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Aup1 ancient ubiquitous protein 1 [ Mus musculus (house mouse) ]

Gene ID: 11993, updated on 11-Apr-2024

Summary

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Official Symbol
Aup1provided by MGI
Official Full Name
ancient ubiquitous protein 1provided by MGI
Primary source
MGI:MGI:107789
See related
Ensembl:ENSMUSG00000068328 AllianceGenome:MGI:107789
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary
The protein encoded by this gene contains several conserved domains including a hydrophobic domain, an acetyltransferase domain, a ubiquitin binding domain, and a domain required for recruitment of ubiquitin-conjugating enzyme E2 G2 (Ube2g2). In humans, this protein localizes to the endoplasmic reticulum and to lipid droplets. This protein is thought to be involved both in the degradation of misfolded proteins from the endoplasmic reticulum and in the storage of neutral lipids. Reduced expression of the human ortholog of this gene strongly reduces lipid droplet clustering in the cell, and causes stabilization of misfolded proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]
Expression
Ubiquitous expression in adrenal adult (RPKM 174.0), duodenum adult (RPKM 139.9) and 28 other tissues See more
Orthologs
human all
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Genomic context

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Location:
6 C3; 6 35.94 cM
Exon count:
12
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (83031483..83034663)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (83054495..83057682)

Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_16455 Neighboring gene docking protein 1 Neighboring gene lysyl oxidase-like 3 Neighboring gene STARR-positive B cell enhancer ABC_E2791 Neighboring gene microRNA 7040 Neighboring gene STARR-positive B cell enhancer ABC_E9275 Neighboring gene HtrA serine peptidase 2 Neighboring gene DEAQ RNA-dependent ATPase Neighboring gene T cell leukemia, homeobox 2

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Monoubiquitination of ancient ubiquitous protein 1 promotes lipid droplet clustering. Lohmann D, et al. PLoS One, 2013. PMID 24039768, Free PMC Article
  2. Aup1, a novel gene on mouse chromosome 6 and human chromosome 2p13. Jang W, et al. Genomics, 1996 Sep 1. PMID 8812468
  3. DQX1, an RNA-dependent ATPase homolog with a novel DEAQ box: expression pattern and genomic sequence comparison of the human and mouse genes. Ji W, et al. Mamm Genome, 2001 Jun. PMID 11353393
  4. Ancient ubiquitous protein-1 mediates sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl CoA reductase in lipid droplet-associated endoplasmic reticulum membranes. Jo Y, et al. Mol Biol Cell, 2013 Feb. PMID 23223569, Free PMC Article
  5. High-resolution genetic, physical, and transcript map of the mnd2 region of mouse chromosome 6. Weber JS, et al. Genomics, 1998 Nov 15. PMID 9806835

See all (27) citations in PubMed

GeneRIFs: Gene References Into Functions

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ubiquitin binding IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin conjugating enzyme binding ISO
Inferred from Sequence Orthology
more info
  
enables ubiquitin protein ligase binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in ERAD pathway IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in ERAD pathway ISO
Inferred from Sequence Orthology
more info
  
involved_in lipid droplet formation ISO
Inferred from Sequence Orthology
more info
  
involved_in lipid droplet organization ISO
Inferred from Sequence Orthology
more info
  
involved_in lipophagy ISO
Inferred from Sequence Orthology
more info
  
involved_in protein localization to lipid droplet ISO
Inferred from Sequence Orthology
more info
  
involved_in response to virus ISO
Inferred from Sequence Orthology
more info
  
involved_in retrograde protein transport, ER to cytosol ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
located_in autophagosome ISO
Inferred from Sequence Orthology
more info
  
located_in endoplasmic reticulum ISO
Inferred from Sequence Orthology
more info
  
is_active_in endoplasmic reticulum membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum membrane ISO
Inferred from Sequence Orthology
more info
  
located_in lipid droplet ISO
Inferred from Sequence Orthology
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
lipid droplet-regulating VLDL assembly factor AUP1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001301649.1 → NP_001288578.1  lipid droplet-regulating VLDL assembly factor AUP1 isoform 2

    See identical proteins and their annotated locations for NP_001288578.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an alternate exon in the coding region and uses a downstream start codon compared to variant 1. It encodes isoform 2 which has a shorter N-terminus compared to isoform 1.
    Source sequence(s)
    AK158035, BM900175, BY234324
    UniProtKB/TrEMBL
    I6L970
    Conserved Domains (2) summary
    cd14420
    Location:167 → 211
    CUE_AUP1; CUE domain found in ancient ubiquitous protein 1 (AUP1) and similar proteins
    cl17185
    Location:33 → 135
    LPLAT; Lysophospholipid acyltransferases (LPLATs) of glycerophospholipid biosynthesis

  2. NM_007517.5 → NP_031543.3  lipid droplet-regulating VLDL assembly factor AUP1 isoform 1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longer isoform (1).
    Source sequence(s)
    AC104324
    Consensus CDS
    CCDS39526.1
    UniProtKB/Swiss-Prot
    P70295
    UniProtKB/TrEMBL
    Q3TAZ9, Q3TBX5
    Related
    ENSMUSP00000090281.8, ENSMUST00000092618.10
    Conserved Domains (2) summary
    cd14420
    Location:296 → 340
    CUE_AUP1; CUE domain found in ancient ubiquitous protein 1 (AUP1) and similar proteins
    cl17185
    Location:66 → 264
    LPLAT; Lysophospholipid acyltransferases (LPLATs) of glycerophospholipid biosynthesis

RNA

  1. NR_125891.1 RNA Sequence

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) uses an alternate splice site at an internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    AK158035, AK167335, BM900175, BY234324
    Related
    ENSMUST00000203915.4

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000072.7 Reference GRCm39 C57BL/6J

    Range
    83031483..83034663
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

RNA

  1. XR_377416.4 RNA Sequence

  2. XR_377415.4 RNA Sequence

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_001025446.1: Suppressed sequence

    Description
    NM_001025446.1: This RefSeq was permanently suppressed because it is a nonsense-mediated mRNA decay (NMD) candidate.
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P70295.1 GenPept UniProtKB/Swiss-Prot:P70295

Gene LinkOut

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