:127031

Chrm3 (e-PCR), detects polymorphism
- UniSTS:226181

44.MMHAP91FLG5.seq (e-PCR)
- UniSTS:124556

D7S2508 (e-PCR), detects polymorphism
- UniSTS:75161

MHAa7c12.seq (e-PCR)
- UniSTS:125342

Chrm3 (e-PCR), detects polymorphism
- UniSTS:506797

fb54b04.x1 (e-PCR)
- UniSTS:190695

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables G protein-coupled acetylcholine receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables G protein-coupled acetylcholine receptor activity	IDA Inferred from Direct Assay more info	PubMed
enables G protein-coupled acetylcholine receptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables G protein-coupled acetylcholine receptor activity	ISO Inferred from Sequence Orthology more info
enables G protein-coupled acetylcholine receptor binding	ISO Inferred from Sequence Orthology more info
enables G protein-coupled receptor activity	IEA Inferred from Electronic Annotation more info
enables G protein-coupled serotonin receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables acetylcholine binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables quaternary ammonium group binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in G protein-coupled acetylcholine receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger	IBA Inferred from Biological aspect of Ancestor more info
involved_in acetylcholine receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within calcium-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in chemical synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in ion channel modulating, G protein-coupled receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in ligand-gated ion channel signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of heart rate by acetylcholine	ISO Inferred from Sequence Orthology more info
involved_in phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of insulin secretion	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of insulin secretion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of smooth muscle contraction	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of vascular associated smooth muscle contraction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of vasoconstriction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of monoatomic ion transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
involved_in regulation of smooth muscle contraction	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to acetylcholine	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within saliva secretion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of smooth muscle contraction	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within smooth muscle contraction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within synaptic transmission, cholinergic	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in asymmetric synapse	ISO Inferred from Sequence Orthology more info
located_in axon terminus	ISO Inferred from Sequence Orthology more info
located_in basal plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in dendrite	IBA Inferred from Biological aspect of Ancestor more info
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	TAS Traceable Author Statement more info
is_active_in postsynaptic density membrane	ISO Inferred from Sequence Orthology more info
located_in postsynaptic membrane	IEA Inferred from Electronic Annotation more info
is_active_in presynaptic membrane	ISO Inferred from Sequence Orthology more info
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: muscarinic acetylcholine receptor M3

Names: AChR M3; mm3 mAChR; muscarinic acetylcholine receptor 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001428369.1 → NP_001415298.1 muscarinic acetylcholine receptor M3

Status: VALIDATED

Source sequence(s)

AC122288, AC124429, AC146596, AC158115

UniProtKB/Swiss-Prot

Q64055, Q9ERZ3

UniProtKB/TrEMBL

Q542R4
NM_001428370.1 → NP_001415299.1 muscarinic acetylcholine receptor M3

Status: VALIDATED

Source sequence(s)

AC122288, AC124429, AC140445, AC146596, AC158115

UniProtKB/Swiss-Prot

Q64055, Q9ERZ3

UniProtKB/TrEMBL

Q542R4
NM_033269.5 → NP_150372.1 muscarinic acetylcholine receptor M3

See identical proteins and their annotated locations for NP_150372.1

Status: VALIDATED

Source sequence(s)

AC122288, AC124429, AC146596, AC158115

Consensus CDS

CCDS26238.1

UniProtKB/Swiss-Prot

Q64055, Q9ERZ3

UniProtKB/TrEMBL

Q542R4

Related

ENSMUSP00000140131.2, ENSMUST00000187510.7

Conserved Domains (3) summary

cd15299
Location:65 → 269

7tmA_mAChR_M3; muscarinic acetylcholine receptor subtype M3, member of the class A family of seven-transmembrane G protein-coupled receptors

cd14964
Location:486 → 511

7tm_GPCRs; TM helix 6 [structural motif]

cl28897
Location:483 → 563

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000079.7 Reference GRCm39 C57BL/6J

Range

9925522..10411131 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006516468.5 → XP_006516531.1 muscarinic acetylcholine receptor M3 isoform X1

Conserved Domains (3) summary

cd15299
Location:65 → 269

7tmA_mAChR_M3; muscarinic acetylcholine receptor subtype M3, member of the class A family of seven-transmembrane G protein-coupled receptors

cd14964
Location:486 → 511

7tm_GPCRs; TM helix 6 [structural motif]

cl28897
Location:483 → 563

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily