Chek1 checkpoint kinase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Chek1provided by MGI
Official Full Name: checkpoint kinase 1provided by MGI
Primary source: MGI:MGI:1202065
See related: Ensembl:ENSMUSG00000032113 AllianceGenome:MGI:1202065
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Chk1; rad27
Summary: Enables histone kinase activity (H3-T11 specific). Involved in epigenetic maintenance of chromatin in transcription-competent conformation; regulation of histone H3-K9 acetylation; and regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage. Acts upstream of or within several processes, including DNA damage checkpoint signaling; G2/M transition of mitotic cell cycle; and inner cell mass cell proliferation. Located in chromatin; nucleus; and replication fork. Is expressed in several structures, including adrenal medulla; early conceptus; gonad; gut; and trunk mesenchyme derived from neural crest. Human ortholog(s) of this gene implicated in pancreatic cancer. Orthologous to human CHEK1 (checkpoint kinase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in CNS E11.5 (RPKM 8.0), liver E14 (RPKM 5.6) and 9 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 A4; 9 20.67 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (36619935..36637897, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (36708482..36726658, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

SIRT3-dependent mitochondrial redox homeostasis mitigates CHK1 inhibition combined with gemcitabine treatment induced cardiotoxicity in hiPSC-CMs and mice.
Title: SIRT3-dependent mitochondrial redox homeostasis mitigates CHK1 inhibition combined with gemcitabine treatment induced cardiotoxicity in hiPSC-CMs and mice.
Inhibition of RRM2 radiosensitizes glioblastoma and uncovers synthetic lethality in combination with targeting CHK1.
Title: Inhibition of RRM2 radiosensitizes glioblastoma and uncovers synthetic lethality in combination with targeting CHK1.
Knockdown of Chk1 inhibits proliferation and promotes apoptosis in mouse granulosa cells and its regulation mechanism by miR-15a and miR-16.
Title: Knockdown of Chk1 inhibits proliferation and promotes apoptosis in mouse granulosa cells and its regulation mechanism by miR-15a and miR-16.
Chk1 Inhibition Ameliorates Alzheimer's Disease Pathogenesis and Cognitive Dysfunction Through CIP2A/PP2A Signaling.
Title: Chk1 Inhibition Ameliorates Alzheimer's Disease Pathogenesis and Cognitive Dysfunction Through CIP2A/PP2A Signaling.
ChK1 activation induces reactive astrogliosis through CIP2A/PP2A/STAT3 pathway in Alzheimer's disease.
Title: ChK1 activation induces reactive astrogliosis through CIP2A/PP2A/STAT3 pathway in Alzheimer's disease.
PSPC1 regulates CHK1 phosphorylation through phase separation and participates in mouse oocyte maturation.
Title: PSPC1 regulates CHK1 phosphorylation through phase separation and participates in mouse oocyte maturation.
The Atr-Chek1 pathway inhibits axon regeneration in response to Piezo-dependent mechanosensation.
Title: The Atr-Chek1 pathway inhibits axon regeneration in response to Piezo-dependent mechanosensation.
Small molecule inhibitors and a kinase-dead expressing mouse model demonstrate that the kinase activity of Chk1 is essential for mouse embryos and cancer cells.
Title: Small molecule inhibitors and a kinase-dead expressing mouse model demonstrate that the kinase activity of Chk1 is essential for mouse embryos and cancer cells.
Phosphoproteomic Analysis of Neonatal Regenerative Myocardium Revealed Important Roles of Checkpoint Kinase 1 via Activating Mammalian Target of Rapamycin C1/Ribosomal Protein S6 Kinase b-1 Pathway.
Title: Phosphoproteomic Analysis of Neonatal Regenerative Myocardium Revealed Important Roles of Checkpoint Kinase 1 via Activating Mammalian Target of Rapamycin C1/Ribosomal Protein S6 Kinase b-1 Pathway.
CHK1 monitors spindle assembly checkpoint and DNA damage repair during the first cleavage of mouse early embryos.
Title: CHK1 monitors spindle assembly checkpoint and DNA damage repair during the first cleavage of mouse early embryos.

Submit: New GeneRIF Correction See all GeneRIFs (63)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (7) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Chek1 (e-PCR), detects polymorphism
- UniSTS:532481

RH127224 (e-PCR)
- UniSTS:209799

PMC316686P1 (e-PCR)
- UniSTS:273037

PMC316691P1 (e-PCR)
- UniSTS:273038

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables histone H3T11 kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables histone H3T11 kinase activity	ISO Inferred from Sequence Orthology more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage checkpoint signaling	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA damage checkpoint signaling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within DNA damage response	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA damage response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within DNA repair	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA repair	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within G2/M transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within apoptotic process involved in development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within inner cell mass cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic G2 DNA damage checkpoint signaling	ISO Inferred from Sequence Orthology more info
involved_in mitotic G2/M transition checkpoint	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within mitotic cell cycle checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of G2/M transition of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of gene expression, epigenetic	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of mitotic nuclear division	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within nucleus organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidyl-threonine phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cell cycle	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of double-strand break repair via homologous recombination	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of gene expression	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of mitotic centrosome separation	ISO Inferred from Sequence Orthology more info
involved_in signal transduction in response to DNA damage	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in centrosome	ISO Inferred from Sequence Orthology more info
part_of chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
colocalizes_with chromosome, telomeric region	ISO Inferred from Sequence Orthology more info
located_in condensed nuclear chromosome	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
located_in replication fork	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: serine/threonine-protein kinase Chk1

Names: CHK1 checkpoint homolog; checkpoint kinase 1 homolog; rad27 homolog

NP_001397134.1

EC 2.7.11.1

NP_001397135.1

EC 2.7.11.1

NP_001397136.1

EC 2.7.11.1

NP_031717.2

EC 2.7.11.1

XP_017168606.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001410205.1 → NP_001397134.1 serine/threonine-protein kinase Chk1 isoform 2

Status: VALIDATED

Source sequence(s)

AC155921
NM_001410206.1 → NP_001397135.1 serine/threonine-protein kinase Chk1 isoform 3

Status: VALIDATED

Source sequence(s)

AC155921
NM_001410207.1 → NP_001397136.1 serine/threonine-protein kinase Chk1 isoform 1

Status: VALIDATED

Source sequence(s)

AC155921

UniProtKB/Swiss-Prot

O35280, O54925, Q8CI40, Q9D0N2
NM_007691.6 → NP_031717.2 serine/threonine-protein kinase Chk1 isoform 1

See identical proteins and their annotated locations for NP_031717.2

Status: VALIDATED

Source sequence(s)

AC155921

Consensus CDS

CCDS22972.1

UniProtKB/Swiss-Prot

O35280, O54925, Q8CI40, Q9D0N2

UniProtKB/TrEMBL

Q497T8

Related

ENSMUSP00000034625.6, ENSMUST00000034625.12

Conserved Domains (1) summary

cd14069
Location:7 → 265

STKc_Chk1; Catalytic domain of the Serine/Threonine kinase, Checkpoint kinase 1

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

36619935..36637897 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017313117.3 → XP_017168606.1 serine/threonine-protein kinase Chk1 isoform X1

Conserved Domains (1) summary

cl21453
Location:1 → 164

PKc_like; Protein Kinases, catalytic domain